I am a biology graduate and working as an laboratory analyst for 1.5 years. For I only working with traditional microbiology testing procedures, it is so hard for me to understand how PCR works. This video helps me a lot as a total beginner in molecular biology. Thank you USDA and Dr. Alexandra.
Great in depth video about qPCR set up and data interpretation. Extremely useful for beginners and a great refresher for more experienced users. Lots of great practical tips and theoretical considerations. Dr. Scupham, you rock!
The lady in this video did a better explanation of PCR than my professor did both in theory and in lab! Thank you so much! It all makes sense to me now!
Thank you very much for sharing your insights and knowledge filled videos !! Professional and informative !! Outstanding !! Greetings from California … I wish you and folks good health , success and happiness !! Much Love ✌️😎💕
Awesome video on qRT-PCR. Learnt alot for personal development. Thank you Dr Lexa Scupham for this educational video as a lab personnel. Thumbs to you and your team.
Thank you so much, Dr. Lexa Scupham, for this amazing video. It is clear and very informative. I took some notes to help me with my experiments and protocols. I can say that now I understand how real-time PCR is performed.
you did a great effort Dr. I must say that it is one of the best tutorial with practical information and details on real-time PCR. thankyou so much. you rocked. Dr. Sumaira
Wow, this is a great video. I’m not only talking about qPCR but in general lab work. Also amazing and clear explanations and suggestions. It was really interesting and I actually enjoyed it a lot. Thank you so much!!!
sometimes when i take like 0.5ul dna or 12ul mix there will be always leftover liquid in the tip that doesnt want to come out. is it because my micropipette is not good anymore or it just the way i use it is not proper?
Do you have any videos on how to optimize PCR conditions for a new set of primers, starting with temperature? Is it common to run a temperature gradient in qPCR?
I'm facing a major issue with contamination during qPCR at work. For the last 6 runs, my NTC has been showing a strong curve, which shouldn’t be happening. I’ve tried various troubleshooting steps, including purchasing three different sets of new probes and primers, and even quantified the water used as NTC (with a Qubit reading of
I liked the way you were working and the degree of organization you have . I would love to have a chance to be able to work with you, as i think you would be a great mentor for me . thanks in advance.
Dear Madam, pls accept my gratitudes. you are an an gel for me.I am struggling with qPCR and did not know how to start! can you please tell me the paper work you did for your lab pcr, how can I set mine for my lab?
Hi, madam I watched this video and really understand your explanation. Please do a favor, I need video like this for agrobacterium infiltration in tobacco or etc. Thank you
Great Job!, thanks. I wonder where to get/prepare the starting materials for the standard curve. How to know the number of copies of my plasmid in the sample. Is this a linear DNA plasmid or RNA transcript?
can anyone help mee pleasee... i want to separate mRNA(bax gene) from rat brain cells...and later wana do rt pcr to check bax expression level.. how will i know that the RNA which i separated is BAX...because there are many other mRNAs present in that cell.. please clear my confusion
This video is so informative; I have learned a lot. Thank you for putting this together ❤ In the preparation of the standard, did you add 5 micro liters from the 10:1 tube to the 10:0 tube? I am a bit lost there.
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Actually I have never found such an expertised content anywhere! I really do appreciate the quality of both information and quality of images!
Not to be dramatic but this video changed my life. Thank you so much.
how? genuinely asking
I have never seen such an informative and comprehensive video on qRT-PCR. Thank you so much, ma'am.
18:20
I am a biology graduate and working as an laboratory analyst for 1.5 years. For I only working with traditional microbiology testing procedures, it is so hard for me to understand how PCR works. This video helps me a lot as a total beginner in molecular biology. Thank you USDA and Dr. Alexandra.
I Am A LAB Techinital and I werked For 3 yers and am learnig adegree program so thank you for this pcr system used you continu I like you Dr Alexandra
Great in depth video about qPCR set up and data interpretation. Extremely useful for beginners and a great refresher for more experienced users. Lots of great practical tips and theoretical considerations. Dr. Scupham, you rock!
The most comprehensive video on RT-PCR I have ever seen. Thank you very much Dr. Lexa Scupham 🙏
The lady in this video did a better explanation of PCR than my professor did both in theory and in lab! Thank you so much! It all makes sense to me now!
this is by far the best PCR demonstration I've seen. Thank you!
THE BEST TUTORIAL I HAVE EVER WATCHED. YOU ARE GREAT
Thank you very much for sharing your insights and knowledge filled videos !! Professional and informative !! Outstanding !!
Greetings from California … I wish you and folks good health , success and happiness !! Much Love ✌️😎💕
One of the best videos I have seen to date!
Thank you very much for this detailed and comprehensive video, Ma'am. Please keep creating and sharing more such videos.
very good and thorough video. Better than some undergrad labs. Thank you
Awesome video on qRT-PCR. Learnt alot for personal development. Thank you Dr Lexa Scupham for this educational video as a lab personnel. Thumbs to you and your team.
you really nailed it amazing each and everything very well explained it helped me in my research a lot
Thank you so much, Dr. Lexa Scupham, for this amazing video. It is clear and very informative. I took some notes to help me with my experiments and protocols. I can say that now I understand how real-time PCR is performed.
Very detail and informative , thanks a lot.
well done, it was fantastic, I love the way you mentioned the details and cautions, truly thanks for that
you did a great effort Dr. I must say that it is one of the best tutorial with practical information and details on real-time PCR. thankyou so much. you rocked. Dr. Sumaira
Wow, this is a great video. I’m not only talking about qPCR but in general lab work. Also amazing and clear explanations and suggestions. It was really interesting and I actually enjoyed it a lot. Thank you so much!!!
