for the past 4 years, your channel have helped me through my hard times until i finally got my degree. Now look, this is my first time working and i'm still here. i still need you. THANK YOU MISTER!!! i hope you have a happy life! now and always!
This was a topic that could have been confusing and hard to understand but you managed to explain it clearly and it was easy to follow your explanation! Great Job!
I probably never get the chance to tell you in person that YOUR lectures are the main reason of my GPA =8 in my mater degree...YOU are just awesome man......
about F=Fv：Because friction is more complicated, it is usually some empirical formula. In the past, the friction force you were in contact with usually referred to dry friction between solids. If you consider that the object is moving in air or liquid, the measurement will find that the friction force is related to the speed. It is generally considered that at high speed, the friction force is proportional to the square of the speed, and when moving slowly, the friction force is approximately linear with the speed.
Not be a nerd, but SDS is missing one more carbon in the video (Dodecyl = 12). I only see 11 carbons in the drawing. I appreciate your work, and needed this very much.
He is right about the electric field being constant, BUT! Actually the voltage is not constant! In order to maintain a constant electric current, which generates ''almost'' constant velocity, the gel-electrophoresis machine increases the voltage during the process in order to resist the ever increasing impedance values inside the medium (anyone doing gel-electrophoresis can see that). Actually during the migration the voltage more than doubles! Does not matter in the end... Excellent video anyway!
Thanks for a great video. I have one question. How is the force of friction = f*velocity? Isn't frictional coefficient dimensionless so the unit does not come out to be Newtons?
It occurs to me that the structure formula of SDS written on the white board is incorrect (one "CH2" is missing in the dodecyl fatty acid)... But otherwise: great explaination!
I think there is a problem, the coefficient of friction must be without dimension, while here you when you multiply f with velocity it does not make sense, would please clarify it???!!!!!
Can anyone please explain Why frictional force is => frictional constant(f) X velocity (v)? Far as i know Frictional force is equal to frictional constant(f) X Normal Force (N)
amazinnnnng. can i ask a tow question: 1. whats the deference between 6x and 2x loading dye and which i should use ? 2. which voltage is optimal to run one caste of gel, i usually use 65 v. ?
the concentrations of chemicals in the 6x loading buffer is three times as high compared tot the 2x loading buffer. This is the only difference. You should dilute your protein sample with the loading buffer in such a way that you always end up with 1x loading buffer. the 2x loading buffer should be diluted 1:1 with your sample. the 6x should be diluted 1:5.
the concentrations of chemicals in the 6x loading buffer is three times as high compared tot the 2x loading buffer. This is the only difference. You should dilute your protein sample with the loading buffer in such a way that you always end up with 1x loading buffer. the 2x loading buffer should be diluted 1:1 with your sample. the 6x should be diluted 1:5.
I always thought the reason ads page was due to size not charge was because the charge to mass ratio for all proteins is the same with SDS. 10 amino acids = every other amino acid gets sds/ = arbitrary net charge of 5. Divide that by 10 = 1/2. 30 amino acid... 15 charge.. 15/30 is 1/2 .Thus all proteins have the same exact charge/mass ratio.
Farida Chy the presence of mercaptoethanol disrupts the disulfide bridge bond found in the protein when mercaptoethanol is not present the disulfide bond in protiens stay intact hope that was helpful
Mercaptoethanol is a reducing agent that cuts disulfide bonds within proteins in order to disrupt the 3-dimensional shape of proteins, a process that is called "denaturation".
I find your explantation of the net force on the proteins in the gel to be problematic. Could you direct me to the source of your explanation so that I can review it?
this guy needs to be cloned and teach every single BIO course.
doont need to clone when we can have youtube. Every single Bio course can be taken here :)
Felipee Rincon I concur 😊
🤣 "should be cloned"🤣
for the past 4 years, your channel have helped me through my hard times until i finally got my degree. Now look, this is my first time working and i'm still here. i still need you. THANK YOU MISTER!!! i hope you have a happy life! now and always!
You explained this better than both my professor and textbook. I look forward to watching your other videos!
I love how in depth you you go but are still easy to follow. Thank you!
exactly, most khan academy or other resources give you a very shallow info and you get more and more confuse, but he breaks down everything
Incredible! This guy is always giving the most helpful, concise information out there. Never a syllable wasted
Becoming a fan of yours day by day. Not everyone has this capability of making people understand complicated topics. Thank you so much
This was a topic that could have been confusing and hard to understand but you managed to explain it clearly and it was easy to follow your explanation! Great Job!
