The Principle of Agarose Gel Electrophoresis, a full explanatory video

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  • Опубликовано: 30 июл 2024
  • This video is a full and clear explanation about the principle and the applications of agarose gel electrophoresis.
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Комментарии • 104

  • @romelsoyza4160
    @romelsoyza4160 6 лет назад +3

    A complete and very easy to understand explanation (like the videos for Flow Cytometry and FACS) on the principles behind the technique.

  • @AyazSamo
    @AyazSamo 5 лет назад +2

    She is great a great teacher! Respects from Sindh!

  • @gilyasungi4572
    @gilyasungi4572 5 лет назад +3

    Well explained! Good explanations linked with real life applications.

  • @ladushky1
    @ladushky1 3 года назад +1

    Thank you! we have never add the colour right into the gel tho.. but we do add it to the samples we are running on the gel.

  • @RohitPant04
    @RohitPant04 3 года назад +1

    Concise & informative! Good job. 🙏

  • @mohammedholba2498
    @mohammedholba2498 6 лет назад +1

    One of the best youtube chanel l ever seen. :)

  • @tiaxi6779
    @tiaxi6779 5 лет назад +1

    very informative video, explained in detail for the reason of each step.

  • @walrus4248
    @walrus4248 4 года назад

    Will see if you already did capillary electrophoresis, this was such a fine explanation thanks.

  • @mohammedal-hammadi5085
    @mohammedal-hammadi5085 5 лет назад +2

    Wow, this video is so clear and useful, besides you are awesome, thank you so much

  • @user-ff9vu8ic7b
    @user-ff9vu8ic7b Год назад

    بجد احسن حد شرح الموضوع دة بالتوفيق❤

  • @walrus4248
    @walrus4248 4 года назад

    Fantastic, I learned a lot and I didn't know anything about the subject.

  • @ridzwanfauzi975
    @ridzwanfauzi975 4 года назад +1

    great video!!really helped me in my test..thank you so much

  • @mohanndri64
    @mohanndri64 6 лет назад

    Such a good and simply way of explanation thank you

  • @joan-b-empire576
    @joan-b-empire576 Год назад

    Love from Cameroon 🇨🇲 thank you

  • @muhammadjaber2272
    @muhammadjaber2272 6 лет назад +1

    Your voice like best for presenting and I have get many from your channel

  • @sukanyahembrom6213
    @sukanyahembrom6213 7 лет назад +1

    very well explained.... thanx alot for making such video

  • @worldaviation4k
    @worldaviation4k 4 года назад +4

    so this isn't used to be able to look at the dna letters? Thanks I've had trouble trying to find the videos where you take the dna and look at the actual proteins. maybe i will find it soon :)

  • @poetrylover5561
    @poetrylover5561 3 года назад

    You've nicely explained the topic. Many thanks for sharing ♥️

  • @maggiejameel6725
    @maggiejameel6725 5 лет назад +1

    Very well explained, you are an awesome teacher. I SUBSCRIBED

  • @stephaniehurtado4096
    @stephaniehurtado4096 2 года назад

    Late to the upload but I’ve watch almost all your video and they all been so helpful:) ofc ill like and subscribe!

  • @dstan16224
    @dstan16224 4 года назад +1

    Thank you very much ma'am.It really helped a lot.

  • @ciao_abhi
    @ciao_abhi 6 лет назад +1

    This helped me so much for my mcat test

  • @srigayathri5980
    @srigayathri5980 2 года назад +1

    Thank you so muck mam.Because of u i can able to understand the concept now mam.

  • @Animelover7948
    @Animelover7948 2 года назад

    Very very well explained...very helpful....people out there can try watching this vid

  • @krisantinimarkam3396
    @krisantinimarkam3396 Год назад

    Very well explained and easy to understand. Thank you

  • @bahahos7426
    @bahahos7426 4 года назад

    Thank u very much i love all your videos

  • @sanskrutiraj8051
    @sanskrutiraj8051 11 месяцев назад

    This video is very helpful for me. Thank you u explained in easy way 🙂

  • @VarshiiiPrem
    @VarshiiiPrem 5 лет назад +1

    A great teacher..... Thanks a lot... Tomorrow is my exam... Very useful

  • @Amar45611
    @Amar45611 3 года назад

    Thank you Ma'am.. It's a great video! ❤

  • @abdulmunafisalisuumar7016
    @abdulmunafisalisuumar7016 6 месяцев назад

    thank you so much maam, for your wonderful explanation of Principle of Agarose gel electrophoresis.

  • @wonderakpese1901
    @wonderakpese1901 Год назад +1

    Concept well explained. Thank you

  • @rahmanebrahimzadegan7198
    @rahmanebrahimzadegan7198 2 года назад

    You teach excellent. Thank you.

  • @Gbemi78
    @Gbemi78 5 лет назад

    your explanation is second to none.

  • @trona2612
    @trona2612 5 лет назад +2

    Hi Ma'am, thank you very much for such a good,short & crisp but very much informative video on agarose gel electrophoresis.... I've a question on the differential mobility profile of nicked & linear DNA molecules of same size; why do linear DNA molecule migrates ahead of nicked one? I'll will be grateful to hear from you. Thank you!

