Shomu You are really genious. Your way of explanation is very nice and easy. I am also teacher and doctorate but you way of teaching is quite better than me. Respect for you
I have watched all ur videos during my graduation, as m in my last semester. To be very honest, Sir your lectures help me a ton in making my foundation in microbiology. Keep doing great works. May God bless you. Always greateful 👏👏👏
I have my interview for associate scientist in 2 hours and I’m listening to your videos..these are so helpful and I just want to say thank you for pouring your knowledge here. May God bless you with everything you desire❤
Yeah Shomu I agree with all these comments, you are essentially a God among Gods.. Quite frankly, you are a savior.. i've been watching your videos ever since I began my biotechnology degree. Whenever I need extra explanation on any concept, I am sure to find a video of you doing the expert explanations.. Thank you!
Shomu this is an excellent tutorial on western blotting. I just read the methods section of a research paper on western blotting and didn't understand much but after watching ur video I can confidently say I understood everything in that method section of the journal now! Thank u!
Sir thank you so much for western blotting explanation. I have exam on 17oct n it will help me.meri dua h k Ap aise hi sabki help Karein or Apka shomu biology khub tarakki Kare Inshallah 🤗😊
Because of you sir my concepts are very clear about the topics that I listened ur lectures.. you are really an awsm teacher..May Allah bless you with more Knowledge And Success..🙂
Thank you so much for your lecture. I finially understand the material. I think You are really talanted at teaching! I hope you keep on posting these helpful videos😊😊
Hello sir, i have been wathing your videos since my 11th ...12th and now I'm in my 3rd yr biotech. Your efforts are truly ...amazing and hatss off to you for the efforts u put to make such good quality explaination over quantity. ✨💥😁
Thank you so much!! You are truly talented when it comes to teaching!! Everything is explained in such an understandable and basic manner! Keep it up😊😊😊
Thanks alot sir , my exam is on 14th of May and u helped me alot in this topic ...anyways you help me everytime..hehe ..you are a genius sir..may god bless you ...❤️
Hello, Apka bohot bohot shukriya Jo ap etne mhnat se sabkeliye ye information as in the form of lect. You're presented. Pata nahi apne sabko achese deep level Tak samjh aaye common language mai sab chizo ka dhan rakha, I'm from Maharashtra, Mujhe Bengali to Ati nahi hai wese, Par ye apke liye,,, #AtiSundarama 😊😇😇😇
Awesome vid. God someone needs to invent one-step simple ways to do these things. All makes sense. Simple enough. Hell of a lot of steps / time / work. C'mon, it's like we're in the stone age.
I have a few questions as stated below: 1) why did we attach the negative electrode to the SDS-gel if it already contains a negative component i.e. the sulphate that helped to denature the protein? 2) If multiple Ab binds to a specific protein,is there a chance to get improper result? 3) When did we add the Luminol?
A key part of the lecture he missed is the role of SDS and that West blotting detects Amino acid (AA) sequence for proteins (other blots detect nucleic acid sequence). AA have a Verity of charges. On treating the denatured protein (I.e AAs) with SDS, the SDS coats the surface of the unfolded AAs to give the protein a net negative charge. Hence the proteins have a -ve charge and move towards the positive electrode attached to the membrane. You need both -ve and +be fields to run electrophoresis. 2. Antibodies are usually highly specific to antigens, but in this case,some stray away and bind to the membrane (Hence "blocking" is necessary or you get false +ve results) 3. The secondary antibody which binds to the primary antibody (an anti-immunoglobulin) is conjugated with an enzyme eg Horse radish peroxidase (HRP). HRP catalyzes the degradation of the substrate luminol. As Luminol degrades, it emits light the position of which is detectable on x-ray film
Shomu , you are really a good guy and thanks for your videos , i am subscribing you from Iraq .. I have only very simple note about this video .. dear brother when you draw the membrane after blotting , the transferred bands should be resemble to bands you did draw on gel after electropheresis , bcz this step only for transferring , but at the last step ( probing ) this will not happen bcz only target protein will appear . Many thanks
Sir you said when current passed from gel (-ve charged) to membrane (+ve charged) by by conventional we know current move from " +ve to -ve". Then how this imprinting is formed? And why the gel has to be negatively charged?
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Shomu You are really genious.
Your way of explanation is very nice and easy.
I am also teacher and doctorate but you way of teaching is quite better than me.
