Tissue Culture Q&A
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- Опубликовано: 30 сен 2024
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Timestamps:
1:05 TC Timeline
3:53 Begonia tissue culture
8:03 Q&A
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Whats your disposal protocol for media/disposable containers?
Bro, this is what YT was made for. Love the channel
As a YT lady. I approve of this comment
The part about you being able to do all this even if you studied finance really set in my mind that it's still not too late for me to be scientifically-proficient even though I didn't end up choosing a science major. I've always wanted to be a scientist but different factors led me to take a different route, and it hurts a bit to not be able to fulfill my childhood dream career. Anyways, I've only very recently discovered your videos and I'm binging all of them, and I really commend the comedic gen z humor your videos have while also being comprehensive yet succinct without being too intimidating for people who are new to this (like me)
Also, I might just be one search bar away from finding this all out, but I was wondering if you could make a video explaining why exactly the recipes for plants are different? If there's a pattern to it that we could take into consideration in case a plant doesn't have a recipe, and how we can adjust the ingredients to yield better results. Like why the recipes for a verrucosum and serpens are different, what they have in common, and how we could tweak the recipe for a plant that doesn't have one like the verrucosum x serpens (just using random names as an example)
Do you want to become Poison Ivy? Because this is how you become Poison Ivy. Use your powers for good. 😁
Looking forward to the in-depth videos on organogenesis and somatic embryogenesis!
Theyre already available on her onlyfans lol 🤣
Underrated comment 😂😂😂
Regarding C. sativa, you can always do a demonstration with industrial hemp. It grows like a weed (pun intended) here in Texas. It sticks out once you learn to recognize it.
PLEASE call it "Audrey II" for a few reasons.
1. Monster plant.
2. This is your second try.
3. You get to say "Feed me Seymour" in a fun voice 😂
This is a reference to 'little shop of horrors' for any who don't get it. It's a play about a Monster plant
I'm joining the tissue culture journey! I ordered everything and more off of your starting list. 🎉
Keep me updated on how it goes!
Keep US updated ❤
i love your videos, and you have been my main motivation to open a small business using tissue culture
That is so awesome. Thank you!
Bringing down Big Plant ....
The empire is expanding...
I'm an American plant junkie that's been living here in Thailand......If there are any plants you want from here...just let me know. I've been dying to learn tissue culture forever....so I'm your fan-boy! A very old fan-boy.
Please helllp! 😭 I messed up!
I accidentally added 30gr/L of agar instead of 6gr/L. I used an expensive DKW media and other expensive additives and hormones and I don't wanna waste it!
Will this too high concentration of agar affects plant growth?
plants in jailcells 😭😭😭😭
This was the comment that took me out 😂😂😂
Your videos have popped up a few times on my feed and I have enjoyed them. Brings back memories as I have a degree in Mycology (mushrooms) and a lot of what you do, I've done in the past. For those suggesting you do mushrooms, I say go for it. You can do tissue culture from most parts of the mushroom, just make sure you sample from an interior area protected from any contaminants. Standard medium is PDA (Potato Dextrose Agar) The recipe is available on lots of sites.
Personally, I would like to see you do it because it will highlight where you need to improve in your sterile work under the flow hood. PDA will grow all fungus, molds, and yeast so one small mistake will lead to a contamination of the plate. It took me 100's of plates to get my contamination down to below 2%. ;)
Hello @GrandpasPlace ! I am a frustrated marine mycologist, I say frustrated because my field is actually fish physiology. I wanted to shift to marine mycology but the institution I worked with did not allow me to. Nice to see a veteran mycologist here.
You can try to grow a fluorescent mushroom it's very pretty
Great work! Thanks to you I I have been grow strawberry in tissue culture for a month now. I have only 15 jar, but they grow excellent!
Neat! First video of yours I've watched. I'd love to try it but my hands shake too much.
About schools: in 2014 I sold my business and went back to school. Now up to 5 degrees. Next is Ms in Biology and BS in Hort. You can do it too. Go for it. If you have questions, ask.
did you just say you don't like mushrooms ???
(wow)
I said what I said! lol
@@plantsinjars in public even ;)
Its so nice to find another person talking about begonias and african violets that isn't 3x older than me. Love the content!
AYO Fellow Begonia lover! heck yeah!
Typing in "Oh I hit my finger so hard".....Hmmmm, not the results I was expecting LOL
Stumbled upon your channel, love the videos, not I'm gonna end up spending money for a home lab i didn't know I needed, but now really really want 😂
I started tissue culture 2 years ago and I did tens of attempts and everything was a complete failure until today :(
But I never give up and I'm still striving to see a sprouted explant!
The interesting fact is that I don't have any fungal contamination (0% fungal) and the only thing that's ruining everything is endogenous and exogenous bacterial contamination from my explant! The last time I almost killed most of my explants in the sterilisation process but again I got contamination from the explants 😭
Try growing your plants that you cut off of sterile , grow with hydrogen or oxide, spray the plant with wet table sulphur to clean it with , before you take your cuts spray the plants down well with hydrogen peroxide/ water mix , 35 percent hydrogen peroxide at 10 mls a gallon. Then cut your material from that plant. This will help a lot getting your tissue through clean.
