Gel filtration chromatography in 5 minutes | Size Exclusion Chromatography | Lab Procedure

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  • Опубликовано: 25 ноя 2024

Комментарии • 64

  • @nikolaspais9029
    @nikolaspais9029 4 года назад +24

    thank you so much i study biochemistry and this video was really helpful. keep up the good work!

    • @animatedbiologywitharpan
      @animatedbiologywitharpan  4 года назад

      Please share my channel link with your friends and help me to reach big audience

    • @maham671
      @maham671 Год назад

      Me also studying biochemistry

  • @NEBULAFITNESS9885
    @NEBULAFITNESS9885 Год назад +7

    This video was was helpful for me in microbiology , thanks sir 🙂

    • @animatedbiologywitharpan
      @animatedbiologywitharpan  Год назад

      Please subscribe to my second channel where I upload career advise youtube.com/@letscrackbioexams94?si=Hhovo7iVObYqyZ5l

  • @PriyankaPrasad0811
    @PriyankaPrasad0811 9 месяцев назад +1

    Bro thank you for this, like genuinely. I'm currently cramming for my first biochem exam and you did in five minutes what my professor couldn't do in two weeks :P

    • @animatedbiologywitharpan
      @animatedbiologywitharpan  9 месяцев назад

      Could you please help me by sharing my contents with your friends group/ college group. I put huge efforts in making these videos but unfortunately not a lot of people are watching this.

  • @shahabkhan7604
    @shahabkhan7604 10 месяцев назад +1

    Would be nice if you add lil bit description, written form as summary, one liner so we may revise things from that

  • @abhiruchisah1595
    @abhiruchisah1595 2 года назад +1

    thank you .You explaine it very nicely .Im from iiser tirupati pursuing majors in biology

    • @animatedbiologywitharpan
      @animatedbiologywitharpan  2 года назад

      Really glad to know it was useful. Please follow my instagram page and facebook page. Please share my youtube channel link with your friends and help me to reach big audiance
      I'm on facebook & Instagram as @animatedbiologywitharpan. Install the app to download notes and flash cards. instagram.com/invites/contact/?i=1p41h314q3fv8&
      You can support the channel by clicking on the super like icon below the video ( a heart sign with $ in it ) . You can support using paytm/ phone pe/ gPay / paypal. Your small contribution means a lot for me

  • @generendellaquino5944
    @generendellaquino5944 3 года назад +7

    Thank you so much for this! Keep up the good work :)

  • @KCGLOBAL2024
    @KCGLOBAL2024 3 месяца назад

    I love this
    Please don't stop now that I'm in school

  • @SulekhaJharimune
    @SulekhaJharimune 3 месяца назад +1

    Amazing work!! Keep it up 👍

    • @animatedbiologywitharpan
      @animatedbiologywitharpan  3 месяца назад +1

      Could you please help me by sharing my contents with your friends group/ college group. I put huge efforts in making these videos but unfortunately not a lot of people are watching this.

  • @laibanadeem2664
    @laibanadeem2664 6 месяцев назад +1

    You explained really well....

    • @animatedbiologywitharpan
      @animatedbiologywitharpan  6 месяцев назад

      Could you please help me by sharing my contents with your friends group/ college group. I put huge efforts in making these videos but unfortunately not a lot of people are watching this.

    • @laibanadeem2664
      @laibanadeem2664 6 месяцев назад

      @@animatedbiologywitharpan ok

  • @avneetkalra1145
    @avneetkalra1145 3 года назад +2

    thank u for making it alot easier

    • @animatedbiologywitharpan
      @animatedbiologywitharpan  3 года назад

      Please share my channel link with your friends and help me to reach big audience

  • @prakashvincent9836
    @prakashvincent9836 4 года назад +5

    Hi your explanation is good. Just a concern is, in the 0.33 time, you tell that there is binding of proteins in gel filtration column which is wrong

    • @animatedbiologywitharpan
      @animatedbiologywitharpan  4 года назад

      I think you got me wrong @ .33 sec I was talking about a general workflow of column chromatography and later pointed out that GFC is bit different than the general workflow ,it involves seiving mechanism in contrast to binding

  • @KokoriKid1
    @KokoriKid1 Год назад +1

    Great video man

  • @udayselva1476
    @udayselva1476 Год назад +2

    Good job brother 💓👌👍

    • @animatedbiologywitharpan
      @animatedbiologywitharpan  Год назад

      Please support my channel via super thanks. Your small contribution like 1$ means a lot for us. The super thanks option is present at the lower right corner of any video, a heart sign with $ in it.