Respect for you
Thank you so much for appreciating my efforts
I agree completely. I was so confused reading textbook and don't need all this indepth knowledge but he explains it so nicely
thanks alot prof.shomu may GOD go before you on everything you expect, coz you have a good lecture
Great job sir..solving our problems in an easiest way.. jzakillah
Sir, do you provide any notes
Dear Shomu, I am a teacher and regularly listen to your videos to help me make students understand in an easy way. Ur excellent. God bless u.
Thank you very much for appreciating my efforts
You can directly ask them to see this. Because I'm student and he is our savior .
I have watched all ur videos during my graduation, as m in my last semester. To be very honest, Sir your lectures help me a ton in making my foundation in microbiology. Keep doing great works. May God bless you. Always greateful 👏👏👏
Thank you so much for appreciating my efforts
If your videos didnt exist i wouldnt even pass my bitechnology paper ,thank you so much sir your skills have got me inspired.Great Respect to You !!!
You're welcome
I have my interview for associate scientist in 2 hours and I’m listening to your videos..these are so helpful and I just want to say thank you for pouring your knowledge here. May God bless you with everything you desire❤
Thank you so much for appreciating my efforts. All the best
Heyy did you get the job!
Sir u dont disappoint me when i want study any topic of biology i found each and every topic of ur lectures🙂
Thank you so much for appreciating my efforts
But I can't find autoradiography topic
I want to learn from him 😭😭
I am saying now u will become definitely a great person tomorrow. This society should have a good teacher like u☺️
Thank you so much for appreciating my efforts
Yeah Shomu I agree with all these comments, you are essentially a God among Gods.. Quite frankly, you are a savior.. i've been watching your videos ever since I began my biotechnology degree. Whenever I need extra explanation on any concept, I am sure to find a video of you doing the expert explanations.. Thank you!
Thank you so much for appreciating my efforts. Glad to hear that you're getting benefit from my lectures
Shomu this is an excellent tutorial on western blotting. I just read the methods section of a research paper on western blotting and didn't understand much but after watching ur video I can confidently say I understood everything in that method section of the journal now! Thank u!
Glad to hear that you are getting benefit from my videos
This channel is a saviour when there is no tym..🔥💯
You're welcome
I had my immunology exams sir I did it very well .. thanks to you. Love your teaching ❤️❤️❤️
Sir thank you so much for western blotting explanation. I have exam on 17oct n it will help me.meri dua h k Ap aise hi sabki help Karein or Apka shomu biology khub tarakki Kare Inshallah 🤗😊
Thank you so much for appreciating my efforts
Because of you sir my concepts are very clear about the topics that I listened ur lectures.. you are really an awsm teacher..May Allah bless you with more Knowledge And Success..🙂
Thank you. Glad you liked my lectures
you're studying things in really easy ways, I always recommend your videos to my friends
aftr an hour I have sem exm..
Tq 4 ur class .. full support
You're welcome
excellent sir...I am verymuch impressed by the way you teach
+Pritha Ghosh thank you. Glad you liked my lectures
You are my favorite teacher, sir . thank you so much Sir.. I just love your way of teaching..
No one can explain with such a beutiful way u explain. Thanku very much
You're welcome
Seriously best teacher for this on the internet THANK YOU
Thank you so much for appreciating my efforts
Though my preparation was not good, you saved my exam today. You made this topics so easier. Thanks a lot
Glad to hear that you are getting benefit from the videos
Thank you so much for your lecture. I finially understand the material. I think You are really talanted at teaching! I hope you keep on posting these helpful videos😊😊
saved my exam .. thank you so much sir. I am a huge fan of yours.
Hello sir, i have been wathing your videos since my 11th ...12th and now I'm in my 3rd yr biotech. Your efforts are truly ...amazing and hatss off to you for the efforts u put to make such good quality explaination over quantity. ✨💥😁
Thank you so much for appreciating my efforts. Glad to hear that you're getting benefit from my lectures
Thank you. Whenever I am curious about something, your videos help me with my curiosities clearly.
God bless you Sir, I have an exam in two weeks, and this information was really helpful🙏🙏
Glad to hear that you're getting benefit from my lectures
Thank you so much!! You are truly talented when it comes to teaching!! Everything is explained in such an understandable and basic manner! Keep it up😊😊😊
Shomu you too Sabi book. You explain everything so succinctly.
Well done.
