ELISA test

ELISA assay - This immunological assay lecture explains about the elisa test procedure and principle behind the elisa assay including direct, indirect and sandwich elisa techniques to determine the presence of antigen and antibody in blood sample or serum sample.
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Thank you for watching the lecture video on Elisa test including direct and indirect Elisa assay.

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[@dawnhoran3353]

As always a perfectly clear and fabulously concise explanation thank you so much. I now always go straight to your lectures for clarity on subjects so thank you very much.

[@imanekabouche3720]

This was very clear, it truly helped me understand this procedure better ! Thanks a lot

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[@sanyarajput6397]

All concepts crystal clear! Thank you 10000000 times. Very helpful ✨

[@shomusbiologyofficial]

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[@cathyyanagi428]

Thank you for making the concept so much easier to understand. You are a great teacher :)

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I can't find this topic in clug and Cummings.your video helped me to know this.topic..thank you sir

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[@Theodore660]

Got my exam in 3 days... thanks for this... saved me a lot of precious time.

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[@jemimahrajakumar7770]

Im kinda disappointed that the pics in the thumbnail weren't used but still this video helped me better than any other, thank you bro

[@jaidevdh01]

Sir i study biotechnology your concepts in videos are very clear and specific thank you 🙂

[@shomusbiologyofficial]

Thank you

[@findyourniche1007]

Finally it clears my concept about ELISA😊

[@shomusbiologyofficial]

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[@shomusbiologyofficial]

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[@abhinavhp9405]

@@shomusbiologyofficial yesss alot.. You're master of all in this.. Best of all😍

[@ahmedeldesouky1878]

your explanation is always great

[@shomusbiologyofficial]

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[@bubbleminho9092]

Really beneficial 🙌🏻🙏🏻like before I didn’t understand anything 😩Thanks a lot 🙏🏻🤝😃😊

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[@shreyasengupta2984]

Awsome...explanation..thank you...being a bsc nursing student...i got my doubts even more clear...thank you vry much sir...

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[@shreyasengupta2984]

Yes sir...thnk u again

[@siphesihlebongiwe1540]

Well explained. Thank you so much, Mr.

[@shomusbiologyofficial]

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[@gnneerajashree5550]

Thank you so much sir.......I watched all your immunology videos and did my exam today very well sir.....Once again Thank you so much sir.....

[@shomusbiologyofficial]

Glad it helped

[@awke5640]

ELISA ASSAY IS A GOOD METHOD FACILITATE TO KNOW OR IDENTIFIED DISEASE EASILY AND THAT DISAESE INCLUDING HORMONES,HBVSAG,RVI ETC.
AND THE LECTURE WAS CUTE AND THE LECTURER WAS DEEPLY EXPLAINS THE ELISA,PROCEDURES,PRINCILES ETC.

[@shomusbiologyofficial]

+Abdisalan Awke thank you

[@Aestheticalien.]

Thankyou so much for your efforts sir😊❤

[@shomusbiologyofficial]

You're welcome

[@mahparakhanam5093]

Thank you for such a good and helpful video...i understood it completely

[@shomusbiologyofficial]

You're welcome

[@gowthamim8986]

Thanks a lot sir!! 🤗
Ur videos helped me at last time of exam..... While I was struggling to understand and I'm scared what to do?? In exam.....
I watched ur video and i did well in exms..... Thank you so much.... Really it's very helpful ✨

[@shomusbiologyofficial]

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[@ifeanyichukwueke9942]

The illustrative teaching helps a lot! Thanks

[@shomusbiologyofficial]

+EKE IFEANYICHUKWU glad you liked it

[@stanzmahiii5819]

God bless uh....I was very unfamiliar with the topic ..u made it superb clear 👌👏🙌🥰🥰🥰

[@shomusbiologyofficial]

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[@renadgalal14]

Thank you very very much
i wish my school teacher explains like you

[@shomusbiologyofficial]

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[@BrownieCraver865]

Thankyou so much! this video is so helpful for me cause I'm studying biotechnology 🙏

[@shomusbiologyofficial]

I am honoured

[@betulceylaner]

This is definitely so helpful, i can say that i understood it finally. Thank you very much

[@harigowthemg]

Dear Mr. Suman,
One thing which wasn't clear to Me is that: Competitive E n Indirect E were alike except for the image of Antigen-Antibody Complex. The result would be the same right for both of them. Can You please explain on how to differentiate among a group of Antibodies??

[@fatinnazurahsaman5692]

+Hari Gowthem Yes I want to know the similar thing too.

