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HPLC chromatography
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- Опубликовано: 3 июл 2016
- HPLC chromatography lecture - This lecture explains about the HPLC chromatography technique in a nutshell by Suman Bhattacharjee. HPLC is performed to separate organic and biological compounds using solid stationary phase.
High Performance Liquid Chromatography (HPLC) is a form of column chromatography that pumps a sample mixture or analyte in a solvent which is known as the mobile phase at high pressure through a column with chromatographic packing material known as stationary phase. The sample is carried by a moving carrier gas stream of helium or nitrogen. HPLC has the ability to separate, and identify compounds that are present in any sample that can be dissolved in a liquid in trace concentrations as low as parts per trillion. Because of this versatility, HPLC is used in a variety of industrial and scientific applications, such as pharmaceutical, environmental, forensics, and chemicals.
Sample retention time will vary depending on the interaction between the stationary phase, the molecules being analyzed, and the solvent, or solvents used. As the sample passes through the column it interacts between the two phases at different rate, primarily due to different polarities in the analytes. Analytes that have the least amount of interaction with the stationary phase or the most amount of interaction with the mobile phase will exit the column faster.
This lecture explains the following things about Hplc chromatography -
1. Hplc chromatography principle
2. Hplc chromatography instrumentation
3. Hplc chromatography types
High-Performance Liquid Chromatography - Other HPLC Types
Ultra High Performance Liquid Chromatography (uHPLC):
Where standard HPLC typically uses column particles with sizes from 3 to 5µm and pressures of around 400 bar, uHPLC use specially designed columns with particles down to 1.7µm in size, at pressures in excess of 1000 bar. The main advantage of an uHPLC is speed. These systems are faster, more sensitive, and rely on smaller volumes of organic solvents than standard HPLC, resulting in the ability to run more samples in less time.
Article source:
hiq.linde-gas.com/en/analytica...
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Thank you for watching HPLC lecture
Got the degree because of you❤😁 thank you sir... from sri lanka
All the best
@@shomusbiologyofficial ❤💪
You explain this better than any of my university professor's ever could. Thank you.
Yeah ..... same here
really
@@faisalali-mz5hs in many universities teachers are lazy
Same
You must be a back bencher lol
omg your the best dude, not even my professor explained it this well. Never stop bro, consider yourself a life saver!
The best explaination on HPLC, ever. I have never heard of any explaination better than yours. I mean my professors dont teach us like that. Thank you, sir.
Glad you liked my lectures. Please subscribe and share
This video is HIGHLY informative and extremely clear. I understand HPLC now, more than I ever have. Wow! Thank you for taking the time to help us all. :)
Better come to our university and teach our staffs how teach the students...Really your teaching is good
Thank you. Glad you liked my lectures
One of the best, if not the best, video for HPLC i've watched. Thank you so much.
I am a MSc microbiology student, I pass my degree bcz of your classes .Now also I used to watch your classes.Thank you very much sir😊
Thank you
Hlo sis I want to do msc biotech which clg is best
I love this tecnology , my final proyect of university was about ultra performance chromatography liquid- spectromatry mass UPLC -QTOF for detected some specific monomers of cutin in fruits , now I follow your job in RUclips I like
Thank you so much for sharing the information! I've been to class and go through the notes given but still I couldn't get in but after I watch this video it really help me to understand the principle of HPLC! Very good explanation and easy to understand!
I've hard problems understanding this topic. Shomu's made it all simple. I will stick by these lectures. Thanks a lot.
Thank you for the all of your explanation videos..most of our university badgemates watch your videos to understand Biochemistry lessons.Thank you From SriLanka❤
Thank you very much, Suman! You explain very well!
Especially the one with the Gas Chromatography lecture. It is very interesting and understandable thanks to you. You know how to teach propely.
No one can't replace u sir...great lecture...keep doing, thank u sir.
Splendid! I didn't need to go through my lecture notes in detail. Thanks
Comrade, your explanations are very simple, well organised and detailed at the same time. You are one of the best teachers on RUclips about chemistry.