  • @Min-gh1jr
    @Min-gh1jr 6 лет назад +1

    Hi this video is great! I was wondering if the thickness of the band would suggest any properties about the nucleic acid strand, i.e there are more nucleic acid fragments of that size?

  • @girlschannel11
    @girlschannel11 6 лет назад

    Really good explanation !!!!

  • @qamarhennawi9137
    @qamarhennawi9137 2 года назад

    You are the best, no one like you

  • @Katherine-mf9wz
    @Katherine-mf9wz 2 месяца назад

    Nice job!

  • @bharathkumart6097
    @bharathkumart6097 5 лет назад

    Thank you very much for this video ma'am

  • @tanimaferdous50
    @tanimaferdous50 6 лет назад +1

    Very very good explanation

  • @setarehsohail5422
    @setarehsohail5422 2 года назад

    It was a very nice lecture!

  • @pritichristian2673
    @pritichristian2673 2 года назад

    Thanks. Very clear explanation

  • @iliyamohd8154
    @iliyamohd8154 3 года назад

    Very good presentation

  • @yennguyen-tp7ce
    @yennguyen-tp7ce 6 лет назад +1

    everything is just so clear, thanks

    • @ikhinerawlings124
      @ikhinerawlings124 Год назад

      Well explained. Thanks. Can talk on chromatography in details

  • @halafr5861
    @halafr5861 5 лет назад +2

    You are the best thank you from my heart 💜

  • @jyothinandakumar6190
    @jyothinandakumar6190 Год назад

    Very well explained

  • @fasilutubechannel6697
    @fasilutubechannel6697 2 года назад +1

    thanks i hope another video release in this related video

  • @robinkhan6419
    @robinkhan6419 2 года назад

    Well presentation

  • @felixjohnpaulbarqueros6675
    @felixjohnpaulbarqueros6675 5 лет назад

    Hi how can I determine the effectiveness if i will alternative coloring dye for the loading buffer

  • @stevemorton3078
    @stevemorton3078 4 года назад

    Are PCR and then AGE being done in an automated fashion on a single sample in a miniature electrochemical device, such as one that can only be used once ?

  • @insanhabib4497
    @insanhabib4497 4 года назад

    Thanks a lot, ma'am.

  • @samanthabautista1655
    @samanthabautista1655 5 лет назад

    Hi im just wondering which buffer to use when your stain is acidic? Because according to a journal ive read that stain that we are about to use when expsed to basic enviroment it can affect the staining capability in a bad way? So which buffers to use??? Anyone can answer? It would help me a lot for our research please

  • @tinyikonkuna1479
    @tinyikonkuna1479 2 года назад

    😊😊😊😊Love it here plz make more videos on biology concepts especially 4 varsity students

  • @shailendrayadav5799
    @shailendrayadav5799 4 года назад

    You are great teacher mam

  • @sahewa100
    @sahewa100 6 лет назад +6

    Very well explained.... thanx alot for making such video. This helped a lot in my thesis research. Please continue to teach us :))

  • @thnxm8
    @thnxm8 2 года назад

    Thanks a lot

  • @geeswags6172
    @geeswags6172 2 года назад

    Informative

  • @soheilaazam799
    @soheilaazam799 4 года назад +1

    You are the best molec teacher, who I have ever seen!!! Well done 👍😊 and thanks for the Videos. What is your name by the way???

  • @yamaatomayi4315
    @yamaatomayi4315 6 лет назад +4

    mem i like your explanation, i love to hear u r voice again and again u r a great talent .. and i really love..

    • @briang1310
      @briang1310 5 лет назад +1

      why do indian people always says mem or ma'am

    • @SandeepKumar-dd3ie
      @SandeepKumar-dd3ie 4 года назад

      @@briang1310 coz it is taught and its logical as well to respect the efforts

    • @gabecalderon2043
      @gabecalderon2043 2 года назад

      @@SandeepKumar-dd3ie learn respect then

  • @jiaflair
    @jiaflair 2 года назад

    THANKYOU 💞

  • @Az-xr5yx
    @Az-xr5yx 5 лет назад

    Thanx thats was helpfull

  • @saraabdulaziz3764
    @saraabdulaziz3764 2 года назад

    Very informative🥰

  • @dikshashreedevi4568
    @dikshashreedevi4568 5 лет назад

    please make some more videos .good explanation

  • @krishnanandh7999
    @krishnanandh7999 3 года назад

    Supr class. Thank you🤩🤩

  • @joyeke6340
    @joyeke6340 2 года назад

    Thank you

  • @claudiaaurie8161
    @claudiaaurie8161 6 лет назад +1

    Hi! I just found you and I have been watching your videos. They are amazing! I was wondering (this may be a silly question) but how do you know which restriction enzymes to use for example for paternity testing? does it matter which to use as long as all DNA samples are treated the same? thank you! look forward to watching all your videos--Claudia

    • @biomedicalandbiologicalsci4989
      @biomedicalandbiologicalsci4989  6 лет назад

      Hei .. thank you for your comment ... as you said, you should treat all the samples with the same restriction enzyme .. and experimentation will show us which is the best restriction enzyme to use :)

    • @sidratulmuntaha1687
      @sidratulmuntaha1687 5 лет назад

      @@biomedicalandbiologicalsci4989 Can we search it out from the literature which endonuclease to be used?

