it's my 2nd year of medical school (my first if we didn't count the preparatory year )and I started to watch this channel it's really really great , I thought it has at least 5mil sub
You have good intentions, but your gonna be broke af after medschool for a good 4-5 years lol. I promise you. Maybe after 6-10 years after finishing medschool, you will make better money.
dude i must say your videos really are the best. Usually I can't sit through anything longer than 10 minutes so with longer videos skip to where i can see the entire board so i have all the data i need, but your explanations are so well done that i sat through the entire video, without dosing off despite the fact that it's 3 am. Thanks again for the amazing lessons man, keep up the good work!
This is the first channel I have subscribed to. For the first time, I have understood this lesson. I always just watch videos and move on, but this channel is a safe classroom, I can be confident in understanding my PhD program. Thank you
OMG!!!! You save my life for passing with high distinction in my first year Biomedical Science. Now, I am in the second year and I am really confident that I can pass with high distinction again. Thank you and god bless you!!
I just memorized the effects of inhibition for 4 years during pre-med without understanding no matter how much I tried reading about it. Now I am so happy to find your channel to understand the materials intuitively in med school LOL. DEFINITELY is gonna help me retain all of this long term now. I was hesitant at first seeing the first MM equation video's derivations but it makes perfect sense why you went through the trouble to explaining the whole thing. THANK YOU!
you are essentially saying the same thing....in the last video he talked about how at 1/2vmax [s]=km so the km is basically telling you the concentration of substrate required to reach vmax....he talked about how basically increasing km results in decreased substrate affinity i.e you need to increase [s] to reach vmax
I think something is off about the Competitive Inhibitor. If the Substrate Affinity goes up, the KM decreases, but as the Substrate Affinity goes down the KM increases. You explained that to beat the Competitive Inhibitor the Substrate Affinity must go up thus the KM will increase. No, I think the correct explanation is because the Competitive Inhibitor beat the Substrate's Affinity, the Substrate Affinity goes down hence the KM goes up. Because the effect of Competitive Inhibitor is KM goes up but Vmax unaffected. That's all from me, anyway amazing video! Really helps me understand difficult concepts!
yes, it's true the rise in Km means that there is a lower substrate affinity for the substrate and enzyme to make ES complex due to low km it takes for inhibitor as well as the high affinity for the inhibitor. So because enzyme has high affinity for the inhibitor it must be fixed. To fix it he has to increase the substrate concentration.
Its been really great watching your videos and you are making medical school so much easier for a lot of us out here. thank you so very much and God bless
Wow! You've been such a revelation to me. Thank you very much. These videos are obviously very easy to understand, and they give me so much confidence in Bich now.
careful! at 8:56! from what i understand, km has an INVERSE relationship with affinity. what's written on the board looks fine but when you say "km, or affinity, increases" is not!
The fact that aspirin is an irreversible inhibitor is actually why the dosage for aspirin as a bloodthinner is a lot lower than the dosage for aspirin as an anti-inflammatory drug, which I think is super interesting.
good explanation but I've got one question or one thing I don't understand. You said that when the substrate concentration will increase the Km will increase to and so the affinity. In the first part of the video you said that a high Km means that the enzyme has low substrate affinity. So how can the affinity become high in competitive inhibitor when the Km and substrate concentration increases?
Their website is up and running with all these videos and extensive notes on them, so I guess more extensive than the videos are already, I’m going to check it out to seek clarification
I think the idea, some enzymes have low affinity towards substrate so it has to reach high km in order to compete with the inhibitor and make the reaction
Could you please make a nucleotide synthesis video? I really miss a nice and well-explained biosynthesis and degradation of purines and pyrimidines on youtube... Why won't you be the first? :))) Pleeeaasseeee
Wow!!!!!! You are the best teacher i've ever seen😳😳😳😍👏👏👏👏 You are a huge help for online international students like me who do not have any teacher just pdfs and some imperfect videos! But YOU are the best, you are better than all videos and all PDFs! Please keep doing new videos as all Health science students deeply need you! Thank you for your being. I just started to watch all of your videos and i hope to watch more videos uploading on youtube from you🙏💯💯💯💯💯
Hey I have a question, at 9:00 you said that the Km or the affinity that the enzyme has for the substance is increasing but if the Km increases due to increase in substance concentration shouldn’t the affinity decrease; sorry if I have any writing errors, English is not my native language 😅
Wait so let me try to clarify this for my own sake: Km is the concentration of substrate required for the rate of the reaction to be half of Vmax. Competitive inhibitors DECREASE the Enzyme's substrate affinity (by binding in the active site). This results in a higher Km, because more substrate is required to outcompete the competitive inhibitors and raise the rate of the reaction to half of Vmax. Can anyone please make sure my logic is correct? Thanks!