Thank you so much for appreciating my efforts
i always go back to yr vid sir since study and now even for my job. thank u so much! best online teacher ever!!
You're welcome. Glad you liked it
Just 5 days to go for our university sem exam..and thanks to god..We have you here with us😍
Appreciate your clarity of thoughts .
Your efforts to promote science thro lectures like this amazes me .
Keep it up and continue your good work .
Thank you so much for appreciating my efforts. Glad to hear that you're getting benefit from my lectures
My favorite teacher, your lesson really helps me a lot. Keep doing so bro. Being a teacher is your precious gift.
Thank you so much for appreciating my efforts
AoA sir i am really impressed by the way u teach chromatography.its remain very helpful for me and clear my doubts, sir thanks alot
Thank you so much, sir! It helped me to have insights about HPLC for my (which is supposed to be our) thesis :)
Thanku sir... thanks a lot for this video, it helps me a lot.....keep going and keep shining🎆🎆
Hi Shomu, i just Watch my first video from you on gas chromatography and it was easy and fun to learn. Thank you.
Thank you so much, Suman & Shomu's Biology, very well explained.
Very nicely and in a very simple language it is explained the complex system.
now i undertand this ! thank you so much
Very nice explained!! I like it so much !! Very nice study I'm proud of sir !!!God bless u !!!
Wow, thank you so much 😭😭you saved my life to think I have an exam in 3 days ❤️❤️ ❤️🙏🙏
All the best for the exam
Very clear about the topic. The best lectures I attend ever 🙏
Glad to hear that you're getting benefit from my lectures
i love the way you explained.. i nver find it so easy.thanks
You can think of the interaction between the sample and the stationary phase as someone stopping to chat with a person they like, which delays them from getting to where they need to be. If you do not like the person, you do not stop to chat and speed past them/avoid eye contact (ei. Hydrophobic molecule zooming by hydrophilic stationary phase.)
Nicely described. Will it be possible to develop and describe a vedio on Glycated haemoglobin ( HbA1c) chromatogram ?
Awesome video!! 😆 VERY INFORMATIVE AND STRAIGHT TO THE POINT.........
Thank you so much 😊 Sir 🙏.
Your video classes are not my first options or last options They are my Only options 🙏
These are Very much helpful.
So many good wishes for u Sir🙏.
Thank you so much for appreciating my efforts. Stay tuned for more
Waoh its straight and the explaination is so clear. thank you
last step of instrument is very helpful to clear confuseness
so again thanks a lot
Your explanation is really nice. Basic concepts was clears specially hydrophobic and hydrophilic. Thank u so much
You're welcome
systematic and simple approach to topics makes it easy to absorb...thanku
Thank you. Glad you liked my lectures
You are explaining complex things in very simple way . I learned a lot thanks a lot..
Thank you. Glad you liked my lectures
You saved me too many times that i can count. Thank you ❤❤
Thank you. Glad you liked my lectures
briefly, nicely explained #HPLC
good speaker thank you sir.....
u make the things very clear and easy. thankuuuuuu so much sir
Tq u Sr u r clases are very help full to undstand than our lecturers....... Tq u so much sr
Very nice presentation .....understand everything in simple path........Thanks alot.....wish you a nice future,,,
You're a legend. Thank you!
Thanking you alot for saving us from boring lectures of our Professors....you are the saviour of millions of the students like me who have not Studied at all during the whole year becoz we knew that we have our beloved teacher Shomu sir on RUclips to teach complicated things just like alphabets. We really appreciate your efforts for making Science easy and lovable.... thnakuhh 😊
@Sagar_University
Thank you so much for appreciating my efforts to help
Thanks!
I really liked the way you explained it. Plain and simple but very informative at same time.
+Dinesh Kumar thank you. Glad you liked it
not all heroes wear capes.. thank you for your efforts
clear d concept of HPLC...thank u Sir. ...good explaination...
Another great video, thank you!
You are really great man thanks for your efforts
Thanks!! What you have mentioned in video is really detailed. And I do need this.
+sabrina run thank you. Glad you liked my lectures
you are eplaining HPLC chromatography much better than my proffessor at university , tanks for that.
