I like this video for its content, presentation and the quality of the picture/sound. Great piece of work in online education. Gratefully appreciated....Thanks.
tip #1. In my last employment, they never pre-wet tips. i was the only one who did. my results were higher than the rest of the group. i was reprimanded for not following sop.
Its a good wholesome video. Good to take two vials for practicing pippetting from one vial to another. The veritcak smooth plunging asoursying and dispensing. Repeatedly. It takes 1-3 months yo get used to get the skill to settle in your innner being. Using calibration and weinging machince pipetting can be checked. Maybe will edit this after sometime ~ on calbration.
the eppendorf electronic 12-ganga and 8-gang pipette can accommodate 200-uL and 300-uL tips. can i use 200-uL tips when working with smaller volumes? or is it explicitly forbidden?
Thank you for watching the 10 tips video series and for your question. Traditionally, the terms pre-rinsing and pre-wetting refer to the same process however in practice, we have observed them to be quite different. Below I’ve outlined the steps to illustrate the technique differences, which primarily center around removing the tip from the sample solution. Pre-Rinsing: press the plunger to first stop, place the pipette tip in sample solution, leave the tip in the sample solution and exercise plunger up and down several times. Pre-Wetting: press the plunger to first stop, place the pipette tip in sample solution, aspirate sample, remove tip from sample solution, press plunger to second stop to fully dispense sample against side of receptacle. Repeat steps at least 3 times. I hope this information is helpful. Thank you again for reaching out.
@@SH-fz5pt You are welcome. We thought you might find these additional resources helpful; ten tips to improve your pipetting technique www.artel.co/learning_center/10-tips-to-improve-your-pipetting-technique/ and pipetting viscous solutions best practices www.artel.co/learning_center/pipetting-viscous-solutions/.
![[DRAGON BALL LEGENDS REVEALS ＆ STUFF] LEGENDS FESTIVAL 2024 Special Edition Part 1](http://i.ytimg.com/vi/mV5bGIswTtQ/mqdefault.jpg)
Did he really have to say some variation of "improving your pipetting technique will improve your results" before giving each tip?!
I like this video for its content, presentation and the quality of the picture/sound. Great piece of work in online education. Gratefully appreciated....Thanks.
tip #1. In my last employment, they never pre-wet tips. i was the only one who did. my results were higher than the rest of the group. i was reprimanded for not following sop.
Its a good wholesome video. Good to take two vials for practicing pippetting from one vial to another. The veritcak smooth plunging asoursying and dispensing. Repeatedly. It takes 1-3 months yo get used to get the skill to settle in your innner being. Using calibration and weinging machince pipetting can be checked.
Maybe will edit this after sometime ~ on calbration.
This was fabulous, thank you.
IN SOME VIDEO SAYS PRE WETTING THE TIPS DEPENDS ON SOP BUT NOT ALL
Useful tips, thank you!!!!
can i get that "take home massage" one more time ...thanks!
Great info sir thx
When you release the Liquid do you go directly to the 2nd stop
Deliver with the first stop and blow the remaining liquid out completely with the second stop.
Thank you a million times.
Great! I was looking for this kind of video! I love you! It's perfect!
Highly useful tips. Thank you so much
the eppendorf electronic 12-ganga and 8-gang pipette can accommodate 200-uL and 300-uL tips. can i use 200-uL tips when working with smaller volumes? or is it explicitly forbidden?
Does pre-rinse and pre-wet means the same thing?
Thank you for watching the 10 tips video series and for your question. Traditionally, the terms pre-rinsing and pre-wetting refer to the same process however in practice, we have observed them to be quite different. Below I’ve outlined the steps to illustrate the technique differences, which primarily center around removing the tip from the sample solution.
Pre-Rinsing: press the plunger to first stop, place the pipette tip in sample solution, leave the tip in the sample solution and exercise plunger up and down several times.
Pre-Wetting: press the plunger to first stop, place the pipette tip in sample solution, aspirate sample, remove tip from sample solution, press plunger to second stop to fully dispense sample against side of receptacle. Repeat steps at least 3 times.
I hope this information is helpful. Thank you again for reaching out.
@@ARTEL10Tips Thank you for taking the time to answer my question!
@@SH-fz5pt You are welcome. We thought you might find these additional resources helpful; ten tips to improve your pipetting technique www.artel.co/learning_center/10-tips-to-improve-your-pipetting-technique/ and pipetting viscous solutions best practices www.artel.co/learning_center/pipetting-viscous-solutions/.
Thank you so much!
never got teached to pre-wet the pipette!
Great
How to
NEVER dispense back into the sample container, that's horrendous advice!
500th like!