Sir your lecture are brilliant and easy to understand.... and one thing I have request you to upload a snap of lecture you've done on board.... it is easy for revision also.....🙏
For the longest I thought the active site changes shape when the inhibitor bind to the allosteric site. Will it change shape? Or I got that totally wrong during my university lecture.
I've heard something about non competitive inhibitors changing the active site of the enzyme, but the substrate can still bind to active site although the active site had undergone changes because of the non competitive inhibitor, I'm confused 😕
This is amazing 🤩 Thank so much ninja nerd for making all these biochemistry lecture videos I really appreciate it 😊 could you replace my lectures on my biochemistry module?🥺
next time can you put eng subtitle in all of your lecture cause you talk very fast but i still get it.thank you for this amazing video and great explanations.its make me understand well
The subtitles/captions are automatically generated by RUclips. They are enabled for all of our videos, but RUclips decides if/when to produce them. Some Ninja Nerds have reported having great success in setting the playback speed to 0.75x in a effort to contain Zach's enthusiasm. See if that doesn't help while we wait for RUclips to do it's thing
This man single handedly training all of the world's future physicians.
If every one of Ninjas subscribers gave him $1, he would have $500,000. These lectures are better than any university class I've had.
I totally agree with you, Seeta! This is awesome work
More than one million $ now!
@@drjawad92 yaaa it's so awesome
I. m sure at the moment he. s making good money. He deserves everything!
1.7 million now
As a college student, I am broke. But! When I graduate med school, YOU dear Ninja Nerd Angels are getting a hefty chunk of change from me!
That's something on my bucket list too !
I can't even afford a cup of coffee! Ninja Nerds has been a blessing beyond words. They are also on my bucket list!!
it's my 2nd year of medical school (my first if we didn't count the preparatory year )and I started to watch this channel it's really really great , I thought it has at least 5mil sub
5 dollar now is better than good intentions later. Just saying ;) Skip that next beer and you're set.
You have good intentions, but your gonna be broke af after medschool for a good 4-5 years lol. I promise you. Maybe after 6-10 years after finishing medschool, you will make better money.
dude i must say your videos really are the best. Usually I can't sit through anything longer than 10 minutes so with longer videos skip to where i can see the entire board so i have all the data i need, but your explanations are so well done that i sat through the entire video, without dosing off despite the fact that it's 3 am. Thanks again for the amazing lessons man, keep up the good work!
same ong, damn thats a 2year old comment
This guy explains wayy better than my lecturer at the uni !
I don’t even know how to thank you for this. I have studied for hours without understanding and your 30 minute video solved the problem😭
This is the first channel I have subscribed to. For the first time, I have understood this lesson. I always just watch videos and move on, but this channel is a safe classroom, I can be confident in understanding my PhD program. Thank you
Your'e such an amazing teacher I'm so glad that I found this channel.
Lots of love to The Ninja Nerd Team from India
OMG!!!! You save my life for passing with high distinction in my first year Biomedical Science. Now, I am in the second year and I am really confident that I can pass with high distinction again. Thank you and god bless you!!
I just memorized the effects of inhibition for 4 years during pre-med without understanding no matter how much I tried reading about it.
Now I am so happy to find your channel to understand the materials intuitively in med school LOL. DEFINITELY is gonna help me retain all of this long term now.
I was hesitant at first seeing the first MM equation video's derivations but it makes perfect sense why you went through the trouble to explaining the whole thing. THANK YOU!
I want to be your friend
Literally me , but explanations are of another level
No book can explain like you!!!! Thank you soo much! I love your lessons :)
Thanks to you I passed biochemistry, really great work, keep doing it because you help a lot, thanks to you from the heart...!!!
Slight correction: for a competitive inhibitor higher km = decreased affinity for substrate
you are essentially saying the same thing....in the last video he talked about how at 1/2vmax [s]=km so the km is basically telling you the concentration of substrate required to reach vmax....he talked about how basically increasing km results in decreased substrate affinity i.e you need to increase [s] to reach vmax
thank you i was just wondering this!!
