Competitive ELISA Kit Operation Guide - Elabscience

This is a video about competitive ELISA kit operation. The micro ELISA plate provided in this kit has been pre-coated with a specific antigen. During the reaction, the antigen in samples or Standard competes with a fixed amount of the antigen on the solid phase supporter for sites on a biotinylated detection antibody specific for antigen. Excess conjugate and unbound sample or standard are washed from the plate, and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated.
During the reaction, the antigen in samples or Standard competes with a fixed amount of the antigen on the solid phase supporter for sites on a biotinylated detection antibody specific for antigen. Excess conjugate and unbound sample or standard are washed from the plate, and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated.
The OD value is inversely proportional to the concentration of antigen. You can calculate the concentration of antigen in the samples by comparing the O.D. of the samples to the standard curve.
Check your desired ELISA assay kits with citations here:
www.elabscience.com/Products-...