Thank you so much for appreciating my efforts
After watching it i m excited to perform it in lab. Thank u sir u r the best teacher
You're welcome. Glad to hear that you're getting benefit from my lectures. Please subscribe and share
Sir your teaching style is awesome...l understand everything which you used to deliver.
Thank you so much for appreciating my efforts
you are really good deliver the lectures Alhumdullilah my all blotting are cleared now ...Thank u soo much
Thank you so much for appreciating my efforts. Stay tuned
Shomu, our biology professor asked us to take notes on this video for homework! you rock!!:))
Thank you so much for appreciating my efforts
Yesterday i watch ur video about southern bloting nd today i help my frndz .they easily understand the topic.
Glad to hear
A very didactic and informative tutorials. You are doing great!
+adesina omoloye thank you. Glad it helped
You are the best teacher ever 😊😊😊😊 all the very best sir ❤️.
Glad to hear that you're getting benefit from my lectures
SDS-PAGE DOES NOT separate molecules by charge, only by polypeptide chain length (size) 5:04
Kitna samjhne ki koshish ki maine par nahi hua par tum to kamal nikle yaar....😎 Owsome ❤️
Thank you
You are truly a gem.
Keep shining sir
Thank you so much for appreciating my efforts
Thanks alot sir , my exam is on 14th of May and u helped me alot in this topic ...anyways you help me everytime..hehe ..you are a genius sir..may god bless you ...❤️
Thank you. Glad you liked my lectures
THANK YOU VERY MUCH, SHOMU!!! YOUR TEACHING IS AWESOME. MAY GOD BLESS YOU.
Thank you so much for appreciating my efforts
big round of aplause for that comprehensive explanation
Thank you
i have an exam tommorow and this really helped thank you 🙏🏼
You're welcome
You are legend, Sir. what an explanation.....superb...
Thank you so much for appreciating my efforts
Thank uh so much sir.... Lots of love and support from Maharashtra 🙏❤
Thank you so much for appreciating my efforts
excellent, being a chemist, still I could understand very nicely. Thanks for your clear explanation
Thank you
The best explanation of western blotting ! thank you
You're welcome
tomorrow is my exam... this is helping me alot... thankyou so much sir
+Krystal R you're welcome.
My interest in botany is only because of you sir thank you so much
You're welcome. Glad to hear that you're getting benefit from my lectures
I'm not going to finish my college without you
Glad to hear that you're getting benefit from my lectures. Please subscribe and share
Love your lectures, its a refresher for me after a long period.
Hello,
Apka bohot bohot shukriya Jo ap etne mhnat se sabkeliye ye information as in the form of lect. You're presented.
Pata nahi apne sabko achese deep level Tak samjh aaye common language mai sab chizo ka dhan rakha,
I'm from Maharashtra,
Mujhe Bengali to Ati nahi hai wese,
Par ye apke liye,,,
#AtiSundarama
😊😇😇😇
This is so helpful! The concept behind every step is explained clearly, brilliant video!
+Riya Paul thank you. I am so glad to hear that
Best teacher ever!
Thank you so much for appreciating my efforts
Explained far better than my professor. Thank you 👍
Thank you. Glad you liked my lectures
This is the best video I have found on this topic, thank you so much!!!!🤗
thanks to you sir... I'm preparing for my exam from ur lectures..
tomorrow is my exam.
already studied rflp rapd aflp southern and Northern blotting.
+siddhesh pundekar all the very best for the exam. Hope you will do good
This week we executed this and it is remarkable.
It is remarkable in deed
You are an amazing science teacher!!! Thanks for making it easy to understand!!! You are very knowledgeable!! Merci beaucoup!! Keep it up!!!
Glad to hear that you are getting benefit from the videos
Sir thank you so much for this ... this helped me learn this topic for my final exam.
Glad to hear that you're getting benefit from my lectures
Thank sooo soo much sir really have no words to explain for your hardwork keep it up, good will bless you forever and ever and ever❤❤❤❤❤❤❤🤗😊🙏
Thank you so much for appreciating my efforts
Sir ur explanation is really awsm... u r my real hero... ty sir
Thank you. Glad you liked my lectures. Please subscribe and share
Thank u sir i had written what u said in all blotting topics. Thank you very much for for the knowledge u given to me
Thank you. Glad you liked my lectures
Super raa...😂😂
supper....students of life sciences will be gratefull to u....love and respest to you...
the best explanation so far. wish i could give multiple thumps up.
Thank you for appreciating my efforts
You are the best teacher!
