Salting in and salting out | How does salting out happen? | what is salting in of proteins?
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- Опубликовано: 5 окт 2024
- This video explains the concept behind salting in and salting out in details but within 5 minutes. this video will answer following questions
What is meant by salting out?
How does salting out happen?
What is salting out in protein separation?
This is the best video for explaining the concept of Salting in and Salting out.... Thankyou dada for this....l would like to ask you about the reference where l can get the exact text of this explanation!!!!!
There is no 1 proper source for it ....several review articles and text books . But you can follow voet and voet
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@@animatedbiologywitharpan Ok dada....l will try to look into them
@@animatedbiologywitharpan Sure
Is there any video of Parkinson's disease made by you?
Thank you. You did something my textbook and my lecturer couldn't do.
no is because is very boring why do I have to study stupid biochemistry for my degre what a joke bro lol
our teacher's explaining his subject with ur video, you can be proud bro
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@@animatedbiologywitharpan She shared it with me!!! I've learnt a lot!
I usually have a hard time understanding how some of these processes work but you did an amazing job at explaining the process! I feel like I understand it and could explain how it works to another individual now. Thank you!
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Such a great refresher a year after my biochemistry courses! The graphics are really helpful, great work!
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You are a ray of hope for all of us aspirants like me 🇮🇳🇮🇳
You are legit making biochemistry easy for me 🙌
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Short and clear, thank you!
I was struggling with this concept but you sir explained it very well. Thank you 🙏🏻
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Great work, thank you for your presentation, it was clear and informative. the use of graphics greatly helps, keep it up! Would love more about protein separation and electric properties.
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Thank you for this lecture video. I have never understood this topic before and the mechanism behind it. This video has done it for me.
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finally it's crystal clear to me...thank you so much!
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BLESS U BRO ,SERIOUSLY 🥺😭u saved my ass in 12 hours i have my exam and this made me finally understand
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I love how you teach it. Even a dummy like me can go through medical school just by watching your tutorials.
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Thank you!!! This video was very well explained and helped me understand something even my professor could not help with
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Great video! Just have a litte remark:
At 1:52 you mark the carboxylate ion which is part of the protein peptide backbone. Instead you should have marked the carboxylate at the amino acid side chain which is exposed to the enviroment.
Thanks for your suggestion 😊
Best video ever and very easy to understand! i literally understood everything in 6 mins thank you so much! you are an amazing teacher!!
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Helps a lot! Thanks!
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You saved my day within 6 mins🤗 Thank you!
Utilize the rest of the day by doing something creative 🙌
Best explanation for salting in and out. I want to cite this reference
Haha. Thank you so much
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@animatedbiologywitharpan
I am really interested in your video!
Could you let me know more about the detailed mechanism of the Salting-in step? From your video, I understand that adding salt ions can make the structure of the protein stable along with reducing of the intermolecular replusion of the protein. Then, what is the mechanism of the water molecular can form the hydrate shell around by the protein?
Is hydrogen bonding on the hydrophilic regions of the protein?
Thank you!!!!
Thanks you so much dear , I have finally clear my concept with this video . Thanks to you once again .
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@@animatedbiologywitharpan sir please make a video on water and their bond forming mechanism with biomolecules . Please sir this mine humble request to you.
Awesome explanation. You did a finer job at explaining this concept than my professor. Thanks again.
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Ammonium sulphate precipitation follows the procedure explained by you. It is the good and simple method instead of ultracentrifugation and other methods. Nicely explained. Continue.
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Please requesting you send the procedure to me
Thank you so much for making this fantastic video ❤
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Our group just used your video and it was very informative about explaining the topic. Thank you!
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Thank you for this. Was able to understand it better thanks to this video.
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Thank you so much for the explanation 🙏. Your wise way to explain alongside the illustration helped me in clarifying some points about the topic.
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Less time with clear concept great 😊 thanks for this 👍
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I can understood so easily, Very good explanation sir
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1 hour of lecture in 6 minutes... thanks!! :)
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Thanks alot for the video my concepts are Cleared very well.
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Tysm, this was really useful and informative for me. I’ve just had a lecture about protein separation at the university and got confused but your animation helped me so much
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what a legend. explained better than my uni
Share in your college group
Very informative. Quick and easy to understand. Animation make it more clear.
Really glad to know it was useful. Please follow my instagram page and facebook page. Please share my youtube channel link with your friends and help me to reach big audiance
I'm on facebook & Instagram as @animatedbiologywitharpan. Install the app to download notes and flash cards. instagram.com/invites/contact/?i=1p41h314q3fv8&
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You make it easier than I thought, thanks a lot dude.
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@@animatedbiologywitharpan one of my friends share it with us in the group 😭❤️
Ahmad Abdullrahman check out all of my playlists....might be useful
Really useful video. Such a tricky concept made like easiest thank you 👌🏻
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FINALLY, I understood this. Thanks so much!
