Western Blotting (Immunoblotting) : Principle and Complete Procedure Explained ! (FL-Immuno/81)

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  • Опубликовано: 9 янв 2025

Комментарии • 167

  • @siddhipawar8697
    @siddhipawar8697 6 лет назад +88

    Awesome video. All the steps are clearly explained and it it seriously better than all the other videos out there. Keep up the good work. Hope to see more videos in future ❤️

  • @ikeriokwuehifestus8382
    @ikeriokwuehifestus8382 4 года назад +13

    There couldn't be a better and well-explained video than this!

  • @getitdone0010
    @getitdone0010 9 месяцев назад +2

    No idea who is behind this channel....THANK YOU SO MUCHHHHHH

  • @davalcom
    @davalcom 3 года назад +5

    What a explanation, man!!!!! Requirement of every single step was really crucial.... Keep it up

  • @zoief4088
    @zoief4088 6 лет назад +8

    Wonderful presentation and explanation!
    I love the way you teach. You clear all our doubts in just 5-10 minute video.
    Thank you so much.

    • @FrankLectures
      @FrankLectures  6 лет назад +3

      Glad to know this. Thank you for your feedback.

  • @aswathyashish5529
    @aswathyashish5529 3 года назад +2

    Finally a video that explains what Western blotting is in the simplest manner. Thank you so much 🙏🏼 and hats off to your great effort !

  • @D1trox
    @D1trox 4 года назад +1

    OMG! this video is better than 3h of 3 courses of my University *o*

  • @uzochukwuebube359
    @uzochukwuebube359 3 года назад +1

    Thank you so so much, I love you for this, the explanation is so detailed and simple to understand thank you so much and God bless you

  • @Justgirlmustache
    @Justgirlmustache 4 года назад +8

    I do wish this made sense in real life. Currently doing western blots and constantly get bands that are not the probe of interest. There is not alot of information as to the problems with antibodies e.g. non-specific binding, housekeeping protein binding and secondary's antibodies binding non-specifically

  • @mangoinjuice
    @mangoinjuice 6 лет назад +1

    Thank you! It helps me understand more easier. Though I am not good at English, the video was really thankful to me!

  • @ahmadal-harbi9987
    @ahmadal-harbi9987 3 года назад

    No body ever explain it as I saw it here, very impressive 👏

  • @harshitdwivedicrazydancerb2488
    @harshitdwivedicrazydancerb2488 4 года назад +1

    A very great video, all the concept of Western Blotting is clear now. Thanks for this a lot. ☺☺☺☺☺☺☺☺☺☺☺☺☺☺

  • @abirev1585
    @abirev1585 5 месяцев назад

    Thanks a lot for such a clear explanation, keep up the great work.

  • @learnislam5353
    @learnislam5353 4 года назад

    Very nice lecture....this video give lots of information & clearly explanation for that topic... Thank you so much

  • @edithmalivaachale577
    @edithmalivaachale577 2 года назад +1

    This is so clear. Thank you for the detailed explanation.

  • @61syedaaliya65
    @61syedaaliya65 3 года назад +1

    Best video I've ever watched so perfectly and beautifully explained...each and every point was clear... Thank you so much 👍

  • @satyasravani9533
    @satyasravani9533 5 лет назад

    Super video each and every step was explained clearly tq so much this is a best method for Easy learning

  • @mesmerizingnature1612
    @mesmerizingnature1612 3 года назад

    Very eliquently explained
    Thank you so much

  • @acidbase7866
    @acidbase7866 Год назад

    Excellent video for understanding the concept of western blotting

  • @veronicalam8606
    @veronicalam8606 4 года назад +4

    Thank you for making this video, it helps me to clear up some concepts. I wonder how it can be used for detection of allergy using blood simple. Please keep going.

  • @priyaanjimusic7806
    @priyaanjimusic7806 5 лет назад +1

    I love ur way of teaching

  • @nandeeshanp4721
    @nandeeshanp4721 6 лет назад

    one of the good video to know about western blotting in easy way

  • @khizraabid8964
    @khizraabid8964 3 года назад

    U have teach it in a very smart way !

  • @wafaqa4236
    @wafaqa4236 5 лет назад +1

    Very useful. . May allah reward u👍👍❤

  • @nennietandel9154
    @nennietandel9154 4 года назад

    greatly explained, in very much detail. Loved it! will help a lot during exam.:)

  • @riddhimanchatterjee4195
    @riddhimanchatterjee4195 2 года назад

    Greatest lecture on the topic

  • @mohammedal-hammadi5085
    @mohammedal-hammadi5085 5 лет назад +3

    It's awesome video seriously, thank you so much 😊

  • @khaoulabouteraa8838
    @khaoulabouteraa8838 3 года назад

    the best channel eveer🖤😍😍

  • @refad333
    @refad333 6 лет назад +1

    Great .,. Description and voice is awesome

  • @arathiunnikrishnan6901
    @arathiunnikrishnan6901 5 лет назад +1

    Great explanations.. very useful. Thank you

  • @maheswaramrammurthy5258
    @maheswaramrammurthy5258 6 лет назад +2

    Outstanding animation video .anybody can understand

    • @FrankLectures
      @FrankLectures  6 лет назад

      Glad to know this. Thank you for your feedback.

