The principle of SDS PAGE-a full and clear explanation of the technique and how does it work

This video is to understand everything about SDS-PAGE, its principle, the technique, the discontinuous gel system, and more.
2D gel Electrophoresis video:
• The principle of 2D Ge...
Western Blotting video:
• Blotting Techniques/ T...

[@Kevinsungeun]

Why can't my professor just memorise this video and rap it during the lab. Very useful!

[@biomedicalandbiologicalsci4989]

Haha thank you ... maybe you can recommend it to your colleagues ;)

[@MrPridizzle]

throw that ish on Rap Chat yo, that would be FIRE!

[@julietten5614]

your professor probs done thousands of 'gels' while doing his first research work. I m assuming he's just not interested any more:-))

[@tartanhandbag]

this is why professors should do research and teachers should teach. not always the case; some professors are excellent, lively, dynamic and patient teachers also ...but most just want to get back to overseeing their projects, publishing papers and finishing grant applications before the dreaded deadlines.

[@jimmyolum4344]

Hehe

[@gabrieldynkin5078]

I just have to say it was the best explantion i've ever heard about anything! Thank you, it answered all of my questions....:)

[@4MoonGlade4]

Hello!
This video really saved me!
Detailed and exhaustive.
I was studying this technique for an exam and didn't understand it really...now it's all clear.
Thanks! 😊

[@brindabandas3341]

This is the first technique video which I have completely understood without any practical experience. Thank you so much for making such a great video.

[@ismailgbadamosi8321]

This is the best video I have seen on the working principle of SDS PAGE. Thank you

[@saniyapamnani8494]

This is the best video I found on the internet about SDS PAGE. Thank you so much for all the details.

[@medinlab141]

Thank you so much for the explanation, especially for the stacking gel's part! I found it very clear.

[@justcat6042]

This is the most helpful video i have ever watched about sds page. Now i understand much more about this! Thank you so much

[@ranahaddad5446]

I had watched many videos to understand this topic, but this video is definitely the best! Thank you so much!

[@murielmirus5641]

thank you so much for the hard work you put into this video! it is very much appreciated :) your video has helped me a lot in the lab today!

[@RevesAbsurdes1]

Perfect ! I spend time looking for those informtion on internet and you give them (and a lot more) very clearly, good job !

[@biomedicalandbiologicalsci4989]

Hello, thank you for your comment ... happy you got what you need from the video :)

[@RevesAbsurdes1]

Biomedical and Biological Sciences yes i actually have an exam about all those technics soon and you saved my life haha !

[@biomedicalandbiologicalsci4989]

Wow, this is good ... I have a playlist on my channel containing videos about all the electrophoresis techniques, have a look at it, it will help you for your exam:
ruclips.net/video/On_ZotdZexI/видео.html

[@UQMD]

Thanks for the fantastic video! We just watched your video in class and it definitely helped us understand electrophoresis. I especially loved the pace of your video :)

[@tedwills-topic9524]

This helped me a lot before even doing my practicals. Thank you

[@maharmibhattacharya4327]

thank you so much, the explanation was very descriptive and reasonably good.

[@daniellegoodwin3517]

Thanks so much for a very clear explanation! I tried to understand the technique by reading a description online but it was so confusing, thanks for clearing it up for me!

[@harveenkaur1713]

The most helpful video ever! Thank you so much!

[@KJKP]

More detailed than other videos on SDS-PAGE.

[@biomedicalandbiologicalsci4989]

yup, stay tuned for more videos :)

[@julymgreenday1993]

I finally undertood Electrophoresis!!!! THANK YOU! Greetings from Argentina ♥

[@biomedicalandbiologicalsci4989]

Hei, Glade you liked the video ... stay tuned :)

[@rogsang9764]

thanks for the explanation, the best thing is that you dedicate time to explain the principle of the gel, its the best explanation i´ve seen so far :D

[@ZAIUAMED]

Very helpful keep it up.