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it was an awesome video. you explained so well. thank you soooooo much
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Thank you for this video it was explained very well. Got in on the first take!
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Jazak Allah khyra sir
Thank you for explaining this concept clearly!!!
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What a great video. Thank you very much
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Very good explanation.Thank you very much!!!!!
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Thank you - very clear and concise explanation!!
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Thank you for your such nice and clear cartoon!
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very nice and simple, thank you
Could you please help me by sharing my contents with your friends group/ college group. I put huge efforts in making these videos but unfortunately not a lot of people are watching this.
I like your animations it helps give a clearer pictuce
It was very helpful and easy. Thanks
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Thanks ! I was looking for salting out mechanism in saponification. I hope the mechanism will be similar, as both protein and soap are polar. Thank you !!
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full of knowledge
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Concept clearly explained❤
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Wonderful 💯💯
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Explained in easy way, with drawings - the best videos 😍 thank you for your work
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This video was very helpful! Thank you! Keep up the excellent work!
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Clear and amazing explanation. Keep up the good work!!
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Thank you! This helped a lot! I really appreciate your videos.
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Thank you ! The concept is clear for me now😊
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The video is useful, thank you
it was very informative and very clear. Thank you so much
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Thank you so much! Your explanation was very clear!
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This makes so much sense! Thank you.
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Thank you YOU'RE THE BEST👍
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Thanks! It was clear and easy to follow.
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nice explanation, thank you for explanation its very informative and helpful
however i have a question, regarding the protein isolation, if i not sure about the solubility strength of my desire protein, then salting in (addding low concentration of salt solution to allow it to solube is better) or salting out is better. And base on explanation, i understand that both salting in and out is helping to isolate, is there any condition like protein that not likely to soluble will be favor for salting out process? if there is, is the example fibrous protein will be more easier to seperate in salting out or salting process? Thank you for answering, look foward to knowing more on this
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Excellent video..m
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best explanation ever!
ruclips.net/channel/UC4IpyopsGWSjaPACNTZLuqg please subscribe to my second channel as well
Beautifully explained thank you! I've been struggling with sample preparation for months now - I have VERY expensive triple-labelled samples for NMR so, naturally, sample stability has been a great concern to ensure we can get the most out of each prep.
Have you got any rough kind of buffer optimisation "workflow" that you go through when determining protein solubility? I've thus far tried crude hanging drop precipitate screens using a crystallographers microscope, as well as a thermal denaturation assay utilising a hydrophobic residue-binding dye which infers protein unfolding using qPCR (ThermoFluor I believe is the technique name).
Have you got any other suggestions? I'm still struggling somewhat with long term stability.
Literally bro.. Yor presentation is too good!! Thanku 👌👌👌
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Thank you,You understood me very well،Keep making more videos🌹🤍
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I appreciate your work sir thanks 😊
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very nice and point wise explination.Thank you a lot.
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Great Animations
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great and clear explanation
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@@animatedbiologywitharpan I just wanted to take a moment to appreciate your incredible work. Your unique teaching style is truly refreshing and inspiring. You have a remarkable ability to present complex topics in such an engaging and accessible way. Your creativity and passion for educating others shine through in every video. Thank you for making learning enjoyable and for sharing your expertise with us.
Very useful thank you
Thank you very much
This was very helpful 👍
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A really nice explanation. A hug from Brasil!
Love from India. Please share my channel link with your friends
Great video short and very clear!!!!!
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Great video, thank you so so much !
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Helps so much! Thank you
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Great work buddy keep this up
thanks a lot
Very helpful! Thank you!
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Thank you for this video!
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Great bro. Could you talk about why Lanthanum.Cl3.H20 (anhydrase) precipitate in room temperature water? (it has low solubility for sure). This question has nothing to do with protein solubility.
Finally got it thank you so much
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Wow that was a great explanation. it was very easy to understand. THANK YOU!
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Also subscribe to my other channel ruclips.net/channel/UC4IpyopsGWSjaPACNTZLuqg
THANK YOU FOR THIS!
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you done good
Amazing videoooo
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Very helpful 🙂👍
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@@animatedbiologywitharpan yes I just did
The video is really informative. 👍
Excellent video 😃
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Finally i understood..thanks
😅
loved it.. can you make a video on the change in protein conformation with the change in the ph of the solvent. Thank you!!!
I would try to make a video on that soon.......but please share my channel link with your friends and help me to reach big audience
Well explained sir
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Great video।।
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Great
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Thank you for this great video! Could you make a video about pI calculations of polypeptide chains?
so helpful and so concise, thanks so much!
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@@animatedbiologywitharpan for sure :)
So helpful thank you!
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It was awesome...
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