  • @dilkisilva3202
    @dilkisilva3202 2 месяца назад

    Well explained , awsome work❤❤

  • @manishajogdand8490
    @manishajogdand8490 5 лет назад

    Very nice step by step explanation... Thnk u.. Keep up such good work and upload more video relateed to B pharmacy

  • @puspamkeshri7730
    @puspamkeshri7730 2 года назад

    Great video, nicely explained each step!!

  • @rimpa98528
    @rimpa98528 5 лет назад

    Thank you madam. This video help me a lot. Thank you so much.

  • @hamismussa428
    @hamismussa428 Год назад

    Big up from Tanzania

  • @harryhaimoud726
    @harryhaimoud726 2 года назад +1

    I smashed that subscribe thing immediately .

  • @snehap2608
    @snehap2608 4 года назад

    Thanks alot for this video.. video is so informative..♥️♥️

  • @livinghope8039
    @livinghope8039 3 года назад

    Thank you for the wonderful explanations! I liked + subscribed it!!

  • @afokujabi7256
    @afokujabi7256 3 года назад

    Thank you...very very informative and useful

  • @V1Veeeee
    @V1Veeeee 5 месяцев назад

    Since English is not my first language, I just can’t easily understand this accent. But good video!❤

  • @morvaridrahnama5743
    @morvaridrahnama5743 3 года назад

    Very clear and well explained. thank you.

  • @farwachoudry5947
    @farwachoudry5947 4 года назад

    Thank you soo much for this video. Helped alot

  • @ouuuaburger2093
    @ouuuaburger2093 4 года назад

    Professors=🤯
    RUclips channel=😳

  • @arshida98
    @arshida98 8 месяцев назад

    Nice explanation 👏👍👍👍

  • @ednadanielacastillomartine9297
    @ednadanielacastillomartine9297 4 года назад +1

    This is amazing, really helped a lot. Thanks! (:

  • @shmonikindalo5947
    @shmonikindalo5947 Год назад

    Thank you so much for this video❤

  • @tiaraannisa1869
    @tiaraannisa1869 2 года назад

    very helpful, thank you for an awesome video!

  • @arfatulwahidbhat5658
    @arfatulwahidbhat5658 4 года назад

    Thanks ma'am.. It was really helpful... :)

  • @tunahanyapici3201
    @tunahanyapici3201 2 года назад

    this is so clear, thank you so much..

  • @ankitayadav1123
    @ankitayadav1123 5 лет назад +1

    Really. Awesome thank you so much 😘

  • @athirahshafiee7579
    @athirahshafiee7579 2 года назад

    great explanation! thank you so much!

  • @sutopopranoto7750
    @sutopopranoto7750 5 лет назад +2

    thanks, for detail and clearly explained

  • @mariasastradjaja2318
    @mariasastradjaja2318 4 года назад +2

    wuahhh this is so clear :) Thanks!

  • @KeepRowing247
    @KeepRowing247 5 лет назад +1

    Excellent video!

  • @egbuwalofemi9836
    @egbuwalofemi9836 3 года назад

    This ia an awesome video, thank you so much

  • @حوراءحيدر-م2ذ
    @حوراءحيدر-م2ذ 4 года назад

    Awesome and helpfull video thank you so much

  • @mosesalo
    @mosesalo Год назад

    Very good lecturer

  • @wishvasandaruvan2291
    @wishvasandaruvan2291 Месяц назад

    Awesome video.Thank You

  • @neetakushwaha1206
    @neetakushwaha1206 5 лет назад +2

    Thank you for this video ma'am.....

  • @samerqib8358
    @samerqib8358 6 лет назад +3

    I would suggest you to make video lectures on PCR

    • @FrankLectures
      @FrankLectures  6 лет назад

      Already uploaded the video on our second channel. Link - ruclips.net/video/mbhOcshEDJM/видео.html

  • @TechwithKamran
    @TechwithKamran 3 года назад

    Very well explained👍

  • @drsd2010
    @drsd2010 3 года назад

    Superb video 👌

  • @scienceofyourlifesoul
    @scienceofyourlifesoul 3 года назад

    👏well explained✌️

  • @samerqib8358
    @samerqib8358 6 лет назад +2

    Great video I would suggest you to make video lectures on Pcr

    • @FrankLectures
      @FrankLectures  6 лет назад

      You can watch video lecture on PCR on my second channel - HOTS Biology.