[@sumantahalder1027]

I am too pleased to watch your videos, these are very clear concept growing video. Thank you very much keep it up.

[@biomedicalandbiologicalsci4989]

Thank you for your comment ... I will ... Stay tuned :)

[@mariakln9654]

Congrats! Very helpful video, I understood everything I needed for my lab. Thank you! (the explanation is very good and well constructed)

[@swagpunjabiada6521]

Mam please keep uploading such videos so that we can have clear concept about each technique ur way of teaching is great😄

[@shreyasen5614]

Love the video! Very informative. Thank you so much

[@giorgiasartori6334]

you are so amazing, keep going like this!! this is THE ONLY VIDEO i found explained well

[@francestorreysirdevan4774]

Superb video!! Insanely helpful! Thank you so much!!

[@richardjoy8522]

Really really really good explanation...I was searching for this type of detailed explanation in whole of RUclips.....

[@MuhammadRizwan-dt5us]

i find it very useful thankyou so much for make that kind of videos

[@roulala8993]

Thank you very much for the video. You're a good teacher. 😊

[@joannchu14]

Beautiful explanation! Love this ❤

[@punithagr3339]

Clear and to the point. Thank you

[@avishekdas180]

Nicely done yet again.

[@ashiqahamed7740]

Very well put together. loved this video Thank you :)

[@allaboutfootball5729]

Thank u very much u r wonderful keep making these videos I learnt a lot and many things become clear

[@bobmvideos]

OMG thanks, my wife has been doing these for like 25 years, now I know what the hell she has been talking about. Now to watch your video on westerns...

[@claudiaaurie8161]

very good explanation. Thank u!

[@Laladear-sz4hz]

thanku for clearing my doubts about this techniques

[@simiralucialamberty5971]

Your videos are excellent!

[@sundasshareef8691]

Perfect .👍 simply wht i was actually searching for🙌

[@orfeasorfeas9346]

I finally understood it! Thanks!

[@soniyahalan9051]

Thank you so much video is very helpful to understand better SDS page

[@allysadati9710]

True

[@dancingbiologist2688]

This was very helpful! Thank you!

[@alfankoshable1]

thank you very informative ! keep going from Saudi arabia Salam

[@abidakhadim1935]

Thank you mam .for giving a good visual memory of SDS.page .

[@preciousndzimandze4348]

So helpful, thanks so much!

[@priyadharshini-jx6jz]

So clear ,good job 👍

[@physicstutor9127]

Very helpful. Thank you!

[@TheQaseeh]

thank you!! clear and detail explanation....

[@colin-kun3611]

Solid video! Thanks a million times!
Subbed!

[@elmaspower2356]

Awesomely explained.. Thanks very much mam. Lots of love and respect from Bangladesh

[@danielhernandezbanuelos5581]

thank you, very well explained.

[@pavanhebbar5843]

For SDS, I just watch this... Thank you for this indepth information mam🙏🙏

[@bharathreddy9337]

I wish I will have a teacher like you in the future. terrific and detailed explanation

[@adityasinha1166]

Thankyou so much for the explanation. I loved it 😊😊. Good work.

[@aydamohamed7983]

Thnx sooooo much.
Very clear and important

[@Ash-be1nv]

extremely helpful video for beginners

[@geralthasan]

you have my thanks madam for saving me from mid exam disaster. Thank you so much

[@genomics3754]

thank you mam....for such a well explained lecture.

[@kaushikilohiya2137]

This is a really great explanation of d topic

[@emilievu4846]

Very well explained! Do you have any sources for this? I would like to refer to it in my masters.

[@trish88881]

This is so useful. Life Saver!

[@muhammadashraf-iy1gd]

Outstanding explanation ❤

[@shdrone94]

very good explained !

[@StalwartsCo]

indeed best video for beginners.

