How to set up a PCR

In this film, Dr Cath Arnold from the Health Protection Agency demonstrates how to set up a Polymerase Chain Reaction (PCR).
For a transcript of this film, paste the below link into your browser:
www.hinxton.wellcome.ac.uk/adv...

[@hraqhraq]

5:37 Don't forget to leave you hair uncovered, to get some of your technician's hair samples in the tubes for better buffering results and to confuse the hell of the Doctor when results shows that child's mother is not his mother but the mother of the technician!!!

[@shammasantaagata798]

Hi! I just want to ask, can I use your video in the project that I am making? Thanks!

[@immaculatemphepo2062]

The video is so helpful, thank you

[@mertdurmus9536]

this video is so helpful thank you

[@rubelrana7516]

Very important... Thanks

[@noman12310]

Thank you

[@jorgecespedesverona6913]

buen vídeo
saludos

[@monmicfer]

I could be very wrong, but for a 50 microliter mix of 7 reactions you should have 350 microliters, not 340 microliters. Water should be added until 343 microliters, not 333 microliters.

[@edubielalpizar4391]

you're right. I also realised that suddenly my maths did not match... was like why... and then I noticed they suddently did what you mentioned. which is a mistake since in the beginning they indeed mentioned the final volumen (for 7 reactions) was 350 ul (therefore 175 ul from the master mix aso...).good to see I was not the only one.

[@shuaicao1438]

nice job!!!!!

[@TheAu4812555]

Thanks

[@gustiayusari9691]

Do you have the reference of the ideal room?

[@LaboratoireLAMC]

Thank you for the video

[@md.shirajummonir1654]

Thnks

[@siddharthsrivastava8652]

Video on Calculations that go into assembling such PCR reaction
ruclips.net/video/gNrNfjHO4cA/видео.html