The Principle of Pulsed Field Gel Electrophoresis (PFGE)

This video provides the best explanation about Pulsed Field Electrophoresis, it helps you to fully understand the principle and the technique.
Watch also Agarose Gel Electrophoresis:
• The Principle of Agaro...

[@Sumit_parihar19]

I must say this is the best video for pulse field gel electrophoresis on RUclips. Not a single indian educator explained this technique as beautifully as you did. Thanks

[@liliancruz4679]

Thank you very much for this video! You're an amazing teacher.

[@reginaamabanu4768]

Excellent explanation , lucid too. thank you

[@jerinjoy5577]

lovely explanation!! thank u so much for this!

[@theshatub962]

good work, so helpful, THANKS!

[@YusufNusretKurkcuoglu]

Dear lady i cannot thank you enough. ❤❤❤❤❤

[@sehrishkhalid126]

The best video of PFGE

[@JuliaSilva-xf1if]

Thank you very much! Excellent explanation

[@mohiniverma9710]

awesome video keep uploading with explanation

[@appppppplecrumble6601]

Ohhhhhhhhhh now you got me reminiscing. i used a PFGE set up when i worked in cancer research years ago. The hope was to find a set up that could be scaled up to workout if patient A, B or C needed the *full dose* of cancer treatment or hopefully slightly less.
i have black hair if i waned to dye it red i’d have to use one hell of a lot of red dye (probably have to bleach it first then dye it red). Someone with blonde hair would not need the extra step & would need less dye. So if thats true about hair colour, we are all different amounts then it surely must be true about DNA.
i was trying to explain all this to a friend so glad to come across this video.
Thou not sure you quite described how fiddly the bloody thing was, especially in summer. So i have lovingly made my little tiny squares with human dna. i then zap the poor little squares of gel with x-rays, causing the double strand breaks. Then the little squares of jelly get their own back as using a biology fume hood with your hands up inside & then in that position you had to get each jelly square balanced on the tip of a scapel, sometimes trim it CAREFULLY and place it into the well.
Sounds easy until you think (especially in summer) you are trying to fit two bits of jelly together, neatly & accurately You have to be cardful put it in with no mushing it in or breaking the plug. The more you mush it up you are creating more double strand breaks. There were times when....... lol. i just 😶😶😶. Txs for the work you are doing here i might nerd out & have a look around.
Is couple of videos showing the lab equipment, my baby, but you certainly explained it best.
Thanks for a trip down memory lane.

[@debjyotipaul23101988]

Excellent presentation and well-explained! Thank you!

[@xinjin7661]

That's very helpful for me,thank u very much~

[@maheshkhalate438]

Nice video ....easy to understand thank you for sharing this video to us

[@syedakanwalzehrazaidi7265]

great video and great explanation

[@bhargaviminchu6137]

The best explanation.. ver easy to understand

[@mariasoledadorellano4312]

excellently explained !

[@GracielleFreitas]

nossa, amei o vídeo, só lendo o artigo não deu pra sacar. ótima explicação!! Obrigada

[@noufalssab297]

Brilliant brilliant explanation thank youuu

[@rizwanarather7808]

Perfectly cleared

[@tracyanncampbell7367]

Nice video. Thank you

[@jaydeepdumadiya2464]

Thank you...
God bless you

[@NicoLamannaR]

Great video! Really easy to follow. However, I have a question concerning the switching time parameter: When I want to perform this technique, the system asks me to input two values (for example: initial time 10 sec; final time 30 sec). What exactly mean those two values??

[@sahinsaikh5004]

Outstanding your teach

[@ashirt2100]

Thank you so much...

[@jayaogirala1824]

Thank you very much very helpful vedio thanku

[@a.m.g1268]

Great mam 👍

[@girmaalemu1792]

Well explained! tnx

[@Jai_Jagannath-ji]

Nice video 👍

[@susmitamondal4795]

thank you.

[@syed-wd8ib]

very informative

[@mukuldev1244]

simplified and throughly explained...
great work..
Thank You

[@biomedicalandbiologicalsci4989]

you are welcome :)

[@khouloudameursoualem4029]

Thanks💗

[@amandeeprana4920]

Wow amazing mam , pls make video on NMR

[@npainnogame07]

thanks

[@wilmayuangu9386]

Thank you so it helps me to understand much better 😍
Keep making vedio

[@nandilindasheila4812]

Thank u

[@sindhug1322]

VERY CLEAR EXPLANATION, THANK YOU!

[@biomedicalandbiologicalsci4989]

Thank you ... stay tuned :)

[@bountoysibounhoueng4118]

very use full video thank you for your sharing

[@bountoysibounhoueng4118]

thanks for your good video

[@alyaaalmery7939]

You really helped me
Thank you so much 👍

[@yijingwang7308]

Thank you for your video. But I am confused why do not we just extract and elongate DNA samples outside and put them into the wells? So we do not need to use several currents to elongate them.

[@isaacyoung1868]

how does PFGE change when doing it in a capillary? because i think that in capillary, there is only one direction possible for the electrical field right?

[@vikrambhaskar7976]

love your accent:)

[@garicobiang8729]

I like ur accent 🥰

[@oaendrilaghosh3179]

Mam please make the video on ftclp

[@nermurugarren270]

That was useful, thank you!

[@ansonrey9234]

I know Im kinda randomly asking but do anyone know of a good site to watch newly released series online ?

[@kamrynfinn1248]

@Anson Rey I watch on Flixzone. You can find it by googling :)

[@zakaimakai1367]

@Anson Rey Try Flixzone. Just google for it =)

[@atlasnikolai5398]

@Anson Rey Try Flixzone. You can find it by googling =)

[@kendallbrayden212]

@Kamryn Finn yea, I've been using FlixZone for since march myself :D

[@yijingwang7308]

Other questions are about the parameter setting:
1. I think a larger angle should be better for resolution since the route can be longer. I do not understand why you said the smaller angle is better.
2. You mentioned that the time and voltage should be the same for turning the right and left direction. But as the DNA elongates, its moving speed will also change. So although you use the same voltage and time, it may still cannot be straight. How to deal with that?

[@namithasureshvanparambil4317]

Thank you for the awesome explanation...can you make video on SELDI procedure too?

[@noureddinehalla5267]

Yhank you.....It was well explained

[@biomedicalandbiologicalsci4989]

you are welcome :)

[@thyagohermylly]

hi ...amazing video really helped. how can i get in touch with you. ?

[@mxmajewski]

Great video, very useful review

[@biomedicalandbiologicalsci4989]

Thank you for your comment ... stay tuned :)

[@biology9214]

🙏🙏🙏

[@Imanheaaaartsings]

Can you make a video on column chromatography, circular dichroism, fluorescence spectroscopy and x-ray crystallography please?

[@biomedicalandbiologicalsci4989]

I am working on the chromatography videos right now, then I will try to work on the other topics ... thank you for your comments :)

[@mohammeddambous6246]

I think when I listen to your voice how you look like

[@oaendrilaghosh3179]

Fast protein liquid chromatography

[@valerieting3115]

Please stop tongue smacking and your 'errr'. Quite irritating to hear.

[@MegaMie77]

Or or or, look here.
I have an idea.
Listen to this, YOU can skip this video and avoid being irritated leaving everyone else to learn in peace.
What a concept?!

[@tanvirahmed-rr7vt]

@@MegaMie77 agreed

[@Bashariat1]

thanks