Very useful! You explain another points of view from this topic that I don't really usually found in textbook or in our lectures. Thanks a lot!
The only way Im getting through BioChem right now are these videos. Thank you so much!
love the clear diagrams and explanation, thanks!
needfire you're welcome!
The greatest teacher on RUclips! Thank you so much !!
I probably never get the chance to tell you in person that YOUR lectures are the main reason of my GPA =8 in my mater degree...YOU are just awesome man......
You rock..very simplified and clear explanation. Thank you for your efforts
definitely the best videos on youtube!! Better than Khan academy!
Literally you're the best ...your way of explaining is making the concept crystal clear... thank you AK lecture sir
Thank you for making all my first biochemistry experiments easier.
Masters level ❤:: this is my teacher for my masters class
about F=Fv：Because friction is more complicated, it is usually some empirical formula. In the past, the friction force you were in contact with usually referred to dry friction between solids. If you consider that the object is moving in air or liquid, the measurement will find that the friction force is related to the speed. It is generally considered that at high speed, the friction force is proportional to the square of the speed, and when moving slowly, the friction force is approximately linear with the speed.
More strength Mr andrey ,your lecturers are helpful
Honestly man, how are you so good??
You explain so good, it's just amazing. I understand you better that the same theme in my mother-language :))
No wordss for ua teaching....gets stored in brain
beyond PERFECT
Did you get that A+?
@@AtifKhan-kg3qj Yes I did !!!! Now I'm doing my MSc and imagining I'm watching his videos AGAIN!!!
Thank you so much i just scored a full mark in my final exam because of your lecture God bless you
you do such a great job. thank you XD. I had a few friends who took biochem (lecture and lab) and strongly recommend watching you
I have been following you for years and really I am so grateful, thanks sooooo much :D Your videos are always so clear and complete
you can follow mine
Done!
thank you
I mean he is always slaying ❤❤❤❤❤❤❤
You could teach biology even to an alien! great job buddy!
Your channel is such a wonderful learning resource. Thank you so much!!!
Thank you for the simple explanation and the clear diagrams. I was in a bad need to understand that type of gel electrophoresis.
Fantastic explanation🎉🎉❤
"In just a moment" .... Thank you so much
How is the equation F_f = f*v derived? I know F_f = mu*Fn but not sure how you're getting that derivation.
Loved the way you explain..every point. thanks for explaining it 👍 so well
My uni has to pay for you , Thank you!
one of the beautiful lectures i listened..thanks dear andrey
Drhari Kartha Thanks a ton! :)
your explanation is so clear. thank you.
your lecture is so on point. Thank you so much for being helpful to me!
Lisa Touyon you're welcome Lisa, glad to be of help :)
you are great lecture! It is clear to understand. Thank you so much!
great video! Please continue to make more.
Not be a nerd, but SDS is missing one more carbon in the video (Dodecyl = 12). I only see 11 carbons in the drawing. I appreciate your work, and needed this very much.
You are the best, very helpful clips
That is a very good explanation. Many thanks
The best explanation EVER! Thank you :)
thank you, finally understood physics :D and ofcourse SDS-PAGE... your lectures are great!
+Kathy Pychova you're welcome Kathy :)
Excellent class!!
Best explanation ever, thank you so so much!
I like you, all your videos are so thorough! thank you so much
Thanks Samantha!
You are amazing! Thanks for your explanations
great explanation
what a good presentation. keep it up
you are the best , you should be teaching in Harvard
Great explanation. Little review of physics was helpful
Alex Rytikoff Thanks Alex, always like to use physics when applicable! :-)
AK LECTURESplease show me the link of denatured polyacrylamide gel DNA .
tay zar linn google it.
Very beneficial lecture. Thanks so much....
He is right about the electric field being constant, BUT! Actually the voltage is not constant! In order to maintain a constant electric current, which generates ''almost'' constant velocity, the gel-electrophoresis machine increases the voltage during the process in order to resist the ever increasing impedance values inside the medium (anyone doing gel-electrophoresis can see that). Actually during the migration the voltage more than doubles! Does not matter in the end... Excellent video anyway!
