Sandwich ELISA assay

[@dharanik.biotechnologyldc9034]

very usefully and now i have got the clear cut idea abtout the sandwich eliza .tx sir u have done a gd job

[@BIBECHYOUTUification]

Shomu's Biology is great source of knowledge. Thank you for this information and keep educating us.

[@shomusbiologyofficial]

Thank you

[@nwilczyn]

all this talk of sandwich ELISA is making me glad it is almost lunch time

[@districthospitaltumakuru1556]

excellent lecture, very simple and easy to understand, thank you so much

[@shomusbiologyofficial]

You're welcome. Glad to hear that you're getting benefit from my lectures

[@dhanrajpaulp6678]

Shomu I love ur way of teaching 💋....

[@shomusbiologyofficial]

Thank you so much for appreciating my efforts

[@isadoras8686]

Your videos help me get through my genetics degree, so straight forward and well explained :)

[@shomusbiologyofficial]

+Isadora Diamond thank you. It's a pleasure to hear that.

[@khurramkhan7313]

i love u

[@sakshichandra7777]

It's very helpful😊..and easy to understand thank you so much..🙏🙏

[@shomusbiologyofficial]

You're welcome. Glad to hear that you're getting benefit from my lectures

[@bbe3331]

Thank you for your wonderful and informative lectures.

[@shomusbiologyofficial]

You're welcome. Glad to hear that you're getting benefit from my lectures

[@sheikhrayees8678]

Thanx sir u make every technique easier to understand keep going and best of luck

[@ErtugrulGhazi-mv8yq]

bht help hori hay bhai shukrya

[@shomusbiologyofficial]

You're welcome

[@urvashibhatara3626]

It was very informative. I have watched your videos on RIA, Competitive ELISA also and liked them. Thank you Sir.

[@shomusbiologyofficial]

+Urvashi Bhatara thank you. Glad you liked my lectures

[@jonathanbensonmugaka7608]

I like the video,it makes me understand all types of ELISA easily

[@shomusbiologyofficial]

You're welcome

[@MdMahmud-vd9ck]

You are great teacher ! Keep doing your good work !

[@learntogether8900]

Thank You Sir. It's very helpful for my exam.

[@shomusbiologyofficial]

You're welcome

[@hasaankhan1048]

Could you talk a little bit about a cell-based assay (CBA)? Much appreciated.

[@ymmalladad2663]

It's Very helpful Sir thank you so much sir ☺☺

[@shomusbiologyofficial]

Glad to hear that you are getting benefit from the videos

[@pieter2160]

Great explanation!

[@shomusbiologyofficial]

Thank you

[@anshumehta8222]

thank you for this explanation
helped a lot

[@shomusbiologyofficial]

You're welcome. Glad to hear that you're getting benefit from my lectures

[@ahmadalsalim9630]

thanks, man very clear explanation

[@twiringiyimanaemmanuel9742]

nice work . Shomu.

[@shomusbiologyofficial]

Thank you

[@meghnajain1576]

You are running an ELISA which uses the polyclonal secondary antibody . What type of ELISA it is? What could be the major issues that can arise due to the use of polyclonal antibody? How will you reduce the background noise in such case?

[@Fry942]

clear explanation

[@shomusbiologyofficial]

Thank you

[@ImranAhmad-df3mi]

Always osm sir nyc explanation as usual thanks a lot Sir

[@shomusbiologyofficial]

Glad to help you out

[@komuravellyvamshikrishna8953]

Tq fir good explaination sir

[@shomusbiologyofficial]

Thank you

[@linadhote7195]

Very good sir...

[@shomusbiologyofficial]

Thank you

[@durgeshsingh5031]

Sir acc. To kuby only enzyme-linked antibody specific for different epitope is added nd thn substrate is added to measure the colour.
But u said we add primary antibody after that another secondary antibody.

[@Parul14315]

Thank you sir 😊

[@shomusbiologyofficial]

You're welcome. Glad to hear that you're getting benefit from my lectures

[@sangeethatalks8250]

Thank you sir♥️

[@shomusbiologyofficial]

You're welcome

[@BinhPhan-wn4dj]

Thank you for your information. Could you talk more about how to the first Ab can capture on assay plate? What is characterization of assay plate for Sandwich ELISA?

[@kashishsahil3546]

really good presentation

[@maryanncrystaluzodinma2391]

good job sir...

[@gayatrideepti6220]

Hello! Very nice lecture 👍. I have a question about ELISA. Is it possible to add the secondary antibody first and the enzyme in the next step in the procedure without combining them both and adding it to the sample . If so, would there be any difference?

[@alrobai]

l looked over all your videos and I didn't find video about pSico nor Lentiviral technologies.
Would you pleeeeeese do a lecture about pSico and using Lentiviral vector technology to deliver the gene of interest?? I couldn't understand these two technology.

[@ankitachoudhury5546]

Is the antibody Z1 and Z2 raised in different species?

[@reshmasamanta4845]

WOW .............NYC SIR.................

[@nehanaranje8134]

thank you
it was very helpful

[@BalaBiologyWorld]

wow fantastic.....

[@messedupkeys8457]

Does the SD HIV1/2 test kit has the same principle as the sandwich EIA?

[@Reni8705]

Thank you so much 🤓🤓🤓

[@kaushiksen281]

It would be really helpful if u can kindly give us an insight about 'Double Antigen Sandwich Elisa'

[@shomusbiologyofficial]

Okay

[@yonasgebrehiwot2260]

GOD BLESS U

[@tamannamalik6931]

Thank you sir.

[@globalbiologicalacademy2784]

Would you please upload a video about Oxidative stress?

[@soumighosh9075]

Why it's more sensitive?

[@akshayavidhya9325]

thank you sir

[@shomusbiologyofficial]

+Akshaya Vidhya you're welcome

[@nawazishaliqazalbash907]

please explain
what is Enzyme-linked fluorescent assay (ELFA)?

[@muthyalakapil8743]

Sir what is the substrate we have add in this reaction.please clarify my doubt sir

[@alkakumari3872]

Thanku sir

[@shomusbiologyofficial]

You're welcome

[@ugurboralfan1]

thank u sir

[@doctoralaaal-wattar2127]

THANKS DEAR

[@umamishra1407]

Sir why we need two antibodies we can also put only one enzym linked antibody plzzz reply.............waiting eagerly for your answer to clear my doubt🙏🙏🙏🙏🙏

[@shomusbiologyofficial]

That can cause u wanted binding and background noise.

[@sadiamumtaz5057]

great sir g, how can v save these videos for offline listening.....

[@paramjitKaur-qx3bi]

Download it.

[@yasmeenzia7844]

ELISA ka lecture kaha hy mujay nhi mil raha ....

[@anilbudumuru3969]

Sir Elisa human body Loki ela enter avutuendi plz reply Sir

[@RitikaVishnoi9]

Why can't we use same antibodiy as the antigen is same??????

[@himansunayak3917]

sir plz can u send me d link of ELISA???

[@ErtugrulGhazi-mv8yq]

sir i want cmplt video

[@shomusbiologyofficial]

U

[@chandanaraghuraj188]

👍

[@debarshinipoddar7493]

Can you please share where after Masters in Medical Physiology can a person apply for job

[@shomusbiologyofficial]

Yes you can apply for job in diagnostics

[@dr.lakshmisruthi3392]

pls improve audio clarity.

[@sudhabalraj9586]

7th day, 10th concept!

[@rahishaaz4392]

Sir...strip based hiv test is which type of ELISA???? Plzzz need answer...

[@debabratadasbarman5283]

Sound is very bad

[@anchlakumari7704]

please send me video from kuby