Radioimmunoassay technique (RIA)

This lecture explains about radioimmunoassay techniques or RIA. Radioimmunoassay (RIA) test is a very important in vitro assay method used to measure concentrations of antigens (for illustration, hormone levels in blood) through use of antibodies. As such, it may be visible because the inverse of a radiobinding assay, which quantifies an antibody via use of corresponding antigens.
Watch this video lecture to understand the mechanism of Radioimmunoassay also known as RIA.
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Thank you for watching the video lecture on RIA.

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awaesome way to express idea about the concept of ria and elisa no other video can substitute this video its a best video of ria

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Thank you sir. I work in a Regulatory Science division. I needed to understand RIAs and now I do.

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[@shrutihsingh]

That was great!! Thank u
But what we studied was there are blocking agent which gets displaced & also the supernatent is discarded rather than detected for radioactivity!! Also conc. is inversely proportional to radioactivity. I think i m more confused now!!

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Thank u for making this technique so easier......concept are well explained

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[@shubhamsonam]

brother can you teach us about reference curve being formed on plotting the concentration of the hot and cold antigens?? looking forward to it.

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[@abdullahmarwat2714]

according to RIA when radiolabeled Ag combine with Ab ,the reaction will occur and it gives a signal like color graph or whatever so, but only free labeled Ag give no result. So concentration of Ag (in patient sample) is inversly propotional to the radioactivity.

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so thankyou.... it is very useful at all...

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[@mimo5463]

Hi Shomu. Thanks for the lecture, very easy to understand and informative. I have a question however. I have watched several other videos on RUclips that say when you have no unlabeled antigen bound to the antibodies, the radiation is at 100%. Here you are saying it's the other way around, that when the labeled antigens are bound to the antibodies it's at 0% (or close to it) and then when the unlabeled antigens compete and bind to the antibodies it is possible to measure radioactivity because of the "free" labeled antigens, meaning the % will increase...
Did I misinterpret something or are there just other ways to measure the radioactivity?

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thank you, so helpful

[@hadisirkhot8907]

How do they displace....if they already attached then how would newly added patient serum ag will compete...

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[@alokdev6671]

It was a wonderful explanation thank you.My question is ,,the more radioactivity means the more antigen present in patient serum and it is directly proportional to the patient Ag serum but in case of hormone is it the same ...? because i read somewhere that hormone in the serum sample inhibits binding of the radiolabbelled hormone thus more radiactivity =less hormone .I would appreciate if you can reply me back to explain it furter .Once again thank you ...you are awesome

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[@kapamed3041]

the video is quite helpful but is it possible to carry out this technique in the reverse manner i.e. looking for antibody when antigen is known? like in the wells will be antigens radioactively labelled and then we introduce patients serum to detect if antibodies to that antigen are present?