PCR Method Video
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- Опубликовано: 12 мар 2020
- This lab video demonstrates how to perform a PCR (Polymerase Chain Reaction) in the laboratory setting.
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Tnq for explaining and demonstrating the pcr method very specifically. It really helped me so much. Im a biotech student doing my second year but due to covid i never went to my campus even once. I was lacking practical knowledge and worried about it so much but this video was really helpful
Loved this video. Just watched it an hour before my biotech lab.
Gotta love the energy in this video, man
Wow such a great n clear explanation with visualization. Thank you. It will be helpful for practical work.
I found this extremely useful. It's so simplified. Thank you for sharing.
I love this video. I just subscribed to your channel. Very informative and interesting.
Thank you for this amazing video. Excellent explanation and visualization.
This video was very helpful!
Phenomenal explanations and techniques.
GREAT. YOUR PRONOUNS VERY WELL AND I UNDERSTAND YOU
Thank you so much for a great video!❤❤❤
helping me push my grades up thnx
it really helps me. tysm
So helpful, thank you.
Wowww, very kind of you, it's make me clear about PCR Method
Wonderful explanation 🎉
This is helping me study for my finals but I couldn't help but notice that the tip case was open the whole time, thats a little risky
Gotta keep 'er movin
That was great thank you
Thank you, a very informative video 👍
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so great 🙏
This is Gold! Thank you!!!!
Glad it was helpful!
thank you so much
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Good job.
thank u so much
Thanks
Could you show us how to perform cell culture of human cells
Hi guys. There's something I don't understand. Where did he put the DNA sample to be amplified?
so what about the results? what did you learn from this work
Great
Thank you so much for sharing this video. I am curious do you think if you were processing multiple PCR test at the same time that a viral particles can travel from one specimen sample to another or is it obviously best practice to sanitize everything before you process another sample. What do you think the industry is doing nowadays to prevent contamination from one container to another when they’re doing mass testing for covid 19?
Huge amounts of one-time-use plastic brotha.
Maybe I'm misunderstanding your question but each tube had a locking plastic lid so I can't imagine any particles being free in the machine.
@Jeremy Marsh I could have posed the question with an example. let's say person A has viral shedding occurring. They sneezed in their car several times before arriving to the testing facility. They pull up roll down the window with their air in car spreading those viral particles all over the place. The person who takes the sample now has the virual particles all over their person because they reached their arm in the car of individual A. Throughout the day the person who takes the sample may not knowingly be dropping the particles off their ppe into sample containers. @ Jeremy Marsh do you know if they replace their PPE including the gown they wear after taking every sample from a person in a car? If I got tested I may have or not noticed they did not do that Change their gowns after taking someone's sample. I almost felt like for all I know this person taking my sample could be dropping the viral particles in my car. I am ignorant of how all this works but from my understanding, the viral particles from people shedding comes out in the billions, and in a car with the heater cranked it's just flying all over the car when people shedding cough or sneeze in their cars before coming to get tested. This is one of many examples of how containers or even bins containing samples can get contaminated.
@@quantummandavid I would think if a larger than normal positive rate was traced to a certain testing site, they would have to look at the collection technique. Furthermore, the sites I've been to has the patient self-collect the sample and seal the test tube themselves, so the probability of contamination is negligible if not nonexistent. I'm an ER nurse and have collected and sent several hundreds if not thousands of tests in the past 2+ years, and the negative rate is more than I might expect, and trust me, I go allll the way into the nose!
@David espinoza If I’m understanding your question, I believe that after a pcr, one would run a gel electrophoresis- which includes a negative test tube for checking contamination besides reassurance that there’s indeed genetic material in samples. I hope this helps
You keep opening the vials from the ice bath with one hand, you're contaminating them all.
Initial denaturation was 5 minutes? were you analyzing eukaryotic genomic DNA??
is it compulsory to start the pcr programm with the initial denaturation step (95/5min)?
Great question! No, holding this temperature for 5 min is probably not really compulsory- most of the DNA is probably denatured before 5 min. We often hold this first step for 5 min just to ensure that as much DNA is single-stranded, which should maximum how much PCR product we make after 30 cycles. But the length of time of this initial step is a variable we can change to optimize the yield of the reaction. The time duration of this step can depend on the other experimental conditions: the the template and primer DNA sequences; the type of enzyme being used; and the salt concentrations of buffer. Using 5 minutes often works, but we can change it to optimize the reaction if it doesn't work the first time. But the temperature is relatively compulsory. PCR requires the denaturation step (initial and otherwise) to be programmed at a high temperature, commonly between 94-98°C.
@@LabXchange Very clear! Thank you for your time :)
Where can I get one of these PCR machines? And how much do they cost?
biorad
Are you adding everything into one tube?
No
❤️❤️❤️❤️
One thing I don't understand as a high school student is when they'll design complementary primers aren't they already aware of the sequence of the DNA they are amplifying? because primer is complementary to the strands so when they'll design them they should know the sequence of that DNA right?
yes, they need to check genebank of what DNA sequence they want to amplify
Right so the whole point isn't to know what the sequence is (that would be sequencing). It's to make many copies for further testing.
Primers are synthetic RNA molecules right??? Not DNA?
Such a good question. In the cell, yes, you are totally right: primers are made as short RNA molecules.
However, when we do it in a test tube, we typically use DNA primers (which have several benefits, such as increased stability.)
How did you pick up 1ng!!! Thats 0.001ug!
so what does all this mean in ENGLISH !!
🤣🤣
PCR was not developes for diagnosis and should never be used for this