11 - SDS-PAGE

In this video, we show how to set up a SDS-PAGE experiment and separate proteins by their molecular weights.
eroglulab.com
/ laberoglu

[@CR-uu1rr]

Congratulations, I have never seen a tutorial as clear, detailed step by step and above all as complete as this one. Truly a lectio magistralis. Thank you, you are super.

[@okyanusya4093]

Thank you, finally i understand total procedure.

[@DEDSE]

Thnx this video change my life for better ❤🙌🏻

[@alialmoussawi1068]

you need to add something between the stacking and seperating so that your gel will be leveled. You can use isopropanol or butanol or simple DW, during the 30 - 60 mins wait time. Read Schagger protocol. Many issues.

[@amymedali]

Very clear procedure. Thanks a lot

[@asma8322]

Thanks for u alot.. Egypt 🇪🇬

[@dilipkulkarni2122]

Excellent. Very clear. Thank you.

[@EramFatima-x6u]

Why did your gel lanes appear blue after running SDS PAGE? I mean when you dismounted the gel, the lanes looked blue and not much difference after staining?

[@manpreetkour7572]

how to purify protein from adherent cells ?

[@Afrin2005]

The music 🔥

[@facundomassolo4663]

Excellent, thank you!

[@leticiadamianodantas8107]

how much marker do you put on the gel?

[@888Marilia]

0.75*

[@DocScoop7]

such a good video

[@NAZIMUDDIN-px4rm]

Honourable Sir./Maam. I feel that it need to add yours video speech sub-title.

[@hitkarshkushwaha2434]

Thanks you so much...

[@danieloulhint7914]

This video is good but the music background is so disturbing

[@remaahmed4894]

I agree it completely distressed me 😢