[@shahbazalikhawaja1587]

hey
I know its a bit confusing but indirect elisa is for detection of antibodies in serum and competitive is for detection of antigen just like direct but its more sesitive and efficient
hope it helps

[@shahbazalikhawaja1587]

just realised this question was posted a year ago. lol

[@fatinnazurahsaman5692]

Alright no problem. Thanks.

[@bowbowvlog6697]

If u put eng subtitle is more good for hearing impairment ppl

[@nopinkienoluyolo883]

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[@medhykhalil9169]

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[@Neer333]

Wonderful depiction and explanation. Thanks

[@shomusbiologyofficial]

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[@hellobro2465]

One of the best presentation ❤

[@shomusbiologyofficial]

Thank you

[@rachitsrivastava4958]

U are best in this field sir

[@shomusbiologyofficial]

Thank you

[@naureensharmi9262]

Your videos are so perfect! 😍

[@shomusbiologyofficial]

Thank you. Glad you liked my lectures

[@Deepikaaryarsp]

Thank You so much sir... your lectures helps me a lot... 😃

[@shomusbiologyofficial]

Glad you liked my lectures

[@vishalbandi9188]

Thanks sir it claarified my concept

[@shomusbiologyofficial]

Thank you

[@maheendhanani4212]

thank you!
i have one question though, how do you know which antibody to add in the beginning though?

[@akashvani3836]

Actually ELISA is done for a specific test like AIDS DENGIE etc... so the antibody differs for different tests...for further queries you can ask...

[@kaneezfatima5144]

The specific antibody atificially prepared used to be there in labs.
Say if we(doctor) ask a patient to get a HIV test his serum is put in that well which would be already having HIV antibody so if he is HIV positive his serum would be surely having antigen that would bind the antibody of that well....

[@Sheezzz123]

You are such a heaven sent. Thank you for explaining things so well!

[@shomusbiologyofficial]

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[@nimrailyas7120]

Thankyou sir ✨ understood 🤟

[@shomusbiologyofficial]

You're welcome

[@hangoo91]

Very good explanation thanks a lot

[@shomusbiologyofficial]

You're welcome

[@aristeiasales7194]

Thank you. Very clear explanation :)

[@aishaanwar6287]

thank you so much for explaining so clear

[@shomusbiologyofficial]

You're welcome

[@Ramachandran-io2eo]

Thanks sir.
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[@daniel_joseph_73]

Thank you very much..

[@shomusbiologyofficial]

You're welcome

[@uma8452]

Thanks a lot! I couldn't get a clear fundamental understanding from any online source or journal so far. This has been very helpful.

[@shomusbiologyofficial]

All the best

[@JackSpear1000]

Was Direct Elisa the very first type of ELISA?
Basically identical to the overview technique.

[@98KINJAL]

No I think that was the Sandwich Technique ...not direct ELISA

[@DrAmiry]

Your videos are helpful, however, in this particular video I could not figure out the difference between indirect and competitive ELISA. In both, we add blood serum and the antigen and antibody interaction occurs. Therefore I think it needs more elaboration. Anyway, Keep your good work up!

[@habeebhussain2029]

That was amazing sir...my doubts were clear up..thank u soo much....

[@shomusbiologyofficial]

+Habeeb Hussain thank you. Glad you liked my lectures

[@sameeraboh2050]

How good explanation!! Thanks

[@shomusbiologyofficial]

Glad to hear that you're getting benefit from my lectures

[@gohilhiten6377]

Can you explain me why there is use of 2 type antibodies (antigen-antibody complex and only antibodies) in competitive elisa method??

[@ahmedeldesouky1878]

thank youuu so much

[@shomusbiologyofficial]

You're welcome

[@charleszhou5021]

Hi, we are producer of guanidine thiocyanate which is a important raw material for ELISA.hope we can discuss further. thanks!

[@manasiovhal3134]

Thank you 👍

[@shomusbiologyofficial]

Thank you

[@Slowworld2.o]

very helpful for me as one day ago of my exam................thnkyu

[@Ramya.85]

nice explanation thank u

[@snehaar1814]

thank you these are very helpful for all my semisters

[@Stop-and-listen]

So the Fc construct of the antibody is attached to the surface of the well? How do they do that?

[@funnytakarar4105]

Thank you sir ... lectures helps me alot

[@shomusbiologyofficial]

Thank you. Glad you liked my lectures

[@ajitdardare4565]

You are superrb bro.....this is fantastic video

[@shomusbiologyofficial]

Thank you. Glad you liked my lectures

[@shanzajamil8516]

once again You saved me thanku so much for the gret videos and superb explanation :)

[@supravomandal515]

Awesome sir!! Thank u so much

[@shomusbiologyofficial]

You're welcome

[@shwetadubey1546]

Thanku very much 🙏👍

[@shomusbiologyofficial]

You're welcome

[@jeel7246]

Competitive ELISA is understood by many of students!!