Thank you so much for appreciating my efforts
Thank you so much sir 🙏. Allah bless you with health and wealth and happiness
Thank you so much for appreciating my efforts
Thank you sir.....I appreciate the way you teach and with lot of enthusiasm ❤️
You're welcome. Glad to hear that you're getting benefit from my lectures
Thank u sir for urs traching while comparing my lecturer u teaching is super
You're welcome. Glad to hear that you're getting benefit from my lectures. Please subscribe and share
K sir
Thank god to give me a chance to watch this video, it does help me a lot!
Thank You very much Suman Dada for all those helpful videos..
God bless you and your success reach the sky..
Ur explaining very well kept it up . Lots of love
Thank you
great job👍 it is the simpliest way to remember the procedure. thanks
You're welcome
Thank you sir I have MSc Zoology exam on 29th of this month....You helped me a lot to clear my doubts
thanks for video , i have exam today , if this comes in exam i will bless you by heart still thank you
All the best
Great informative explanation, greets from Egypt bro.
I can't ask for better explanation... Thank you man 👏
I love the way you teach ❤
Thank you
Oh Sir another concept clearing video❤
Thank you so much for appreciating my efforts
you are really an excellent teacher .. feeling grateful to find your page
U r such a great preacher...man...hats off to u👍keep d pace
Your explanation was great.
Thank you
That was a very simple and perfect explanation..Thank you very much ☺
Good Bless u sirr..Ur teaching method is so Good❤
Thank you. Glad you liked my lectures. Please subscribe and share
Thanku Thanks alot.. it is very useful for me n helpful in my bachelor's ppr..
+huma qidwai glad to know that
Thank you sir... Ur lectures r too good and easy to understand
Excellent sir and superb method of ur teaching
Thank you very much for appreciating my efforts
Your work is highly understandable I appreciate it
thanks
You're welcome. Glad to hear that you're getting benefit from my lectures
Tqqq sm bro ... doing really great work. ...👍👍 God bless you..❤️
You're welcome
Perfect explanation ❤
Thank you
More much good explanation sir.
You're welcome
Awesome vid. God someone needs to invent one-step simple ways to do these things. All makes sense. Simple enough. Hell of a lot of steps / time / work. C'mon, it's like we're in the stone age.
Not really
Sir what a explanation !😍
Thank you so much for appreciating my efforts
really to good for the beginner as well as experienced graduate and postgraduate
Thank you
you r awesome....your videos are so easy to understand...thankyou so much
Informative.. Good explanation.. Thank you
You're welcome
Wonderful explanation Sir.
Thank you
I have a few questions as stated below:
1) why did we attach the negative electrode to the SDS-gel if it already contains a negative component i.e. the sulphate that helped to denature the protein?
2) If multiple Ab binds to a specific protein,is there a chance to get improper result?
3) When did we add the Luminol?
A key part of the lecture he missed is the role of SDS and that West blotting detects Amino acid (AA) sequence for proteins (other blots detect nucleic acid sequence).
AA have a Verity of charges. On treating the denatured protein (I.e AAs) with SDS, the SDS coats the surface of the unfolded AAs to give the protein a net negative charge. Hence the proteins have a -ve charge and move towards the positive electrode attached to the membrane. You need both -ve and +be fields to run electrophoresis.
2. Antibodies are usually highly specific to antigens, but in this case,some stray away and bind to the membrane (Hence "blocking" is necessary or you get false +ve results)
3. The secondary antibody which binds to the primary antibody (an anti-immunoglobulin) is conjugated with an enzyme eg Horse radish peroxidase (HRP).
HRP catalyzes the degradation of the substrate luminol. As Luminol degrades, it emits light the position of which is detectable on x-ray film
Shomu , you are really a good guy and thanks for your videos , i am subscribing you from Iraq .. I have only very simple note about this video .. dear brother when you draw the membrane after blotting , the transferred bands should be resemble to bands you did draw on gel after electropheresis , bcz this step only for transferring , but at the last step ( probing ) this will not happen bcz only target protein will appear . Many thanks
+Karam Dawood yes. I know. That is the thing shown in the video
You're a such amazing lecturer!
Sir you said when current passed from gel (-ve charged) to membrane (+ve charged) by by conventional we know current move from " +ve to -ve". Then how this imprinting is formed? And why the gel has to be negatively charged?
DNA is negatively charged and moves to the positive electrode.
No words sir only namaskar 🙏🙏🙏🙏🙏🙏🙏