ELISA test procedure | what is elisa assay?
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- Опубликовано: 2 авг 2021
- Elisa test procedure - This lecture explains about the elisa test principle and the elisa test procedure. The elisa test is to identify specific antigen or antibody to detect the presence of bacterial infection. Elisa test can detect the presence of antigen or antibody in patient specimen that help us to detect bacterial infection. Stay tuned to know answers to the following questions -
What is elisa test?
Elisa test procedure?
Elisa test principle?
Elisa test classification?
How elisa test is done?
What is elisa assay?
Elisa test is also known as the enzyme linked immunosorbent assay.
Stay tuned to know the elisa test explained in this video lecture.
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Thank you for watching the video lecture on elisa test procedure and the principle of elisa test.
Legends are watching this video one day before exam 😅 btw....this video is very informative easy to understand helped me a lot. Thanks Shomu's biology
You're welcome. Glad to hear that you're getting benefit from my lectures
Mera kal exam hain😂
@@simadassaikia8588 okay carry on 😄 I think you're from Assam 🤔
@@zakiyasohana3037 Yes I am form Assam
@@simadassaikia8588 From which institute?
Hello!
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Again, your explanation is so vivid, real in the sense that even a layman person could easily comprehend unlike others who will be just reading a downloaded ppt from google. continue.. your good work and may God be with you.
You r the warrior, our saviour. I become familiar with a topic of which I don't even know a single word and able to write it completely in exam. Thank you my teacher. ❤❤
Glad you liked it
Thankyou so much sir... Tomorrow is my exam and I wasn't able to understand a single thing about this topic but you made it easier. Thankyou so much again. Your new subscriber 🌼🌼
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@@shomusbiologyofficial sir today I was able to write on it perfectly.Thankyou again sir. This video helped a lot and I recommended this to my friends too.
Same case with me
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Maine +3 apke video dekh kar khattam ki hai ,msc first year main hoon aur mujhe ye bhi pata hai k app ke vdo hinn mujhe pass kar sakti hai ,thank you sir 😁😁😁you are excellent 👍🏻👍🏻👍🏻👍🏻 learning from odisha
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Thank you so much for appreciating my efforts. I am Honored. Stay tuned for more help. All the best for future career
Thank you so much Shomu Ji- you are such a blessing!
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Very well explained! I have seen many videos on elisa before but you explained in best way .. and finall I understood the concept of this topic. Thanx alot
You're welcome. Glad you like it when
My favourite channel where topic is explained perfectly in a simplified way...
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Thankyou sir. I am ug student of Biotechnology and am doing my internship in medical College and continuing this topic. You explained very well sir I have understood the whole only in one hour. Thankyou plz keep up posting.
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You are indeed the best teacher!!!!!!!
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Now i have a confidence that i will be pass....Thank you sir 🙏
All the best for future career
Thank you. Very nicely explained the complicated technique in such a simple way. Best Regards
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Such a wonderful explanation sir
Thank you very much Sir
✨✨
Favourite teacher ever
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Thank you sir. for making my ongoing MSC. So easy for me _💕
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Thank you so much for your videos. I have just passed my biochemistry exam using your videos and i got top marks. You are the Best ❤️
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Grandeee
I have wached many videos but i have not got the concept, like always you make my doubts crystal clear thak you so much Sir.
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I can clear my masters because of these types of videos by you sir.. thank you so much
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You surpassed uni doctors by ur amazing explanation. Thank so much for ur efforts.
Can you please download more videos for data analysis please 🙏🏻
Thank you
This Topic became so clear because of your lecture🙏🏻🙏🏻
Thank u so much sir ☺️
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Thank you so much, Sir
Sir, your way of teaching is just awesome 🙏
Thank you
Thankyou so much sir, I got very clear cut differences of all types of ELISA Test, very well explaination
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Thank you sir, it was so clear and understandable..❤❤❤❤
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Great job done... Keep moving forward...
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Thankyou so much... watching it 3 hours before paper/
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I m an undergraduate student of Biochemistry and of course so thankful to you for making our courses so easy❤
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Salam from Bangladesh ❤
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much of love for you my dear brother
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This video is very informative nd easy to understand
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Such a Lovely lecture 🤗
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very very well explained sir
thank u so much sir 👌👌🙏🙏🙏
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Sir you are a fabulous teacher.. Sir Please make a video on enzyme technologies too.
Sure. Thank you
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Excellently explained ✨
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Thank you
Excellent video for elisa
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@@shomusbiologyofficial I was wandering here and there to make myself understand the actual concept, went through a lot of videos but this one cleared my concept,, keep spreading quality knowledge 🤍🤍
Thank you so much sir 🙏
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Hello sir can u pls create another video on flow cytometry ....this video is very helpful for me and for many students ...you are truly doing great job for students who have lots of doubts and with your educational video all doubts easily cleared
Thank you so much for appreciating my efforts. Coming soon
Thank you sir you are amazing 🤩
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Superb explanation sir....👏
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Omg is this video for me👀Tomorrow i have immunology Exam🙂🌚... Plz Bless be My sir@shomus biology
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@@shomusbiologyofficial it's my pleasure sir... Really you doing great work♥️😍♥️
Thanks SB...
