Introduction to PCR || Steps and Applications of Polymerase Chain Reaction (PCR)

PCR - Polymerase Chain Reaction - This is the video on Definition of PCR, Requirements, Principle, Steps and Applications of PCR with NEET PG MCQs.
It contains -
History of PCR - 00:33 minutes
Definition of PCR - 00:51 minutes
Requirements of PCR - 01:51 minutes
Principle of PCR - 02:36 minutes
Steps of PCR - 03:26 minutes
Denaturation - 03:51 minutes
Renaturation (annealing) - 04:54 minutes
Polymerization (synthesis) - 05:48 minutes
Applications of PCR - 08:26 minutes
NEET PG MCQs - 11:16 minutes
To download lecture notes -
www.biochemistrybasics.com/20...
Subscribe my channel from - / biochemistrybasicsbydr...
For more intersting stuffs related to biochemistry visit -
www.biochemistrybasics.com/
Follow me
On Twitter - / dr_biochemistry
On Instagram - / biochemisrybasics
On Facebook - / amit.v.m
#PCR #PolymeraseChainReaction

[@lokeshalokesha8646]

Thank you so much sir..... 🙏🙏🙏 It's very helpful to us....hard work from your side sir

[@Biochemistrybasics]

Thanks dear for your kind words. Please spare a few minutes and do spread the word about the channel among your batchmates and friends

[@sekhareranki9278]

Your teaching is impressive and attractive sir !
Thanking you for delivering this topic simply 🙏

[@Biochemistrybasics]

Thanks a lot dear

[@mananshah3197]

I'm not being able to download notes sir, kindly help :)

[@KS-lm4oc]

Thank you sir .... it's really very informative in short words..... Please make video on Southern hybridization analysis and Dot blot hybridization analysis

[@Biochemistrybasics]

Sure

[@user-cw2fl9np2b]

Thank you Dr. for the nice explaination .I am a medtech student and this helps me a lot.

[@Biochemistrybasics]

Glad to hear that

[@musikaaffection8570]

Thank you SO much sir

[@Biochemistrybasics]

Most welcome dear

[@SandeepKumar-io3wv]

Mst zabardst video on PCR

[@Biochemistrybasics]

Thanks a lot

[@chintu1482]

Immunochemistry pe ek lecture bana do na sir....hybridoma technique bhi

[@Biochemistrybasics]

Sure will do

[@khamkheali]

Sir please discuss water oxigen perimeter

[@Biochemistrybasics]

Okay dear

[@user-vw3bm7qh7x]

I did not understand how the primers fix in the DNA

[@skillmedica]

Sir are notes of this vedio free in app?

[@Biochemistrybasics]

Few are free and rest are paid

[@satyanarayankumar9148]

Sir please reply me...major difference b/w the primers and probes

[@Biochemistrybasics]

Probes are the single-stranded nucleic acid employed in the hybridization while primers are used in the amplification
Differences between probe vs primer:
The length:
One of the crucial factor in any of the reaction is the length of the nucleic acid molecule used as a primer or probe.
The primer must be shorter ranging from 15 to 25 nucleotides while the probes are longer; ranging from 100 to 1000 or sometimes 10,000 nucleotides in length
Function:
The primary function of the probe whether it is DNA or RNA probe is to detect the presence or absence of target nucleic acid present in a sample.
While the function of a primer or a DNA primer is to provide a free 3’ end for Taq DNA polymerase to synthesise new DNA strand.
The probe binds at our target location whereas the primer binds from where the amplification will starts.
Probes are used for the detection through hybridization thus, there must be a colour molecule attached to it.
The probes are generally labelled with the radiolabelled or non-labelled compounds while the primers are not labelled.

[@satyanarayankumar9148]

So... northern blotting and southern blotting - probes
Pcr--- primers...
This is the final conclusion ..thank you so much sir...

[@Biochemistrybasics]

@@satyanarayankumar9148 Yes