You're welcome. Glad to hear that you're getting benefit from my lectures
Good job explaining the basic of HPLC.
You are the best teacher 🧬💕
Thank you so much for appreciating my efforts
Great refresher, thank you!
wonderful explanation brother u done a great video is very useful for pharma students....
Thank you!! Finally I understand that!! 😊
Thank you so much!!! This video made studying this topic so so easy! Thank you so much...
Glad to hear that you are getting benefit from the videos
Good explanation, it is in general, if yiu explain with a protein sample, it is very good to understand what chemicals we can use
Thank you very much .you have explained this technique better than anyone else ☺
Thank you so much for appreciating my efforts
GC is mainly used for volatile and semi-volatile analytes. Biological samples are not ideal for GC-MS not because they are vaporized but they can't be vaporized. Rather, biological samples are thermo-labile and degrade at high temperature in GC column. But, process such as derivatization can enhance volatility and thermo-lability of biological analytes such that you can analyze in GC-MS.
you are really awesome brother i will not able to express my all thank you for you...
Glad to help you out
Thanks you sir for making my problems clear.....
Excellent lecture, appreciate your efforts. Keep up the good work!
Thank you. Glad you liked my lectures
it is always wonderful to learn from you. may Allah always bless you Ameen
You're welcome. Glad to hear that you're getting benefit from my lectures
Nice and clear understanding lecture i have ever seen
this was really helpful thanks so much for the video
but my teacher is making us study a diagram with a pulse damper, drain valve, filter, back pressure and transducer and i've no idea what those are for lol...
I FINALLY GOT IT! YOU ARE A LIFE SAVER!
Thank you
Sir uh olwaz make ma work easier...
Thank uh so very much...
Amazing explanation about HPLC . Very clear concept with basic cover .
Thank you so much for appreciating my efforts
You are explaining a very nice n clear n easy to understand..thank you
You're welcome. Glad to hear that you're getting benefit from my lectures
Amar puro MSc ta tumi bachiye dile. ...thank u a lotttt💐
You're welcome
I wish..you were my teacher in my college...
Now you can learn anytime from my lectures
Dude. Great video. I'm a littlie drunk and i could still follow it. Boom subscribed.
+Manny L that's great. Thank you
i wish we could get you as a professor for our class :)
perfect explanation and easy to understand, keep going... I like this video...
You're welcome
thank you so much... this really help me a lot
THANK YOU for this perfect explanation
You're welcome
Great tutorial. Thanks. Please post a tutorial for how to read chromatogram for quantitative analysis.
I never attend any lecture on HPLC . But iam good at it. 👊👍👍 Waters HPLC+Empower 3 ❣️🔥 good job bro.
Thank you so much for appreciating my efforts. Glad to hear that you're getting benefit from my lectures
I understand HPLC because of you. Thank you.
same with me
thank u thank u soooo mch
u described it so easily.
thank you so much ! this really helped.
+Nour Ghazal glad it helped
Love you Bhai... Another Good lecture... Thank you !
Glad to help you out
Super helpful, thank you very much!!
By the grace of Allah, by seeing ur vdo i got job in reputed pharmaceutical in bd, thanks
Thank you
Figure of merit for analytical method please explain in one video..
you have told the traditional and the reverse phase chromatography as same...the mobile phase as philic and stationary phase as phobic....while explaining... please do check and tell what exactly the difference is
thanq..this video helped me a lot
Very good explanation about hplc thank you sir
Thank you soo much sir ... u can explain very easily .. very helpful to me..
Thanks
very nice presentation, full logic concept on the hplc. thank you
+Yarro Paschal thank you. Glad it helped
Ur explanation was super sir ....thanku...please tell me what is columns how many type of columns
Method of teaching is awesome
Thank you so much for appreciating my efforts
Way of explained..just amazing. nd .understandable,❣️
Thank you so much for appreciating my efforts
This so helpful.
Thank you.
Thanks sir for being the reason to face the exams 🙏
You're welcome
Excellent way of Explanation
thank u for the wonderful explanation
Amazing video great explanation.