I absolutely love that you’ve created a whole business out of this. I definitely assumed you had a biology degree of sorts, so double kudos to you for learning all of this yourself. I will say, I’m quite sad that you’ve switched to disposable containers. I’m not sure what the benefit is since you still have to sterilize them. And it’s just so much unnecessary plastic waste 😞. Nonetheless, love your videos and can’t wait to see the next one!
There’s allot of benifit in using plastic including not having to expose yourself to contamination to clean you just throw them out, at a exponential size it can be very expensive to invest in the same amount of mason jars and lids etc
I thought for sure you had a lab tech background. We never know what our passions might be.
We need you in the cannabis industry ❤ thanks for the nice content
Call it the Frankencutlure or saladculture jar, I'm leaning towards Salad culture🤪
Tissue Terrarium Rex
Fascinating, digestible and informative. A subject I have never been exposed to, but I am very much enjoying your tutorials. Also, love your philosophy.
I found your content extremely informative. I look forward to your future content. I've only had a chance to view 2 of your videos. I looked at your etsy store and would be happy to shop there for rare tropicals, sorry not a begonia fan. I am assuming the Thia Constellation was tissue cultured and hope to find that in one of your videos. You had at least a 1/2 dozen including some Hoya, Burle Marx I am still in the market for. I have been successful with cuttings. Like I said I can't wait for you to finish your series. I subscribe to a few channels and would love to see weekly content. Too bad TC takes so long I came across an affordable batch of Spritus Sancti, but with my luck, the price will have dropped considerably before the plants are viable and I don't have the luxury of your weather living in MI. TC works for me with rarer plants because you can buy anything of real size here. A 4ft monstera is nearly $100 and if you try to ship larger plants they just get damaged. I put almost all my large plants on the balcony for the summer and they grow so well I don't care how much you spend on lights nothing beats mother nature. Come fall everything has to be treated as organically as possible for pests repeatedly I lost half my collection in 2 weeks at the start not keeping a close enough eye on thrips and spider mites. Now I know which big box stores sell plants that really attract them so I go buy some sacrificial ones that I really keep an eye on and place them just outside the main growing areas which has worked well and the plants can handle extra pest treatments better than some of the harder to grow plants. I am amazed that you didn't study botany in college. I do agree with you having shopped Thailand I came across a number of sites that had much cheaper shipping for tissue culture for rare tropicals. The one site would have shipped full-size mother plants for what you were charged. I guess I will have to sign up with a few social media platforms rather than just here if I am going to be able to follow you. I appreciate all the extra information you include like the names of a few places in the states doing tissue culture, where you can go to see it done. What better reason than to plan a trip to Hawaii.
Hello from Chile. I bring grow regulators from my stay in Europe, but they came in powder presentation. what is the best way to handle them or prepare stock solutions to work with?
I love your channel and I am very excited to start in TC ^^
Shout out to a fellow Madd Scientist!!!
2nd “D” is like the Addams family.
It stands for “Different”
For Kryo temperatures - USE LIQUID NITROGEN
I cringed when you hit your finger... I've only recently found your channel so the trauma is still very real here. Can I ask to what extent you need to be careful with waste containing the PGRs? The used media and waste water from washing the plants or washing containers, etc. How do you dispose of your troubled waste? Can't wait to watch you on this incredible journey.
Many thanks from Sydney, Australia.
How does a court change a law/policy? Politics is for policy change. That that is what 4 pillars of democracy means.
Hi question: can you grow micro cultures from other seeds? You mentioned growing orchids that way and I'm only able to find rubus chamaemorus seeds even though I've been looking for a plant/cutting/bareroot for over a year
Dicing through your tendon must have hurt like all hell. I've done stuff like that when I was younger and always get squeamish when I think back on doing it
Have you figured out a name for your Jar O Cultures/ And when you say " Don't quote me, I am not a professional...' I believe that the second that you get paid for doing something, That would be considered Professional. No one would want to pay someone for doing something if they didn't know what they were doing.
Commercial propogators struggle to make a proffit. My question is why do it at home other than fun?
Hi Laur. You mention that people from discord helped you for one of your begonia protocol. Are there any discord server dedicated to PTC enthusiasts that you know?
Hie my sister I really happy to see your practical nice works, God bless you
Hi, I’m new to this. And you mentioned somewhere at the end of Part II, that on that big glass beaker or jar that the plants are contaminated? What do you mean by contaminated? And how do you know it is contaminated? How do I find out that my specimen is contaminated and with what? I’m just learning tissue culture from you. Thanks
I couldn't find in your videos what kind of paper you use to make the micropropagation in the sterile cabinet. do you use normal kitchen paper or another kind? do you sterilize the paper in the autoclave? how is it done? thank you very much for your videos and your work!