[@dedicatedtorbc2588]

Thank you sir

[@shomusbiologyofficial]

You're welcome

[@gurleensaini9056]

Very good sir 👌

[@shomusbiologyofficial]

Thank you

[@aakashmore9822]

Thanks 😊

[@shomusbiologyofficial]

You're welcome

[@jaliamutumba2584]

thank you man , your the bestt

[@shomusbiologyofficial]

Thank you

[@artisthindustani]

Please make video on cylinder elisa

[@Zumba_with_adarsha]

Well explained

[@shomusbiologyofficial]

Thank you

[@patriciaisaac4189]

Lovely and clear

[@shomusbiologyofficial]

You're welcome

[@ayeshakhatoon6470]

Where is incubation or washing step with buffer in ELisa method ????

[@pushparajsingh2553]

Good afternoon sir
Thanking you sir

[@manubashir554]

Very helpful 😊

[@shomusbiologyofficial]

Thank you

[@muzzammilabdullahiyunusa4509]

Very educative tutorial bro.

[@jinatamanhussain4811]

Thanks

[@shomusbiologyofficial]

You're welcome

[@kathyc3005]

Thank you so much for the very informative explanation. I'd like to add that in the sandwich ELISA the first and second antibodies are called captured ab. and detection ab., respectively.

[@Bhaventh]

❤️ from an Engineer

[@shomusbiologyofficial]

Thank you

[@priyankatiwari3434]

thanks again..

[@ouafaoumaima4628]

thank you 😊😊 you are a life saver 😆

[@shomusbiologyofficial]

Thank you.

[@physioconvidyasagarunivers5368]

you doing a great work.carry on god bless you

[@shomusbiologyofficial]

+Kiranmay Chauliya thank you. Glad you liked my lectures

[@akashvani3836]

Sir please explain in case of indirect ELISA how 1st antibody can attach to 2nd antibody (as we know antibody attached only with antigen)

[@swisstravelers1115]

Secondary antibodies are anti-species antibodies, therefore those can bind the primary hoste species antibody.

[@vishalmayur141]

Tq sir for this video

[@shomusbiologyofficial]

You're welcome

[@akashkharwar8821]

why in competitive Elisa we not call secondary antibody to a enzyme linked antibody ?

[@mumnetamuk419]

Ur my saviour 😎

[@shomusbiologyofficial]

You're welcome

[@usrarahim9369]

Thnx alot👍 that was of great help indeed...

[@gayathriarun2530]

Why don't enzyme react with substrate before attaching to the antigen ?

[@DomiojyiX]

I just searched my name 💀

[@smahi4479]

This video is good as always....But can't download the ppt for this topic.

[@shomusbiologyofficial]

You're welcome

[@meenakshibisht3556]

Thanks a lot for this

[@shomusbiologyofficial]

You're welcome

[@meenakshibisht3556]

Can you tell me in rnai jb sirna risc se judta h tou phir sirna ke sath kya hota mtlb wo D's sirna as sirna me belts ????

[@meenakshibisht3556]

I watched your video but mje Kuch samj nhi aya

[@charleszhou5021]

Hi,this is Charles Zhou from SMART CHEMIALS, We are producer of guanidine thiocyanate.glad to you are a export of ELISA kit. you may be intersted in guanidine thiocyanate.hope we can discuss further. thanks!

[@drabinash1998]

Excellent

[@shomusbiologyofficial]

You're welcome

[@muskanmunjal2949]

why cant the floating antigen antibody complex attach to the enzyme bound antibodies? do the antigen antibody complex and the free antibody of different nature in competitve elisa?

[@collegediaries4259]

Thank you sir 😊

[@shomusbiologyofficial]

You're welcome

[@angeloorsini1764]

U missed the Direct Elisa? or am i wrong?

[@josiachendi]

he mentioned it, for the direct ELISA the first antibody is linked with an enzyme, the need for a second antibody is no longer needed

[@sidratulmuntaha1687]

yes he didn't give the full explanation of it...like how primary Ab is enzyme linked...obviously it might not be from the serum...then it gets coated to the Ag being attached to the well...i think the Ag is taken from the serum and coated to the well but how??? or may be the Ab are taken from the serum n attached to the enzyme...like how?? it need to be explained

[@Kwasi-Quan]

Very helpful Thank You 🙏🏾

[@prasanrajj]

u r an inspiration

[@ankitray9250]

Thanks sir, it was amazing. Cleared a lifelong doubt

[@SuperShreya999]

Thanks very helpful

[@shomusbiologyofficial]

You're welcome

[@tejasdev5774]

sir why you did not cover other types of Elisa like cassette Elisa. it would be a great help if you do so.