so this isn't used to be able to look at the dna letters? Thanks I've had trouble trying to find the videos where you take the dna and look at the actual proteins. maybe i will find it soon :)
Hi Ma'am, thank you very much for such a good,short & crisp but very much informative video on agarose gel electrophoresis.... I've a question on the differential mobility profile of nicked & linear DNA molecules of same size; why do linear DNA molecule migrates ahead of nicked one? I'll will be grateful to hear from you. Thank you!
Hi this video is great! I was wondering if the thickness of the band would suggest any properties about the nucleic acid strand, i.e there are more nucleic acid fragments of that size?
Are PCR and then AGE being done in an automated fashion on a single sample in a miniature electrochemical device, such as one that can only be used once ?
Hi im just wondering which buffer to use when your stain is acidic? Because according to a journal ive read that stain that we are about to use when expsed to basic enviroment it can affect the staining capability in a bad way? So which buffers to use??? Anyone can answer? It would help me a lot for our research please
Hi! I just found you and I have been watching your videos. They are amazing! I was wondering (this may be a silly question) but how do you know which restriction enzymes to use for example for paternity testing? does it matter which to use as long as all DNA samples are treated the same? thank you! look forward to watching all your videos--Claudia
Hei .. thank you for your comment ... as you said, you should treat all the samples with the same restriction enzyme .. and experimentation will show us which is the best restriction enzyme to use :)
Not sure if the comments are still answered, but... Why do we need a PLATINUM wire electrode in the electrophoresis setup? What exactly will happen if one replaces that electrode with an ordinary Copper wire?
Please upload some videos about difference between RNA and DNA isolation and separation techniques. Do tell about Difference in their agarose gel concentration and gel separating chamber, where we use horizontal or vertical gel chambers...
I have a question .. when migrating the genomic DNA .. shouldn't multiple bandits appear, each expressing a single chromosome content .. since chromosomes carry DNA of very different sizes, especially if we are talking about humans, for example
Great video, thanks! I have a question concerning visualisation: If after the gel we have the blue bands with the loadind dye, why do we need to add Ethidium bromide to make it visible under UV? Isn't the information we have from the blue bands sufficient? Or is it maybe, that the blue bands we see after we ran the gel is maybe almost only the dye itself, as it leaves the DNA (or RNA) and just sinks faster to the + side, so that our actual DNA (or RNA) is located a bit above the dye - and that is why we need to check it under UV, as it would be "invisibly" located above the dye? Really can't find any answer to this! Thanks again! (:
We either add GelRed right into the gel or we use ethidium bromide later for visualization; both are carcinogens but we would not use both at the same time.
Hi im just wondering which buffer to use when your stain is acidic? Because according to a journal ive read that stain that we are about to use when expsed to basic enviroment it can affect the staining capability in a bad way? So which buffers to use??? Anyone can answer? It would help me a lot for our research please
A complete and very easy to understand explanation (like the videos for Flow Cytometry and FACS) on the principles behind the technique.
She is great a great teacher! Respects from Sindh!
Well explained! Good explanations linked with real life applications.
Thank you! we have never add the colour right into the gel tho.. but we do add it to the samples we are running on the gel.
Concise & informative! Good job. 🙏
One of the best youtube chanel l ever seen. :)
very informative video, explained in detail for the reason of each step.
Will see if you already did capillary electrophoresis, this was such a fine explanation thanks.
Wow, this video is so clear and useful, besides you are awesome, thank you so much
بجد احسن حد شرح الموضوع دة بالتوفيق❤
Fantastic, I learned a lot and I didn't know anything about the subject.
great video!!really helped me in my test..thank you so much
Such a good and simply way of explanation thank you
Love from Cameroon 🇨🇲 thank you
Your voice like best for presenting and I have get many from your channel
very well explained.... thanx alot for making such video
so this isn't used to be able to look at the dna letters? Thanks I've had trouble trying to find the videos where you take the dna and look at the actual proteins. maybe i will find it soon :)
You've nicely explained the topic. Many thanks for sharing ♥️
Very well explained, you are an awesome teacher. I SUBSCRIBED
Late to the upload but I’ve watch almost all your video and they all been so helpful:) ofc ill like and subscribe!
Thank you very much ma'am.It really helped a lot.
This helped me so much for my mcat test
Thank you so muck mam.Because of u i can able to understand the concept now mam.
Very very well explained...very helpful....people out there can try watching this vid
Very well explained and easy to understand. Thank you
Thank u very much i love all your videos
This video is very helpful for me. Thank you u explained in easy way 🙂
A great teacher..... Thanks a lot... Tomorrow is my exam... Very useful
Thank you Ma'am.. It's a great video! ❤
thank you so much maam, for your wonderful explanation of Principle of Agarose gel electrophoresis.
Concept well explained. Thank you
You teach excellent. Thank you.
your explanation is second to none.
