Addgene does not regularly monitor comments posted here, so we may not see your question immediately. We’d be happy to answer any questions sent to help@addgene.org as soon as possible. Please include the name of the video along with any questions so our support team can help. Thanks!
Overall very helpful video I appreciate the time and effort you put into explaining the stages of annealing and denaturing-- very helpful. Some more details on the ingredients list, especially explaining mM and buffers, and how they apply to the overall chemistry of PCR, would be helpful, thank you so much!
Thanks for the feedback! We try to pack as much info into videos as possible, but sometimes we can't cover everything. But we do have a written PCR protocol that digs into some more details: www.addgene.org/protocols/pcr/
It looks like you're talking about qPCR? This guide has some more information on the cycle threshold for qPCR www.thermofisher.com/us/en/home/life-science/pcr/real-time-pcr/real-time-pcr-learning-center/real-time-pcr-basics/real-time-pcr-understanding-ct.html
Thanks for the feedback! We try to pack each video with as much information as possible, but some things just can't make it into the video. For a more in-depth look, you can check out our written protocol here: www.addgene.org/protocols/pcr/
Thanks for the question. Magnesium chloride is already included in the 10X Taq buffer, but some PCR reactions may require more magnesium chloride to improve DNA amplification. Therefore, adding more magnesium chloride to a PCR reaction that is not working might improve your results. However, excess magnesium chloride can decrease the binding specificity of your primers, so we suggest trying a PCR reaction under standard conditions first.
Really amazing, I have doubt , if target is amplified remaining PCR components where it goes, another one doubt during extension , how it sharply terminate at target site - please explain
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Addgene does not regularly monitor comments posted here, so we may not see your question immediately. We’d be happy to answer any questions sent to help@addgene.org as soon as possible. Please include the name of the video along with any questions so our support team can help. Thanks!
Those funny moments are amazing... great job guys keep up the good work
All protocols are given complete information, Thankyou
I have attained better understanding of elution and PCR after watching your video. Thank you so much.
thank you for delivering clear information about the whole pcr proceduee
thank you for clarification.hats off madam
Very constructive videos! Please I need to get PCR-RFLP steeps?
Absolutely fantastic
Amazing presentation
Overall very helpful video I appreciate the time and effort you put into explaining the stages of annealing and denaturing-- very helpful. Some more details on the ingredients list, especially explaining mM and buffers, and how they apply to the overall chemistry of PCR, would be helpful, thank you so much!
Thanks for the feedback! We try to pack as much info into videos as possible, but sometimes we can't cover everything. But we do have a written PCR protocol that digs into some more details: www.addgene.org/protocols/pcr/
Brilliant! Thank you so much!❤
Veryclear explication thank you!!
Plz share info about genomics .. Proteomics... Transcriptomics...
THANK YOU VERY MUCH IT WAS VERY HELPFUL VIDEO
❤️I love addgene's website
Really wonderful video 😍😍
can you explain me in detail about threshold value ,limitation of pcr ...
It looks like you're talking about qPCR? This guide has some more information on the cycle threshold for qPCR www.thermofisher.com/us/en/home/life-science/pcr/real-time-pcr/real-time-pcr-learning-center/real-time-pcr-basics/real-time-pcr-understanding-ct.html
thank you
Superb!
Thanks❤
PCR baby!! WOOHOOOO!!!!!
Nice
Very cool
Why we but the componant on ice at the beginning?
very nice video. I like the solutions to the problems
Content were well arrange but less details kindly add some more details our all👍👍❤️
Thanks for the feedback! We try to pack each video with as much information as possible, but some things just can't make it into the video. For a more in-depth look, you can check out our written protocol here: www.addgene.org/protocols/pcr/
Great maam!...
All want in life is the PCR machines to be covered with cloth that can dramatically be removed with a soundbite cheering me on during setup.
V good
Lovely
Why not add mgcl2 initially?
Thanks for the question. Magnesium chloride is already included in the 10X Taq buffer, but some PCR reactions may require more magnesium chloride to improve DNA amplification. Therefore, adding more magnesium chloride to a PCR reaction that is not working might improve your results. However, excess magnesium chloride can decrease the binding specificity of your primers, so we suggest trying a PCR reaction under standard conditions first.
Anyone know this method is conventional or real time PCR?
Hello! It's conventional (end point) PCR.
Love
Really amazing, I have doubt , if target is amplified remaining PCR components where it goes, another one doubt during extension , how it sharply terminate at target site - please explain
Tanx
good ass video
3:27