Pipetting with precision - how to minimise experimental error

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  • Опубликовано: 21 ноя 2024

Комментарии • 31

  • @salah-dred8576
    @salah-dred8576 Год назад +6

    This content is what a biologist need, practical, clear and right to the point. Thank you my friend !

  • @Eve.Daniels
    @Eve.Daniels Год назад +4

    OMG where was this video two years ago 🥲 I learned to pipette from diagrams in old textbooks during lockdown. Didn't even THINK to look on RUclips until a year later 😭

  • @Weatherman4Eva
    @Weatherman4Eva Год назад +5

    Thank you very much for this! I'm applying for a job as a biochemistry lab assistant and it's been quite a few years since college so I'm brushing back up on the fundamentals

  • @chubbyobasi814
    @chubbyobasi814 Год назад +1

    Thank you for this video. I really appreciate your honesty. As simple as it my seem, the errors from pipetting are shocking. One thing to add tho when dispelling, after the first stop an angle should be made to at least touch the side of the tube before pushing to the second stop(blow off). Helps in accuracy involving smaller volumes. Thanks again❤

  • @kiklly
    @kiklly 2 года назад

    Thank you so much for this video.

  • @ervincui1914
    @ervincui1914 Год назад +1

    When I am depressing the pipette, I go to the first stop and then the second stop and pull away fast to avoid any little bit of liquid adhereing to the inside of the tip. However, there’s always a little bit left and when I aspirate again it is visibly more than the first time I aspirate with a new tip. How do I make sure I’m pipetting all of the volume out consistently?

    • @BioLabCollective
      @BioLabCollective  Год назад +2

      Sounds like you are pressing too hard (slightly past the first stop) when drawing up liquid, hence the additional air or liquid in your pipette. Could also be the consistency of the speed at which you press the plunger - needs to be slow and smooth, not sudden or jerky. Check this video out for the most common mistakes: ruclips.net/video/a3KGW1uDllo/видео.html

  • @theelusivememe1772
    @theelusivememe1772 Год назад +2

    Okay so the first stop is for drawing up liquid and you only go to the second stop when dispensing. But am i suppose to press the second stop while submerged in the liquid cause i always get bubbles, or do you only press to the second stop unsubmerged in the liquid?

    • @BioLabCollective
      @BioLabCollective  Год назад

      Generally we don’t dispense liquid with the pipette submerged in liquid? Pipette to the side or the opening of the tube and bottle to minimise disruptions on the liquid you are pipetting into

  • @kimmytran9973
    @kimmytran9973 2 года назад

    Thanks for sharing! When dispensing liquid, I release at the first stop then second stop, but sometimes there’s liquid remaining. Do you have any idea on why this happens? Should I press it up and down to dispense the last liquid?

    • @BioLabCollective
      @BioLabCollective  2 года назад

      Sounds like you’ve pushed past the first stop when drawing up liquid at the start? Too much liquid, otherwise there shouldn’t be any left over. Sure dispense all of it, but you should practice your technique more using the tips we talk about in the video.

    • @kimmytran9973
      @kimmytran9973 2 года назад

      @@BioLabCollective thanks I will closely watch it next time!

    • @kimmytran9973
      @kimmytran9973 2 года назад

      @@BioLabCollective thanks for the tips! My technique has improved alot. Another question- for positive displacement pipette instead of air displacement pipettes like these , do positive displacement pipettes have first and second stop? I have quite a bit of trouble using them not sure if I use them correctly.

    • @BioLabCollective
      @BioLabCollective  2 года назад

      Yes they should have first and second stops too - depending how how they’re tuned you may not need to push to the second stop to dispense all the liquid? The second stop may just be reserved for ejecting the tip

  • @zbigniewrozynek5121
    @zbigniewrozynek5121 2 года назад

    What about the reverse pipetting? The standard pipetting technique (that you present) is not good for some liquids.

    • @BioLabCollective
      @BioLabCollective  2 года назад +1

      Reverse pipetting is very useful, but not my first recommendation if you’re new to pipetting. It’s hard enough to master the standard technique for most people. Maybe a topic for a new video!

  • @gkum6067
    @gkum6067 Год назад

    How to do avoid bubbles while pipetting and if bubbles come how to pipette the sample from those bubbles.

    • @BioLabCollective
      @BioLabCollective  Год назад +2

      Most common problem is pressing down too quickly when you are drawing up the liquid. It takes practice to do this smoothly - will cover more tips in a follow up video

  • @skazka3789
    @skazka3789 Год назад

    When I'm pipetting smaller volumes like 2 uL, often the drop does not want to leave the pipette and is stuck to the tip

    • @BioLabCollective
      @BioLabCollective  Год назад

      Pre-wetting the pipette tip before aspirating any liquid may help? Depends on the viscosity of the liquid too

    • @skazka3789
      @skazka3789 Год назад

      @@BioLabCollective thanks for your advice. btw i watched another vid where u say u wake up at 5 am. can i ask what time you sleep lol

    • @BioLabCollective
      @BioLabCollective  Год назад

      @@skazka3789thankfully 5am wakeup is not everyday! I don't get to sleep much unfortunately, hoping to change that in the new year.

  • @jaynethmi
    @jaynethmi Год назад

    When i used 2nd stop produce a air bubble. What is the reason for tht?

    • @BioLabCollective
      @BioLabCollective  Год назад +1

      You can try immersing the pipette tip in the liquid before you draw up any liquid. The speed at which you push down on the plunger can also affect bubbles?

    • @jaynethmi
      @jaynethmi Год назад

      @@BioLabCollective thank you 🙂

  • @Oniku-niginigi
    @Oniku-niginigi 6 месяцев назад

    3:44