We highly recommend running each standard and sample in duplicate or triplicate. If running duplicates, you will have two wells for each standard and sample. If running in triplicate, you will have three wells for each standard and sample.
RayBiotech's sandwich ELISA kits are pre-coated 96-well strip plates. If you would like to split up your plate testing, each strip includes 8 wells so you can easily split it up in groupings of 8.
We admit our microphone could be better. Thank you for bearing with us as we pilot this project. Did you find the visuals and subtitles helpful? We are open to hearing what more we could do to help demonstrate this technique. Feel free to email info@raybiotech.com for more video requests.
You may touch the ends of the well strips and the sides of the plate with gloved hands. To maintain a sterile working solution avoid touching the individual wells, even if you have gloves on. You can run liquid down the sides of the wells with a sterile pipette tip.
Whether you need a laminar flow hood for your ELISA experiment depends on various factors. ELISA involves handling sensitive samples and requires aseptic conditions to prevent contamination. If you're working with infectious agents, cell cultures, or potentially hazardous substances, a laminar flow hood is essential to maintain a sterile environment and protect both the experiment and the researcher. Additionally, a laminar flow hood aids in preventing cross-contamination between samples and ensures accurate results. However, if your ELISA experiment involves only non-infectious and non-hazardous materials, a well-maintained and clean laboratory bench typically suffices. This video assumes a well maintained and clean laboratory environment is used to run the experiment and does not demonstrate handling infectious material. Ultimately, the decision should consider the experiment's complexity, potential risks, and adherence to best laboratory practices.
For our ELISA kits, we use 96 well plates and a volume incubation of 100uL. If referring to running 24 wells in 3 strips, the conditions are the same. If you're referring to a full 24 well plate then development and use can be accomplished with 1mL per well volume.
Pipette washing is a common practice and a widely acceptabled way to remove liquid. Many labs will use a clean pipette tip hooked up to a vacuum to suction out liquid. When doing so, please tilt the plate at an angle and be careful not to scrape the bottom of the well with the pipette tip. It's best if an experienced team member demonstrates this technique prior to a new user attempting it for the first time. If this is not possible, then it is best to blot on tissue paper to ensure all liquid is removed. The goal is to remove all liquid while maintaining the integrity of each well during the removal step.
![IQELISA™ [Good Laboratory Practice Guide]](http://i.ytimg.com/vi/e0z5VWkTB-w/mqdefault.jpg)
![IQELISA™ [Good Laboratory Practice Guide]](/img/tr.png)
Great tutorial, thanks. Now, let's get into the lab, asap.
Awesome presentation thanks a lot there dear madam ❤❤
Hello! Do we have to use double well for stds and samples? or can I just use 1 for each
We highly recommend running each standard and sample in duplicate or triplicate. If running duplicates, you will have two wells for each standard and sample. If running in triplicate, you will have three wells for each standard and sample.
thank you, it's informative, but the audio quality is just terrible
Glad it was helpful! :) We appreciate your feedback and will take it into consideration.
Thanks for this video.
Glad you like it :)
Mam can you give any tips to cut the wells perfectly without any mistakes
RayBiotech's sandwich ELISA kits are pre-coated 96-well strip plates. If you would like to split up your plate testing, each strip includes 8 wells so you can easily split it up in groupings of 8.
Use mic please
We admit our microphone could be better. Thank you for bearing with us as we pilot this project. Did you find the visuals and subtitles helpful? We are open to hearing what more we could do to help demonstrate this technique. Feel free to email info@raybiotech.com for more video requests.
Can we touch the well when pipetting?
You may touch the ends of the well strips and the sides of the plate with gloved hands. To maintain a sterile working solution avoid touching the individual wells, even if you have gloves on. You can run liquid down the sides of the wells with a sterile pipette tip.
@@RayBiotech Is it direct or indirect or compittive or sandwich elisa? pleasetell me.
@@408_mohitpanchal2 sandwich elisa
Shouldn't it have done in a sterile conditions under a laminar flow?
Whether you need a laminar flow hood for your ELISA experiment depends on various factors. ELISA involves handling sensitive samples and requires aseptic conditions to prevent contamination. If you're working with infectious agents, cell cultures, or potentially hazardous substances, a laminar flow hood is essential to maintain a sterile environment and protect both the experiment and the researcher. Additionally, a laminar flow hood aids in preventing cross-contamination between samples and ensures accurate results. However, if your ELISA experiment involves only non-infectious and non-hazardous materials, a well-maintained and clean laboratory bench typically suffices. This video assumes a well maintained and clean laboratory environment is used to run the experiment and does not demonstrate handling infectious material. Ultimately, the decision should consider the experiment's complexity, potential risks, and adherence to best laboratory practices.
tnx but what about 24 -well plate filling format sample, blank and standard
For our ELISA kits, we use 96 well plates and a volume incubation of 100uL. If referring to running 24 wells in 3 strips, the conditions are the same. If you're referring to a full 24 well plate then development and use can be accomplished with 1mL per well volume.
Is it direct or indirect or compittive or sandwich elisa? pleasetell me.
This is a sandwich ELISA. We offer a large catalog of other ELISA types on our webpage. We don't have video content for them yet.
Why can't we pippette out the liquid? Why to we have to dab it onto tissue paper?
Pipette washing is a common practice and a widely acceptabled way to remove liquid. Many labs will use a clean pipette tip hooked up to a vacuum to suction out liquid. When doing so, please tilt the plate at an angle and be careful not to scrape the bottom of the well with the pipette tip.
It's best if an experienced team member demonstrates this technique prior to a new user attempting it for the first time. If this is not possible, then it is best to blot on tissue paper to ensure all liquid is removed. The goal is to remove all liquid while maintaining the integrity of each well during the removal step.
Mam you are very beautiful 😍
Nnsr
𝚙𝚛𝚘𝚖𝚘𝚜𝚖 🏃
Not easy
What kind of additional information or demonstration do you think would make it easier?