This is the best class ever. very informative
Informative, to the point and comprehensive, Thanks a lot
OMG .... what an excellent video
Great and in depth video about qPCR. It is really helpful and informative to beginners like me. Thank you Dr. Scupham.
Wonderful thanks for your great work
Thank you so much...learnt a lot. Now i can level up my efficiency.
Expertise matters ... Lots of respect and love. Great demonstration 🪧
Detailed step by step guide on qPCR analysis, great video, thank you so much for sharing!
Thank you Prof!!
Great demonstration to teach newcomers to PCR protocol
❤amazing .Tqsm for sharing your experience and knowledge
WONDERFUL PRESENTATION
Thankyou .Madam..it was a great real time experience with your beautifully explaination in simple way..
Thank you Dr. Scupham for this wonderful video.
Thank you very much for this great video. Love from Tunisia 🙏
This video is very informative. Thank you so much for Explaining the PCR. I had no idea what PCR was! Your video is very helpful!!!
I wish I could like this video a million times,Dr you did a massive job.Thank you😍
This video is so informative; I have learned a lot. Thank you for putting this together ❤
Best video on RUclips.
very wonderful teacher, so fulfil and hope for more of this
sometimes when i take like 0.5ul dna or 12ul mix there will be always leftover liquid in the tip that doesnt want to come out. is it because my micropipette is not good anymore or it just the way i use it is not proper?
are there other videos like this on other experimental methods in molecular biology?
Really Appreciating
Thank you for the video. But why didn`t you use gloves at the beginning?
thank you Dr. Lexa the demonstration is simple for beginners like me, I quite enjoy the video
The lab safety/good manners tips were terrific!
It's been so informative and thanks for doing this video Dr.
Do you have any videos on how to optimize PCR conditions for a new set of primers, starting with temperature? Is it common to run a temperature gradient in qPCR?
Great effort dr. Lexa, highly appreciated your efforts in this regard.
I'm facing a major issue with contamination during qPCR at work. For the last 6 runs, my NTC has been showing a strong curve, which shouldn’t be happening. I’ve tried various troubleshooting steps, including purchasing three different sets of new probes and primers, and even quantified the water used as NTC (with a Qubit reading of
Great simple and straight to the point!
This is a great full overview of qPCR. Thank you, Lexa.
Nice video but can't you use the autoclave machine to sterilize the tools
A High quality video
extremely important video and an excellent teacher. Thank you
Most informative vedio madam
I liked the way you were working and the degree of organization you have . I would love to have a chance to be able to work with you, as i think you would be a great mentor for me . thanks in advance.
It was really greate.. But can u provide the softcopy of the information sheet you used in the video.
This is a really informative video! Well done.... Thank you very much!
Hello, could you please give us the information in the technical shit the paper contening all information about hte volume of each component thanks
excellent thank you.
Dear Madam, pls accept my gratitudes. you are an an gel for me.I am struggling with qPCR and did not know how to start! can you please tell me the paper work you did for your lab pcr, how can I set mine for my lab?
Hi Dr. Lexa, can I get a copy of the Paperwork template?
Thank you so much madam for this detailed and in depth video.
pls send some some sheet if possible because sheet shown in this video are not properly visible.
thanking you.
Thanks for this wonderful tutorial!
nice madam .. very informative
Can someone explain the dilution series she did I am lost by the math on those for this video.
thank you for this comprehensive video 🤍
Is there a similar video preforming a gc-ms ?
it was greattt thank youuuu
Do you have any spots for hiring?
The master mix template is not clear
Good job Hannah and Dr. Lexa!
Thank you. You are life saver. 🙏
Dude this is such a good video holy shit
Wow. Amazing
Thank you so much. Indeed, very informative.
Indeed helpful
Hi, madam I watched this video and really understand your explanation. Please do a favor, I need video like this for agrobacterium infiltration in tobacco or etc. Thank you
Is it absolutely necessary to linearise plasmids before RTPCR?
Yes , I think so.
wow i love this contenent. !!!
why no gloves?
She did not glove up during instrumentation however she did during the process
Thanks very good explanation along with the work
Well done description fully understood
Thanks for your sharing.It's very helpful
What is the differences between qPCR and RT PCR?
Thank you Dr. Lexa. I enjoyed your video, its clear and informative . How about nested PCR?
Great Job!, thanks. I wonder where to get/prepare the starting materials for the standard curve. How to know the number of copies of my plasmid in the sample. Is this a linear DNA plasmid or RNA transcript?
Great. Learned a lot.
can anyone help mee pleasee...
i want to separate mRNA(bax gene) from rat brain cells...and later wana do rt pcr to check bax expression level..
how will i know that the RNA which i separated is BAX...because there are many other mRNAs present in that cell..
please clear my confusion
Great video!😆
Dear madam nice over view on Real time PCR. The primers used in real time PCR can be used in normal PCR without probe
This was helpful and very informative. thumb's up
Really Easy and very Realistic.
Many thanks to you, doctor.
I wonder if you generously explain how I can get the " working paper" 👍🌹
This video is so informative; I have learned a lot. Thank you for putting this together ❤
In the preparation of the standard, did you add 5 micro liters from the 10:1 tube to the 10:0 tube? I am a bit lost there.
best video ever seen
Thank you sooo much mam for beautiful detailed explanation.😃😇😊