Hi Ma'am, thank you very much for such a good,short & crisp but very much informative video on agarose gel electrophoresis.... I've a question on the differential mobility profile of nicked & linear DNA molecules of same size; why do linear DNA molecule migrates ahead of nicked one? I'll will be grateful to hear from you. Thank you!
Hi this video is great! I was wondering if the thickness of the band would suggest any properties about the nucleic acid strand, i.e there are more nucleic acid fragments of that size?
Really good explanation !!!!
You are the best, no one like you
Nice job!
Thank you very much for this video ma'am
Very very good explanation
It was a very nice lecture!
Thanks. Very clear explanation
Very good presentation
everything is just so clear, thanks
Well explained. Thanks. Can talk on chromatography in details
You are the best thank you from my heart 💜
Very well explained
thanks i hope another video release in this related video
Well presentation
Hi how can I determine the effectiveness if i will alternative coloring dye for the loading buffer
Are PCR and then AGE being done in an automated fashion on a single sample in a miniature electrochemical device, such as one that can only be used once ?
Thanks a lot, ma'am.
Hi im just wondering which buffer to use when your stain is acidic? Because according to a journal ive read that stain that we are about to use when expsed to basic enviroment it can affect the staining capability in a bad way? So which buffers to use??? Anyone can answer? It would help me a lot for our research please
😊😊😊😊Love it here plz make more videos on biology concepts especially 4 varsity students
You are great teacher mam
Very well explained.... thanx alot for making such video. This helped a lot in my thesis research. Please continue to teach us :))
Thank you for your support :)
Thanks a lot
Informative
You are the best molec teacher, who I have ever seen!!! Well done 👍😊 and thanks for the Videos. What is your name by the way???
mem i like your explanation, i love to hear u r voice again and again u r a great talent .. and i really love..
why do indian people always says mem or ma'am
@@briang1310 coz it is taught and its logical as well to respect the efforts
@@SandeepKumar-dd3ie learn respect then
THANKYOU 💞
Thanx thats was helpfull
Very informative🥰
please make some more videos .good explanation
Supr class. Thank you🤩🤩
Thank you
Hi! I just found you and I have been watching your videos. They are amazing! I was wondering (this may be a silly question) but how do you know which restriction enzymes to use for example for paternity testing? does it matter which to use as long as all DNA samples are treated the same? thank you! look forward to watching all your videos--Claudia
Hei .. thank you for your comment ... as you said, you should treat all the samples with the same restriction enzyme .. and experimentation will show us which is the best restriction enzyme to use :)
@@biomedicalandbiologicalsci4989 Can we search it out from the literature which endonuclease to be used?
Thx, very useful well done
Thank you for your comment ...
This is not my field at all so please excuse my ignorance; Since DNA is a negative molecule (3:48), does that mean it is an ion?
Not sure if the comments are still answered, but... Why do we need a PLATINUM wire electrode in the electrophoresis setup? What exactly will happen if one replaces that electrode with an ordinary Copper wire?
Please upload some videos about difference between RNA and DNA isolation and separation techniques. Do tell about Difference in their agarose gel concentration and gel separating chamber, where we use horizontal or vertical gel chambers...
Brilliant
What is the use of nylon fiber?
Thanks
I have a question .. when migrating the genomic DNA .. shouldn't multiple bandits appear, each expressing a single chromosome content .. since chromosomes carry DNA of very different sizes, especially if we are talking about humans, for example
Hi ma'am, could you please talk about PCR and it's various types?
What are amplicons?
Great video, thanks!
I have a question concerning visualisation: If after the gel we have the blue bands with the loadind dye, why do we need to add Ethidium bromide to make it visible under UV? Isn't the information we have from the blue bands sufficient? Or is it maybe, that the blue bands we see after we ran the gel is maybe almost only the dye itself, as it leaves the DNA (or RNA) and just sinks faster to the + side, so that our actual DNA (or RNA) is located a bit above the dye - and that is why we need to check it under UV, as it would be "invisibly" located above the dye? Really can't find any answer to this! Thanks again! (:
We either add GelRed right into the gel or we use ethidium bromide later for visualization; both are carcinogens but we would not use both at the same time.
God give me an opportunity to spend 15 mins of my life time in a precious way...
please make a vedio why Taq DNA polymerase is thermostable
Nice mam
Make some videos related with r dDNA technology
Im confused. Why does the solution not evaporate in the microwave?
you do all :salut
❤❤❤
Very informative. I guess it is SYBR Green not cyber green ;)
Ma'am have pronounced rightly.
It is SYBR, pronounced as cyber but correct spelling is SYBR.
like it
Replication
In silico: ruclips.net/video/BkTRYMjyatA/видео.html
I don't think anything in nature and universe is junk or useless
Sorry, but there is no such thing as DNA junk sequence anymore.
Hi im just wondering which buffer to use when your stain is acidic? Because according to a journal ive read that stain that we are about to use when expsed to basic enviroment it can affect the staining capability in a bad way? So which buffers to use??? Anyone can answer? It would help me a lot for our research please
explained very nicely
Thank you