You’re an awesome teacher, your completely explain things that I’ve tried to get for months! Tank you so much... keep making videos :D
I think something is off about the Competitive Inhibitor. If the Substrate Affinity goes up, the KM decreases, but as the Substrate Affinity goes down the KM increases. You explained that to beat the Competitive Inhibitor the Substrate Affinity must go up thus the KM will increase. No, I think the correct explanation is because the Competitive Inhibitor beat the Substrate's Affinity, the Substrate Affinity goes down hence the KM goes up. Because the effect of Competitive Inhibitor is KM goes up but Vmax unaffected. That's all from me, anyway amazing video! Really helps me understand difficult concepts!
Why does the Km decrease of 'uncompetitive inhibition' then? How has the substrate infinity increased?
yes, it's true the rise in Km means that there is a lower substrate affinity for the substrate and enzyme to make ES complex due to low km it takes for inhibitor as well as the high affinity for the inhibitor. So because enzyme has high affinity for the inhibitor it must be fixed. To fix it he has to increase the substrate concentration.
Thnx ❣
I agree! but awesome video still...
This guy is great. Wish he had videos about everything I'm learning this semester because my professor sucks.
Its been really great watching your videos and you are making medical school so much easier for a lot of us out here.
thank you so very much and God bless
I learn more from your vids that I do in class
Wow! You've been such a revelation to me. Thank you very much. These videos are obviously very easy to understand, and they give me so much confidence in Bich now.
man if i pass my biochem final im gonna pray for you so much ill start appearing in your dreams
His lectures are so clear and easy to understand
careful! at 8:56! from what i understand, km has an INVERSE relationship with affinity. what's written on the board looks fine but when you say "km, or affinity, increases" is not!
Km increases , it increases the substrate(S) concentration and decreases the substrate affinity
Keep your mind clear 😂😂😂 cox😂😂 this guy has a sensational sense of humor
Your literally the best teacher ever! Never have I understood topics like this before your videos
I'm a french student who's not good at english but i understand everythink thank you for this video
These videos are addictive! 😊😊
Thank you for doing a thorough explanation. I appreciate that you included examples.
The fact that aspirin is an irreversible inhibitor is actually why the dosage for aspirin as a bloodthinner is a lot lower than the dosage for aspirin as an anti-inflammatory drug, which I think is super interesting.
I am currently for my pharmacy and poisons board exams and this video was really helpful. thank you. God bless you.
Thank you soo much professor, you have a gift of making anything understandable!!!
I love your videos they are so creative as I'm a visual learner, It helps a lot in Med School.
god bless this has been soooo helpful!! Thankyouuu
good explanation but I've got one question or one thing I don't understand. You said that when the substrate concentration will increase the Km will increase to and so the affinity. In the first part of the video you said that a high Km means that the enzyme has low substrate affinity. So how can the affinity become high in competitive inhibitor when the Km and substrate concentration increases?
This , i am confused as well
Their website is up and running with all these videos and extensive notes on them, so I guess more extensive than the videos are already, I’m going to check it out to seek clarification
I think the idea, some enzymes have low affinity towards substrate so it has to reach high km in order to compete with the inhibitor and make the reaction
You are helping humanity by teaching thanks so much
Of course, YES YES YES to every good comment ever made. Also, every time I hear the disclaimer after “COX,” I groan and think “whyyyyyyyyyyy????”
Lehninger takes 3 pages to explain this. you make life easier bro. love you
Sir plz make a video on metabolic acidosis and alkalosis...and buffer system
Could you please make a nucleotide synthesis video? I really miss a nice and well-explained biosynthesis and degradation of purines and pyrimidines on youtube... Why won't you be the first? :))) Pleeeaasseeee
Great explanations, very clear, very helpful. THANK YOU!