  • @maryamyousaf700
    @maryamyousaf700 4 года назад

    It is fantastic video indeed

  • @Utub_r
    @Utub_r Год назад

    Thank you so much, did u uploaded eliza test explanation

  • @nasromahamudduwane1941
    @nasromahamudduwane1941 2 года назад

    Well explained 👏

  • @SHARINGCARINGLOVING
    @SHARINGCARINGLOVING 3 года назад +1

    Good work ❤️

  • @menadkhelil1930
    @menadkhelil1930 5 лет назад +1

    Well explained thank you !

  • @sabaakhtar4680
    @sabaakhtar4680 5 лет назад +2

    Very well explained. Can we have a video on cell cycle, mitosis and meiosis?

    • @FrankLectures
      @FrankLectures  5 лет назад +2

      Thanks. Cell cycle and mitosis video lectures are already available on this channel. Just type the topic and "frank lectures" in the search box.

  • @alinahussain92
    @alinahussain92 6 лет назад +1

    Very helpful...thnx 👏

  • @lemonwood272
    @lemonwood272 Год назад

    beautiful. thank you so much.

  • @BaljitKaur-zj9tm
    @BaljitKaur-zj9tm 5 лет назад

    well explained, to the point

  • @shreyagowdaabuddingdoctor7898
    @shreyagowdaabuddingdoctor7898 6 лет назад +1

    Really helped me to prepare for neet and aiims

  • @naziaparween92
    @naziaparween92 4 года назад

    Thank u very much ma'm ....

  • @shwetaawasthi3222
    @shwetaawasthi3222 2 года назад

    Amazing👌

  • @amitsinghbhadoriya6318
    @amitsinghbhadoriya6318 Год назад +1

    Thanks 😊

  • @hiroshiro7443
    @hiroshiro7443 6 лет назад +5

    Thanks, I'm a little confused in the detection part.
    When I use a different substrate will it produce a different color???

  • @beryllabrinth3884
    @beryllabrinth3884 2 года назад

    Hello, do we stain before blocking step to visualize the membrane?

  • @Antymills424
    @Antymills424 5 лет назад +1

    Very helpful!

  • @negarolfati36
    @negarolfati36 2 года назад +1

    The link for SDS-PAGE is not working

  • @maryamyousaf700
    @maryamyousaf700 4 года назад +1

    The detecting step is not overlapping with ELISA??????if it is then what is the difference between ELISA and WB????????

  • @myraalina4934
    @myraalina4934 6 лет назад +2

    Thank u 😇😇😇😇

  • @suhaibaziz4597
    @suhaibaziz4597 2 года назад

    Thank you 🌹🌹🌹

  • @Aman_Bhosale
    @Aman_Bhosale 5 лет назад +1

    Nice video ...

  • @moumitamallick5426
    @moumitamallick5426 4 года назад

    great video

  • @szemelele2580
    @szemelele2580 3 года назад

    It is brilliant !!!

  • @saileeshahane2810
    @saileeshahane2810 5 лет назад +1

    Can you please make a video for competitive Elisa i need it Thank you🙌

  • @bhatrumanarumana6215
    @bhatrumanarumana6215 6 лет назад +1

    Please make videos of southern and Northern bloting also

  • @STUDENTSDEN
    @STUDENTSDEN 17 дней назад

    understood easily

  • @alwaysambitious
    @alwaysambitious 5 лет назад +1

    Wonderful!

  • @JassyWassySquared
    @JassyWassySquared 2 года назад

    Fantastic!

  • @shantanibeditamohanty1286
    @shantanibeditamohanty1286 4 года назад

    Thank you very much maam

  • @aurelysmarie7417
    @aurelysmarie7417 4 года назад

    Why is the gel on the cathode part and the membrane on the anode?

  • @sonalimhatre8588
    @sonalimhatre8588 5 лет назад +1

    Thank you!!

  • @hussain223
    @hussain223 6 лет назад +1

    A huge thanks

  • @sumayyarani6828
    @sumayyarani6828 4 года назад

    JazakAllaH 👌

  • @gauravp5088
    @gauravp5088 6 лет назад +1

    Can the label also be a radiolabel thats picked up by an xray?

    • @FrankLectures
      @FrankLectures  5 лет назад +1

      Earlier radiolabels were used. This practice is now discontinued because of new and efficient labelling methods.

  • @SakshaPrakash
    @SakshaPrakash 2 года назад

    Useful vedio