[@darkgamingyt1659]

Superb explaination

[@uzoechisamuel]

And somebody has been messing with my brain all this while. Anytime I see you in person, I will give you a bottle of win.

[@ruchinambiar8005]

This video was very useful. Thank you ma'am

[@junghyunlee1714]

Umm... I have one question. At the stacking gel, speed of the ions is Cl(-)>protein>glycine(n/-). In my opinion, Cl is faster than glycine these distance gonna be far each other. I wanna know details what is happened in stacking gel and these speed.

[@sylaslau302]

Thank you!! It's comprehensive and concise at the same time. I wonder what would be the technical difference (composition and steps) between this and Urea-PAGE for ssDNA or RNA.

[@yeny7194]

Thank you this helps a lot! the article i'm reading talks about 3 different gels running gel, gradient gel and stacking gel, In this video we are shown separating gel, which one of the two (running or gradient gels) would the separating gel be equivalent to?

[@vavakhannan3335]

Very well explained........thank you so much mam

[@deegallarupananda6927]

Thank you very much!

[@krishnanandh7999]

Very informative. Thank you😍

[@biotechnologymahasarakhamu4757]

Awesome teaching. Thank you so much.

[@biomedicalandbiologicalsci4989]

welcome ... Thank you for the comment :)

[@realmetv2970]

Such a perfect video

[@pannalalroat5394]

Really such a amazing explanation 😘🤩

[@poetrylover5561]

Very nice explanation of a difficult concept of SDS PAGE 👍👍👍👍

[@ruhiyyihdyrdahl-young3846]

Thanks what a great explanation!

[@biomedicalandbiologicalsci4989]

You are welcome :D

[@ikz8439]

you're helpful thanks!

[@leticiaalvescavalcante9593]

OH MY GOD!!!! Thank you SO much!!! I'm a student from Brazil and I was looking for something that would make me understand the electrophoresis tecnique and I was not findind anything so simple and easy like this video! I'm so happy right now... I was almost giving up haha I was reading, and reading, and reading and not understanding at all, but now is everything so clear in my mind because of you...Omg, THANK YOUUUUU!!!!!!

[@biomedicalandbiologicalsci4989]

WOOW .. thank you for the nice comment ... stay tuned, you might find everything you are searching for in this channel :)

[@elenatantardini4932]

Best WB video ever!

[@M7MD99.]

كثر الله خيرك

[@MayankSingh-uj6hi]

Very good explanation. Thank u sooo much mam

[@sadeghsadeghzadeh8170]

It was very useful. Thanks alot

[@firasbn1582]

Thank you for the Video

[@shivaprasad5446]

well explained, thanks for the video :)

[@yourdoctorpharma]

Well explained!

[@debjitdey216]

Finally I understood 🤗🤗🤗 thank uuuuuuu

[@batuhantekmen6607]

Why and how proteins have been aligned in the stacking gel? Thank you.

[@kashmirnature3134]

Amazing' ❤️❤️
Thanks ma'am❤️

[@pkjaihind3472]

Excellent Explanation 👍👏👏👏
Keep it up 👍

[@user-bc1hv2cc9p]

thank you so much it's very helpful

[@konidaladevasri4405]

Ver well explained!!

[@maharshi3180]

*THANK YOU SO SO MUCH*

[@ArielStar]

Amazingly explained, thank you!!

[@user-eq8mc2hi3y]

Oh my god so useful video... you save my grade

[@amoon4036]

very good thank you.

[@swetaxavier6290]

ThNk you for this video

[@benharratsara719]

Hi, I want to stady the expression of a big protein wich mesure 358kda using western blot. How can I prepare a separating gel with a small gradient to visualize this big protein?

[@a_m1350]

How can we distinguish the different proteins and their polymorphisms from each other after the separation.
If, say our sample is cattle milk and we are interested in casein and all it's various polymorphism alpha, beta, kappa etc. Are we going to use the same or different markers to identify the required proteins.