Dude, I just got an A in Biochem with the help of your explanations! I am eternally grateful! Greetings from Croatia ✌
much appreciation for taking the time to draw this all out :)
Really helpful, d best I've seen...tnk u Ninja
I can’t imagine that I could have learnt it better
Thanks doctor 💙💙💙💙🌎
You are the best " a learned man and cool guy "
Thanks for your efforts and all whom helping you to make these lecture clear and easy 🙏❤️😊❤️😊😊❤️
Am I student and wow...ur videos are really helpful....Was asking u guys to make a video on protein purification methods as well
One day I hope to be this good! Trying to be a prof 😭
Wow!!!!!! You are the best teacher i've ever seen😳😳😳😍👏👏👏👏 You are a huge help for online international students like me who do not have any teacher just pdfs and some imperfect videos! But YOU are the best, you are better than all videos and all PDFs! Please keep doing new videos as all Health science students deeply need you! Thank you for your being. I just started to watch all of your videos and i hope to watch more videos uploading on youtube from you🙏💯💯💯💯💯
You are the best tutor ever
Thanks this really helped with my studies, I study medicine in barbados
Very thankful … saved my marks in university
Ninja nerddddddddd❤❤❤❤❤. I don't even know how to say thank you. Thank God for u o 🙏
Hey I have a question, at 9:00 you said that the Km or the affinity that the enzyme has for the substance is increasing but if the Km increases due to increase in substance concentration shouldn’t the affinity decrease; sorry if I have any writing errors, English is not my native language 😅
Thank You so much. Only your video makes me understand of the concept. :)
These are the best writing markers I’ve seen, far better than Expo. Where can I get these??
Wait so let me try to clarify this for my own sake: Km is the concentration of substrate required for the rate of the reaction to be half of Vmax. Competitive inhibitors DECREASE the Enzyme's substrate affinity (by binding in the active site). This results in a higher Km, because more substrate is required to outcompete the competitive inhibitors and raise the rate of the reaction to half of Vmax. Can anyone please make sure my logic is correct? Thanks!
WOW!! You are the best!! Thank you so much for being a super duper awesome teacher!!!
Explanation really very clear.Thnks a lot
god that black marker looks like heaven to write with
Sir your lecture are brilliant and easy to understand.... and one thing I have request you to upload a snap of lecture you've done on board.... it is easy for revision also.....🙏
Are these helpful till today .. like is there any chance of change in today's concept compared to 7 yrs ago ??
Nope. The concepts are still the same. They're really helping me as a 1st year med student.
For the longest I thought the active site changes shape when the inhibitor bind to the allosteric site.
Will it change shape? Or I got that totally wrong during my university lecture.
very helpful
you’re the reason i’ll be able to go to med school one day
Brah, best lecture class ever😅
So , competitive , slows the reaction , and non competitive, stops it ?
Love it. Thanks, Z.
Thank you so much. I did great on test just by watching your videos
thank you for this video,really nice
Very interesting video!
Thank you Thank you Thank you!!!
18:39 in non competitive inhibition inhibitor bind to ES complex or in non competitive inhibition substrate bind to EI complex which is correct
Thanq for preking up the learning process😊.I have a doubt :disulfiram used to treat chronic alcoholism is an example of what type of inhibitor?
This is really helpful ! Thanks ❤
Very helpful video .thank you sir.
loved the Cox joke at the end
Thank you so much for this video!!
I've heard something about non competitive inhibitors changing the active site of the enzyme, but the substrate can still bind to active site although the active site had undergone changes because of the non competitive inhibitor, I'm confused 😕
thanks professor Ninja! 🙌🏼
fantastic teaching
Love this guy!
Thank you sooooooooo much sir. Respect from Pakistan 🇵🇰🇵🇰🇵🇰
Thank you Zach 😻
nice job this has helped me alot
Really helpful
Thankyou so much!
Спасибо за мотивацию учить английский
This is amazing 🤩 Thank so much ninja nerd for making all these biochemistry lecture videos I really appreciate it 😊 could you replace my lectures on my biochemistry module?🥺
So good!!!
Thank you!
Your brain❤
I'm a little bit confused on the relationship of Km=S both increasing here meanwhile it was vise versa in the other video, someone please explain
next time can you put eng subtitle in all of your lecture cause you talk very fast but i still get it.thank you for this amazing video and great explanations.its make me understand well
The subtitles/captions are automatically generated by RUclips. They are enabled for all of our videos, but RUclips decides if/when to produce them. Some Ninja Nerds have reported having great success in setting the playback speed to 0.75x in a effort to contain Zach's enthusiasm. See if that doesn't help while we wait for RUclips to do it's thing
Fantastic!
At the end of the non competitive inhabitation, how is the inhabitant released and the pocket fixed or removed?
i just wish my university could just let me pay my fee to him 😊
why no one has mention that Chad COX joke in the comment section🤣.. loving your videos sir
Woooow I just love ur lectures
amazing explanation 🤟
Person engulfed a toxin compound his life was saved by injecting substrate b , subsrate b is competive reversible or non competive reversible ?