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Hello. Can you please do a video or notes on how to calculate the reagents of indirect ELISA. Please!
Okay I will try
Awesome 👏✨
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Thank you sir
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nice video
If primary antibody has the affinity to bind to the well, then antigen vil also have the affinity to bind to the well n there is a chance of binding of secondary antibody to the antigen n gives us a colour in the end.
Primary antibody don't have with
Affinity to bind well but it is fixed in well
Elisa plate or well is coated with blocking agent that prevents further binding or unnecessary binding
Thank you sir ☺☺
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Sir thank you so much
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Amazing teaching sir! Sir can u tell that ELISA is specific to only proteins test or it can also be used to detect any microorganism? Im not associated with this just asking for gk!
Sir please make a video on ESBL and MBL
PLEASE DO VIDEO ON GMO Testing, and why it important to test GMO in food sample
Okay
Thanks 🙏
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@@shomusbiologyofficial i want lecture notes of this topic
Hi shomu.hi shomu ❤️
Much respect from pakistan Sir.
Thank you so much for appreciating my efforts
Can U explain Geneious De novo assembly and Contig mapping ??
during washing the unbound substance is remove from the whells😊
sir wanted to know if the intensity of colour formed has anything to do with intensity of the disease?
Sir... basic peptide of tissue .... in thic topic no any one video in youtube....so please made it
Sir ,I hardly request you to make one video on Immunological assay .
Ok
Sir how to design the primary ab against ag x ?
Sir please do make a video on how to prepare for GATE biotechnology 2021🙏🙏🙏
Okay
Worth watching 🙌
Thank you
Very well explained. I have one question why we skip washing step in direct Elisa because we are using enzyme linked antibody and in the next step have to add substrate , substrate because product by enzyme or if antibody is not linked to particular analyte but we have enzyme linked antibody and substrate in solution so is there any chance of colour production. I am so confused please explain
Watch the other varieties of elisa lectures
Sir , you are a fabulous teacher....
Sir , please, can u make a video on the working of the China vaccine "Sinopharm".. please sir...
Thank you so much for appreciating my efforts
Sir kindly explain application.
Why will secondary antibody bind with antigen x when ag and ab follow lock and key mechanism ? when it has already bound with primary ab it shouldn’t bind with the other ab.
well, your question is very good but my point of view the answer is that although Ag Ab bind together so secondary Ab also follow the same lock and key model it does not follow any other method to bind with Ag, lock and key model does not say that one Ag Ab phenomenon always bind, does not matter how many Ag or Ab bind. Hope u got it
Sir thnkx a lot of u 🙏🏻🙏🏻🌹🌹🌹🌹
Love from...odisha 👏🏻👏🏻
You're welcome
Can I write this on exam paper??? As it is....?
In the case of sandwich ELISA, we have a secondary antibody that can interact with a particular region of primary antibody then why after second washing and adding substrate it will not show color?
Cause the substrate not individually added it is linked to the sec antibody
Why serum is preferred than blood in ELISA test
Hi sir. Gd morning! Is secondary antibody which has linked with enzyme is similar to the primary antibody?
No. Secondary antibody binds to primary antibody and it has enzyme linked to it
Sir as you had told antibody- antibody don't bind to each other on time 12:56 in video. but in previous video of yours for topic Western blotting you told that antibody binds to antibody Fc region and shows color in solution.
But the Fc region is already attached to well or may be both antibodies are not similar to bind each other ... As per my understanding i think that might be the reason ab doesn't bind to ab
Can someone tell me what peroxide is it ?
What about capture Antibody ?
Does serum contain antigens!??
❤❤❤❤
Please make video on ABO DESCREPANCY IN DETAIL IN HINDI PLEASE DEAR SIR ❤❤
Okay
Sir which test is best for sars cov2 antibody CMIA OR ELISA
Both are good
Where is the enzyme to add?
❤❤
Thank you
Sir ..,we want optical immuno assay vedio
Okay
Sir i can't find elisa or ria incase of hormone assay which is in my syllabus...one day left for my exam...please reply here that incase of hormone how can l use the word hormone in substitute for antigen antibody... Please sir give me reply... Nobody can tell me this answer... I am waiting sir🙏🏻🙏🏻🙏🏻🙏🏻🙏🏻
I am glad
If you use wrong buffer or simple water than also kit will show accurate results
Please make a video on gal operon
Sure
In plants ELISA is different?
sir please explain easy methods first and then go for complex methods,
All methods are already explained
Why only detects protein ?
Sir please make a video of RIA
Sure
👌👌👌👌👌
Thank you
The Direct Elisa and Sandwich Elisa is same...Correct it
Your qualifications my dear
Msc in Microbiology
I requested you k mery exams hyn plz made video on types of biosensors and you highlighted my comment but don't made.... disappointed
It's not that simple. I have videos lined up.
Necesito los subtítulos :(
🙏🙏🙏🙏
Thank you