  • @Milahh.
    @Milahh. 7 лет назад +1

    Thx, very useful well done

  • @lincolnkarim1
    @lincolnkarim1 3 года назад

    This is not my field at all so please excuse my ignorance; Since DNA is a negative molecule (3:48), does that mean it is an ion?

  • @nextagro
    @nextagro 3 года назад

    Not sure if the comments are still answered, but... Why do we need a PLATINUM wire electrode in the electrophoresis setup? What exactly will happen if one replaces that electrode with an ordinary Copper wire?

  • @jaswantnegi4879
    @jaswantnegi4879 5 лет назад

    Please upload some videos about difference between RNA and DNA isolation and separation techniques. Do tell about Difference in their agarose gel concentration and gel separating chamber, where we use horizontal or vertical gel chambers...

  • @ranaaamiraamir3437
    @ranaaamiraamir3437 5 лет назад

    Brilliant

  • @kuldeepdinkar4918
    @kuldeepdinkar4918 6 лет назад

    What is the use of nylon fiber?

  • @MaryGraceBayot
    @MaryGraceBayot Год назад

    Thanks

  • @emanwanli7740
    @emanwanli7740 3 года назад

    I have a question .. when migrating the genomic DNA .. shouldn't multiple bandits appear, each expressing a single chromosome content .. since chromosomes carry DNA of very different sizes, especially if we are talking about humans, for example

  • @jthomas0007
    @jthomas0007 2 года назад

    Hi ma'am, could you please talk about PCR and it's various types?

  • @devyaniitware2428
    @devyaniitware2428 6 лет назад

    What are amplicons?

  • @stevanstankovic8021
    @stevanstankovic8021 4 года назад +2

    Great video, thanks!
    I have a question concerning visualisation: If after the gel we have the blue bands with the loadind dye, why do we need to add Ethidium bromide to make it visible under UV? Isn't the information we have from the blue bands sufficient? Or is it maybe, that the blue bands we see after we ran the gel is maybe almost only the dye itself, as it leaves the DNA (or RNA) and just sinks faster to the + side, so that our actual DNA (or RNA) is located a bit above the dye - and that is why we need to check it under UV, as it would be "invisibly" located above the dye? Really can't find any answer to this! Thanks again! (:

    • @ladushky1
      @ladushky1 3 года назад +1

      We either add GelRed right into the gel or we use ethidium bromide later for visualization; both are carcinogens but we would not use both at the same time.

  • @statusboys4036
    @statusboys4036 2 года назад +1

    God give me an opportunity to spend 15 mins of my life time in a precious way...

  • @urmilahussainpiya6735
    @urmilahussainpiya6735 5 лет назад

    please make a vedio why Taq DNA polymerase is thermostable

  • @drgaikwadsir7270
    @drgaikwadsir7270 5 лет назад

    Nice mam

  • @drgaikwadsir7270
    @drgaikwadsir7270 5 лет назад

    Make some videos related with r dDNA technology

  • @Art-cq1zy
    @Art-cq1zy 2 года назад

    Im confused. Why does the solution not evaporate in the microwave?

  • @redeemerbadagbor1673
    @redeemerbadagbor1673 3 месяца назад +1

    you do all :salut

  • @ItsKhan0011
    @ItsKhan0011 4 месяца назад

    ❤❤❤

  • @youcare6415
    @youcare6415 7 лет назад +17

    Very informative. I guess it is SYBR Green not cyber green ;)

    • @anirbanhait5998
      @anirbanhait5998 2 года назад

      Ma'am have pronounced rightly.

    • @SLNmin
      @SLNmin Год назад

      It is SYBR, pronounced as cyber but correct spelling is SYBR.

  • @wycliffenyandika9017
    @wycliffenyandika9017 Год назад

    like it

  • @sukantamandal8106
    @sukantamandal8106 4 года назад

    Replication

  • @jsvclubdeciencia6283
    @jsvclubdeciencia6283 3 года назад

    In silico: ruclips.net/video/BkTRYMjyatA/видео.html

  • @tıbhendese
    @tıbhendese 3 года назад

    I don't think anything in nature and universe is junk or useless

  • @ladushky1
    @ladushky1 3 года назад

    Sorry, but there is no such thing as DNA junk sequence anymore.

  • @samanthabautista1655
    @samanthabautista1655 5 лет назад

    Hi im just wondering which buffer to use when your stain is acidic? Because according to a journal ive read that stain that we are about to use when expsed to basic enviroment it can affect the staining capability in a bad way? So which buffers to use??? Anyone can answer? It would help me a lot for our research please

  • @nagendrag7562
    @nagendrag7562 3 года назад

    explained very nicely

  • @abeerm9786
    @abeerm9786 4 года назад

    Thank you