Primary and Secondary Antibodies (FL-Immuno/69)

[@marcianoaugusto1]

I've been study for my exam trough these videos. They are amazing. Good work, Frank Lectures. Thank you very much!!!

[@FrankLectures]

Glad to know this. Thank you for your valuable feedback.

[@tanjamist]

Aside from the computer voice. This was very well done. Thank you.

[@yaoxiao4142]

The great content made me neglect that computer voice. Thank you for this work.

[@Motaki666]

@Frank Lectures thank you for the video. Question - When a product is labelled as Goat anti-mouse Ab for example, does this mean that it has been raised in goats and binds to mouse Ab? And by default are these always secondary Abs?

[@hamidkiangaikani]

Awesome. thanks very much ♥

[@omarellazdy4022]

It is great to know the difference , yet I would like to know why labelling primary antibodies reduces their immunoreactivity ??

[@klaudiaczapla843]

Why is it important to ensure that you remove all unbound secondary antibody before adding the substrate?

[@amitghosh8723]

Very useful video with nice description. ...

[@FrankLectures]

Thank you for your valuable feedback.

[@bernatufekci1622]

Wonderful. Thank you very much..

[@tanvirahmed-rr7vt]

You are a GEM. Mam

[@shwetaprasad4375]

Thank you so much 😊 it's very helpful.

[@MiauxCatterie]

thank you so much for this video explanation.

[@maryamsohail8892]

How labelling primary Ab affects it's immunoreactivity? Kindly explain it

[@baharsarikamis6888]

So clear! Thank you :)

[@Uk12336]

Crystal clear lecture

[@blackout2829]

Could you just explain how these antigens or antibodies are immobilized at the bottom of microtiter? Anyway, videos are great.

[@FrankLectures]

+Black Out thank you for appreciating these videos.
The immobilization of antigens and antibodies in micro titer wells depends on the chemical interactions between the molecule and the surface of the well.
To explain this in detail, another video lecture is required. But for your query, you can read this PDF explaining immobilization principles. Click this link www.google.co.in/url?sa=t&source=web&rct=j&url=www.cultek.com/inf/otros/soluciones/ELISA_superficie.pdf&ved=0ahUKEwjy4sPU7LHYAhVEOI8KHbd7D-kQFggmMAA&usg=AOvVaw2eU_a7vtzjbUorKm32Gogd I hope this is useful to you.
Thanks.

[@blackout2829]

Frank Lectures Yes, it's helpful. Once again, thank you.

[@FrankLectures]

you're welcome :)

[@Lucydog18]

Great video

[@diaroychowdhury8815]

Lovely lovely

[@emilyb5832]

this was so helpful!

[@jagrutimote7772]

Can u explain sandwich test for immunofluorese

[@dr.arunsundarmohanasundara7632]

Awesome

[@carlosbarraza9150]

GREAT VIDEO!

[@FrankLectures]

Thanks

[@arifhussainkhand8300]

❤❤

[@goharrehman7710]

very good

[@ryanhimawanwibowo6014]

good explanation, thank you :)

[@FrankLectures]

Thanks

[@Rahul-xv1fw]

Thanks a bunch

[@bhagyashrishinde6735]

👍👍👍

[@goharrehman7710]

the principal of the test is understood IHC kit test please

[@susanakoch9776]

Sorry, would live to listen to the lecture but I hate the computer voice, lack of tone which helps to assimilate the message

[@FrankLectures]

It's ok . I respect your preferences. I have and had some limitations regarding voiceover for my video lectures. But I haven't compromized the quality of content in these video lectures. Thanks.

[@priyankasarangi411]

Too good

[@FrankLectures]

Thanks

[@jadumonigogoi845]

secondary antibody also exist!!

[@FrankLectures]

Yes

[@venkybly]

Tq

[@goharrehman7710]

IHC I learn IhC and stain

[@ryanhcy3317]

ANTEYEBAWDY

[@rohitjagtap1492]

1st comment😎😎😎

[@FrankLectures]

+rohit jagtap thank you. But you haven't told whether this video is useful or not. :)

[@rohitjagtap1492]

Frank Lectures
Tooo good

[@FrankLectures]

+rohit jagtap thank you for your feedback.

[@tinox12]

omg i hate those computer voices so much

[@rawan_hamamreh]

The voice is soooo baaad