Thnkuuu so much sir.... I searched everywhere but I was not able to get it... Thnkuuu so much... Plz make a video for quantity of each component... Thnkuuu so much
How important is working in sterile conditions while preparing the PCR Mastermix etc.? Working on ice in sterile conditions could get a little tricky I suppose?
+Libe Jenkins first we add the larger volume, it can be buffer or water, depending on the reaction. Then we add the lowest volume, and the reason is because in case of lower volume such as the DNA polymerase, it's viscous, so there will be a lift over inside the tip after we add the desired volume into the reaction mixture, If we add the buffer and the water before the DNA polymerase, we can the desired volume of the DNA polymerase and then washing the left over inside the tip by mixing the tip up and down with the buffer and water in the reaction mixture.
this good for me useful and thanks for you all best wishes for you can do anything just like me aanything just fun like mthanks lab givingf this progrmm
I liked your detailed explanations and why reagents were being added. Thanks
Thnkuuu so much sir.... I searched everywhere but I was not able to get it... Thnkuuu so much... Plz make a video for quantity of each component... Thnkuuu so much
4:42 -4:48 That awkward moment xD
thanks. very knowledgeable demonstration..
cool eye protector is that an XL... made the video fun...
How important is working in sterile conditions while preparing the PCR Mastermix etc.? Working on ice in sterile conditions could get a little tricky I suppose?
Thanks for this useful video..a.humungous thank you
As far as im concern the water should be added first, then the lowest volume...
سلام
+EmiLy MenHer Why? because the components are acid?
+Libe Jenkins first we add the larger volume, it can be buffer or water, depending on the reaction. Then we add the lowest volume, and the reason is because in case of lower volume such as the DNA polymerase, it's viscous, so there will be a lift over inside the tip after we add the desired volume into the reaction mixture, If we add the buffer and the water before the DNA polymerase, we can the desired volume of the DNA polymerase and then washing the left over inside the tip by mixing the tip up and down with the buffer and water in the reaction mixture.
this good for me useful and thanks for you all best wishes for you can do anything just like me aanything just fun like mthanks lab givingf this progrmm
At 1:12 the technican throw the pipette.
She did.
Yes. Yes she did.
Lance C
The demo is unclear,but appreciated.
Thank you very much, sir
Is anyone able to tell me how to cite this video in APA?
THANKS A LOT
Thank you so much .
very very useful... than you.....
nice exp.
thanks alot but whats the purpose of put our sample in ice ?
good job
Film intéressant (en anglais) sur la PCR
She looks really amused...