In this video, Monique demonstrates how to perform a restriction enzyme digest in the lab. View this video (and more like it) on LabXchange: www.labxchange.org/
Hi LabXchange, I just want to ask something about this lesson. and here's the question why do we need to use vector plasmid and insert plasmid in this reaction? and what's the differences between vector plasmid and insert plasmid?
Great question! Basically, you have a vector (the majority of the DNA needed to express proteins) and then the insert (the DNA that codes for the gene that expresses the protein you actually care about.) Sometimes, the insert DNA comes from a linear fragment. But in this case you're cutting it out of a circular plasmid. Does this help?
@@paolamedina9934 good question. The plasmid concentration in this experiment is 0.25 ug/uL ("micrograms per microliter"). Since we want to use 1 ug of plasmid in this reaction, we used 4 uL of plasmid DNA ( 0.25 ug/uL x 4 uL = 1 ug).
Thank you for your video this was simple and concise. Helped me better understand and prepare for my own restriction enzyme digest.
Great video!
Another brilliant video. I couldn't help but get distracted by the beautiful gap toothed lady in the video.
Thanks for the video
Thank you for your video. I just wanna ask if i can add the enzyme before adding the DNA vector in the tube?
Hi LabXchange, I just want to ask something about this lesson. and here's the question
why do we need to use vector plasmid and insert plasmid in this reaction? and what's the differences between vector plasmid and insert plasmid?
Great question! Basically, you have a vector (the majority of the DNA needed to express proteins) and then the insert (the DNA that codes for the gene that expresses the protein you actually care about.) Sometimes, the insert DNA comes from a linear fragment. But in this case you're cutting it out of a circular plasmid. Does this help?
@@LabXchange Yes, it does! thank you so much LabXchange :))
What was your plasmid DNA concentration? :o
Or why did you put 4 uL of plasmid DNA. I don´t understand :(
@@paolamedina9934 good question. The plasmid concentration in this experiment is 0.25 ug/uL ("micrograms per microliter"). Since we want to use 1 ug of plasmid in this reaction, we used 4 uL of plasmid DNA ( 0.25 ug/uL x 4 uL = 1 ug).
@@LabXchange thanks❤️❤️❤️
Thanks for the video. Would you mind sharing your your contacts eg email?
You can find our email address in our channel's About section. Thanks!