Can you keep them in there in the jars forever? And when they grow to big split them up? Or do they “mature” and need to be transplanted eventually
I mean, jungle juice is the obvious name for the plants you throw in the Erlenmeyer flask
Love your videos! Super interesting! :)
I just came across this video in my recommended, and when you read the question at 10:20, I was literally thinking about the Lyon Arboretum at UH Mānoa. They're doing really important work to keep the plants my ancestors once used!
It's just a shame we can't do the same for our endemic fauna--at least not in the same way.
Dont cut the plastic tubs. Buy a drill and holesaw otherwise your going to risk another injury
Cut my flexor tendon in my thumb doing something so very stupid and yea you actually need your thumbs ! 😅 had the same crazy looking splint and physical therapy so it happens !
If you have a flask that gets contaminated could you treat it with hydrogen peroxide?
I’m guna need you to upload way more often! Please. I need to learn all of this and I’m a binge watcher type lol I need all the videos 2maro
As soon as you mentioned mycorrhizal fungi networks I was like "oh yeah nice we'd get along great!", then you said you don't like mushrooms.
I'm not sure what to tell you now.
Jokes aside, I love this channel. I've learned so much. I've been propagating aquatic plants using various hydroponic systems, but I've been struggling with propagating enough of certain plants. I'm realizing this might be the answer to mass-production of babies to grow out, even if the initial time input might be significant. The scalability seems incredible! I'm also familiar with mycelium culturing and have the equipment and procedures figured out, so hopefully it transfers over well. Thanks for putting this information out there! I hope your finger's healed, I just watched that scalpel accident video.
One thing I never see mentioned is air during multiplication and rooting. Do containers need air hole and filter patch, and if so, what size hole. Only hole I have seen is on biocoupler.
Imagine you can achieve success with something rare like Encephalartos latifrons going at around +/- $1000 per inch 😦 I must definitely try this out.
So I’ve heard verigated stuff is very hard to make prolific in each plant ?? I was gonna ask how successful you are at verigated plants? I see your Etsy has a lot of verigated items. Is the company I got this info from doing something wrong? Secondly, have you ever tried verigated bananas? Thx
you do NOT look like a mad scientist at all. I've met enough of those, you don't. really.
Your subscriptions would go through the roof if you would cover the funny plant
There is only one name option for the flask: Frankenstein’s Lab.
Is there any reason why you use two explants? Do you sell or sources where I can buy the tools required in plant culture? Can I store explants for sometime before use?
great lesson , thank you :) your every video inspiring me towards tissue culture
Another amazing video, chock full of information. Every minute is educational, entertaining, or both!
The jar should be called Frankenstein
Сколько % отбеливатель продается в магазине в США ?
🔥🔥🔥🔥🤘😛
How about calling it the "FrankenPlant" flask? 😊
Can you sterilize with an instant pot? I assume so just don't know how long to keep them in.
Your mom sounds lit. I also recommend everyone eat 1 mushroom a day.
Nice to see you're focusing on b2b orders now. How's it going for you?
missed "plants in bars" joke opportunity 😭
Hello Laur. Yes... I know you think I need a crash course in pop culture because I am on my forties and I don't know who is Hanna Montana. I'm absolutely loving that program from the University of Hawaii you told about. Preserving all plant species is so important, that every country should definitely hop on board before it's too late. Thanks for sharing your knowledge with me, Laur! I'm glad I get to learn all these amazing things with you! Some beautiful plants and flowers for you🏵🪴🌵☘🌺🌷😉😉
You mentioned a discussion on discord, can you share the discord group?
Hey, can you please share the model and make of your Flowhood?
Name the giant flask “Big Birtha”!! 😂
There's some cool mushrooms out there some even glow in the dark
Did you ever get the synthetic seeds to germinate?
Can you clone 100 new fingers from 1?
is a bunch of cannabis cells legally a cannabis plant??🤔🍀
how do you tell the jar has been contaminated?
So dig this channel thank you.
What size are those deli containers you use??
Why don't you ship your products to Turkey?
You have the gift of teaching :)
I see a video by Hiesenplant and I click.
Nice work
Laur i love you 🥰🥰🥰🥰
mycorrhizae are super interesting
hi, Sweet Laura how do you do.....???
I think you can reach that goal, rooting for you 🎉
Ever tried aloë for propagation?
the naruto refrence was wild lol
How often do you get Contamination? It's funny in mycology Trich is contamination for mushroom cultivation but in the plant world it's used in mycorrhizal relationships. Any thoughts?
Usually around 10% or so get contaminated. Trying to get that number as low as possible though
Can I get an invite to Discotd server?
Is it possible to tissue culture a Hoya?
Which light timer do you use?
Call it Scoby Dopie Don't
Don't care bout anyone else but you
Name her Monstera Erlenmeyer
Your instagram link in the description isn't working. Just a heads up. Thanks for the videos
thank you! think i fixed it
PLEEK NOT PLANTS IN JAILCELLS💀
Try glowing mushrooms
Frankensoup 🌱
Call it "THE MOSHPIT" ☺
HI. Laur
l Love you 😍😍😍