  • @patriciajimenez492
    @patriciajimenez492 3 года назад +2

    can the elution volume be greater than the total volume of the column?

  • @kimpereira4770
    @kimpereira4770 3 года назад +2

    This helped so much! Thank you!

    • @animatedbiologywitharpan
      @animatedbiologywitharpan  3 года назад

      Please share my channel link with your friends and help me to reach big audience

  • @laiqaaftab8555
    @laiqaaftab8555 Год назад +1

    what is wash in workflow

  • @sammrayounas2681
    @sammrayounas2681 4 года назад +1

    Suppose if exclusion range is 5 to 600 then the molecules whose size is below 5 they also trapped between the voids then these molecules also eluted with small size molecules then our reqired size protein will not be obtained is this happen or not sir?????
    Kindly tell me...

  • @palvoliuto6059
    @palvoliuto6059 3 года назад +2

    thank you for your great videos. could you please answer a question for me. what type of chromatography would you recommend for filtration of a polypeptide fragment? Thank you.

    • @animatedbiologywitharpan
      @animatedbiologywitharpan  3 года назад +2

      If the peptide fragment is previously tagged by his tag / HA tag then one can do affinity chromatography, if slight estimation of size is known then one can do gel filtration, if amino acid composition is known and it has charged residues then one can go for ion exchange as well. So it really depends on what we know about the polypeptide.

    • @animatedbiologywitharpan
      @animatedbiologywitharpan  3 года назад +1

      Please share my channel link with your friends and help me reach out to big audience

    • @palvoliuto6059
      @palvoliuto6059 3 года назад

      @@animatedbiologywitharpan Thanks you so much my friend! All the best!!!

    • @palvoliuto6059
      @palvoliuto6059 3 года назад

      @@animatedbiologywitharpan i will and i have subscribed too!!

  • @debarghyapratimgupta9487
    @debarghyapratimgupta9487 4 года назад +1

    Great.. Thank you!

  • @syedtoufeeqahmad9023
    @syedtoufeeqahmad9023 3 года назад +2

    Thank u sir

  • @hccc8782
    @hccc8782 7 месяцев назад +1

    very useful

    • @animatedbiologywitharpan
      @animatedbiologywitharpan  7 месяцев назад +1

      Could you please help me by sharing my contents with your friends group/ college group. I put huge efforts in making these videos but unfortunately not a lot of people are watching this.

  • @Suraj_gawali
    @Suraj_gawali 2 года назад +1

    I watching your playlist

    • @animatedbiologywitharpan
      @animatedbiologywitharpan  2 года назад

      Please support my chabnel such that i can bring more cobtents like this. You can support the channel by clicking on the super like icon below the video ( a heart sign with $ in it ) . You can support using paytm/ phone pe/ gPay / paypal. Your small contribution ( starts from 40rs) means a lot for me.

    • @Suraj_gawali
      @Suraj_gawali 2 года назад +1

      What is your education.and what perpose of making vidio

    • @Suraj_gawali
      @Suraj_gawali 2 года назад +1

      @@animatedbiologywitharpan bro where are you form ? Give me your Mobile no.

    • @animatedbiologywitharpan
      @animatedbiologywitharpan  2 года назад

      Go to the about section in the channel you would get everything.

  • @khadojaraad
    @khadojaraad 2 года назад +2

    Thank you ,can you put translating in to Arabic?

  • @ericajanebone9609
    @ericajanebone9609 Месяц назад +1

    Whats elute

  • @awantikatiwari8080
    @awantikatiwari8080 4 года назад +1

    What does that G represents in that beads chart

  • @vietthangduong7629
    @vietthangduong7629 3 года назад +1

    god blesses me pass my text. make my teacher see this cmt plz T.T

  • @PrinessSonali
    @PrinessSonali 4 года назад

    Sir how to score well in sec C?

  • @ethanferrer1577
    @ethanferrer1577 3 года назад +2

    Redemption

    • @nerocean09
      @nerocean09 3 года назад

      yoooo my slime, i know you dont know mandem like that

  • @prasadpitagi8787
    @prasadpitagi8787 3 года назад +1

    The explanation and animation are great, but your accent in irritative. At some point of time in this short video it drew my attention away. Speak either in Indian accent or western.

    • @babangapu8189
      @babangapu8189 3 года назад

      So is your accent british? Or like your idol sachin tendulkar

  • @harmohitkaur9867
    @harmohitkaur9867 4 года назад +2

    yo can you not use the r slur thank you. if you don’t know why it’s offensive even in a scientific context, look it up.