Finding The Best Tissue Culture Media For Philodendrons - Part 1

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  • Опубликовано: 15 ноя 2024

Комментарии • 25

  • @emilylovesplants
    @emilylovesplants 2 года назад +4

    Would love an update on how these turned out! Also- what part of these philodendrons would you recommend using for TC? These look like they are plantlets - would you recommend doing a node if sterilizing and trying this from scratch?

  • @heehawcowboy5157
    @heehawcowboy5157 2 года назад +2

    What kind of philodendrons are you going to try the rare one Pink Princess Philodendron? White wizard? arent all the plants species the same since they are in the same family or does the sub species actually make a difference?

    • @PlantCellTechnology
      @PlantCellTechnology  2 года назад +1

      Different species of plants are needed to be considered while working with the plants in tissue culture. So, it's highly recommended to tweak bit your media recipe whenever you are working with the new plant.

  • @RusticRaver
    @RusticRaver Год назад

    So the galangal, is it simply powder food grade from plant like you would use in thai soup? Or is it special filtered for plant tissue culture only? thx

  • @makerspersona5456
    @makerspersona5456 2 года назад +2

    Isn’t TDZ dissolved in DMSO normally?

    • @PlantCellTechnology
      @PlantCellTechnology  2 года назад +4

      You can also use 1N NaOH. DMSO can cause a toxic effect on plant tissues.
      You can read the reviews of other researchers about the same here: www.researchgate.net/post/How_can_I_dissolve_thidiazuron#:~:text=I%20have%20used%201%20N,of%2010%20mg%20%2F100ml%20stock.&text=Yes%2C%20you%20can%20use%201N%20NaOH.

    • @makerspersona5456
      @makerspersona5456 2 года назад +1

      @@PlantCellTechnology thanks so much for helping me to avoid that. You just add it before autoclave? I’ve read that there’s some controversy about whether it loses its effects?

    • @PlantCellTechnology
      @PlantCellTechnology  2 года назад +1

      @@makerspersona5456 Yes, ideally PGRs should be added after autoclaving the media, through filter sterilization because autoclave heat can damage them. However, for the TDZ case, different scientists have been trying different ways, either before or after autoclaving. Often, the damage to hormones is made up of the media nutrients. But, again it's on you, considering the damage, how precise do you want to be with the hormone concentration.

  • @sheilaelrod6115
    @sheilaelrod6115 2 года назад

    Has there been any update on progress of this experiment?

  • @makerspersona5456
    @makerspersona5456 2 года назад +1

    Isnt 6BA normally dissolved in 1M hydrochloric acid? What’s the advantage of using sodium hydroxide?

    • @PlantCellTechnology
      @PlantCellTechnology  2 года назад +2

      You can either use 0.1 M HCL or 1N NaOH to dissolve 6BA.

    • @makerspersona5456
      @makerspersona5456 2 года назад +1

      @@PlantCellTechnology Do you think KOH will work too?

    • @PlantCellTechnology
      @PlantCellTechnology  2 года назад +1

      @@makerspersona5456 Yes, KOH will also work.

    • @merlinaroyeras4581
      @merlinaroyeras4581 2 года назад +2

      95% ethanol minute amount can dissolve BA or IBA too.

    • @PlantCellTechnology
      @PlantCellTechnology  2 года назад +1

      @@merlinaroyeras4581 Yes you can use it as well to dissolve these hormones.

  • @Aroid_Hunter
    @Aroid_Hunter Год назад

    And update?

  • @ashaakshetty5313
    @ashaakshetty5313 2 года назад

    Any updates about this experiment kindly inform

    • @PlantCellTechnology
      @PlantCellTechnology  2 года назад +1

      Currently no such updates. We will let you know as soon as the update is available. Thanks for showing interest in our videos.

  • @kokleongong5560
    @kokleongong5560 2 года назад +1

    I was enticed by the various videos published by you and decided to make purchases of your product end Aug 2022. However, your company made a terrible mistake by failing to declare the shipping cost. As a result, a higher goods and service tax was imposed on me by the authorities. I have since written two emails to your company and no one is responding. I have to fork out this additional tax to get the products delivered. To my surprise, your company sent me an NAA expiring this month (Sept 2022) and MS media (50L) expiring Oct 2022. Is this how your company conduct your business?

    • @PlantCellTechnology
      @PlantCellTechnology  2 года назад +1

      Hey Kok,
      Sorry for the inconvenience caused.
      I have forwarded your query to the concerned team and they have reached out to you.
      You can convey all your concerns, ranging from shipment and product issues to the team. They will try and do their best to resolve them at the earliest.
      And, if there are any concerns left, you can reach out to us at info@plantcelltechnology.com or anjali@plantcelltechnology.com.