Accidentally today is the birthday of this video 😂
Thanks for a great video. I have one question. How is the force of friction = f*velocity? Isn't frictional coefficient dimensionless so the unit does not come out to be Newtons?
Thank you SO much! Your videos are so helpful!!!
Great explanation!
It occurs to me that the structure formula of SDS written on the white board is incorrect (one "CH2" is missing in the dodecyl fatty acid)...
But otherwise: great explaination!
This was informative and awesome. Thank you!
I think there is a problem, the coefficient of friction must be without dimension, while here you when you multiply f with velocity it does not make sense, would please clarify it???!!!!!
You're the best.
Great video, you are very easy to understand. Does SDS page disrupt disulfide bonds?
+M Abdul You are breaking the sulfide bonds with DTT, BME, or TCEP before you put it in the well.
simply outstanding
your videos always help me, but I only need you to zoom in the board, so tht we can see whts written
Overall good lecture....... 8:20
just what i needed ! thank you very much !! ^_^
adiga202 You're very welcome! :)
Excellent! Well done!
Can anyone please explain Why frictional force is => frictional constant(f) X velocity (v)? Far as i know Frictional force is equal to frictional constant(f) X Normal Force (N)
thank you for this video.
The friction comes from the gel?
can we obtain the separated protein from the gel
brilliant lecture!
f begum thanks! :)
plz i have a question , would you tell me the nature of denaturing take place by SDS on protein which doesnt effect on the conformation of protein
Dope lecture
juzzz in luv wid ur lectures ...(y) !! brilliant !! :)
lovelyy arora thank you! :-)
Hi I'm using potassium sulphate buffer in my sample. can it works with it or does it has to be tris HCl? thank you
Thank you very much ...
totally awesome..thnks very much..
Hands off
amazinnnnng. can i ask a tow question: 1. whats the deference between 6x and 2x loading dye and which i should use ?
2. which voltage is optimal to run one caste of gel, i usually use 65 v. ?
the concentrations of chemicals in the 6x loading buffer is three times as high compared tot the 2x loading buffer. This is the only difference. You should dilute your protein sample with the loading buffer in such a way that you always end up with 1x loading buffer. the 2x loading buffer should be diluted 1:1 with your sample. the 6x should be diluted 1:5.
the concentrations of chemicals in the 6x loading buffer is three times as high compared tot the 2x loading buffer. This is the only difference. You should dilute your protein sample with the loading buffer in such a way that you always end up with 1x loading buffer. the 2x loading buffer should be diluted 1:1 with your sample. the 6x should be diluted 1:5.
Can someone explain how I can use this procedure to determine which membrane proteins are integral vs peripheral?
I always thought the reason ads page was due to size not charge was because the charge to mass ratio for all proteins is the same with SDS.
10 amino acids = every other amino acid gets sds/ = arbitrary net charge of 5. Divide that by 10 = 1/2.
30 amino acid... 15 charge.. 15/30 is 1/2 .Thus all proteins have the same exact charge/mass ratio.
sds add charge uniformly to each and every amino acid so that they can separate based on their charge ratio
Is the charge-to-friction ratio the same thing as the charge-to-mass ratio?
i dont know how can i say u r the best
understood it very well in just the moment :D :)
What is the role of mercaptoethanol???. Plz reply
Farida Chy the presence of mercaptoethanol disrupts the disulfide bridge bond found in the protein when mercaptoethanol is not present the disulfide bond in protiens stay intact hope that was helpful
Mercaptoethanol is a reducing agent that cuts disulfide bonds within proteins in order to disrupt the 3-dimensional shape of proteins, a process that is called "denaturation".
You are the god
Great! Thanks!
I find your explantation of the net force on the proteins in the gel to be problematic. Could you direct me to the source of your explanation so that I can review it?
+Kevin Miles There is no source.
How did you know that the frictional force = frictional coefficient * velocity? I have never seen that equation before.
Thankyou
Thank you!
your the best
Please make a video on Native page
Thanks a lot Sir..
thank you so much for these videos!!!!!! thank you thank you thank you thank you.... oh yeah... THANK YOU :)
Try to explain in many languages, for example in spanish... Nice videos !!
try to teach enlish :)
Thanks a lot sir
Everyone, please answer me, should be running how much mA (current) with mini-gel?
90-120
Thank you so much