Cannabis Tissue Culture and Germplasm Storage - Mark Jordan
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- Опубликовано: 11 июл 2024
- Tags: Tissue Culture, Propagation, Cultivation
Mark Jordan presents "Cannabis Tissue Culture and Germplasm Storage" at CannMed 2019.
After starting off in 1902, Cannabis tissue culture is quickly becoming a mainstream form of commercial Cannabis propagation. There are several hurdles that propagators need to overcome in order to successfully perform tissue culture on Cannabis. Once in culture, the possibilities are endless. Addressing these issues and understanding the applications that tissue culture presents are key to the advancement of the industry as a whole. Mark has developed protocols over the years and plans to shed some light on the problems and solutions of the cannabis tissue culture industry, along with addressing some of the applications such as germplasm storage along with data surrounding the costs and benefits.
0:00 Introduction
0:50 Why use tissue culture on Cannabis?
3:25 How do you know what chemical concentrations to add?
4:43 Understanding plant morphology
5:39 The 4 Common Stages of Plant Tissue Culture
6:53 Initiation
7:37 Meristem Culture For Virus Elimination
8:36 Stage 1 - Axillary Node Culture
9:47 Multiplication
11:55 Future Shipping
14:22 Acclimation - Herdening
15:23 The 4 main problems with Cannabis TC
16:24 1.2 Better Cytokinin Auxin combinations
17:01 Endophytes
17:53 2.1 More Pathogen Pictures
18:10 2.2 Pathogen Elimination Explant Prep
18:48 Common Stage 3 - Rooting Problems
20:56 Common Stage 4 - Acclimation Problems
21:39 Germplasm Conservation
21:57 Germplasm Storage - Refrigeration and Freezing of Cultures
23:34 Plant Breeding 
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Wow. Excellent presentation!
Amazing presentation. I learned a lot here and cant wait to progress in tissue culturing.
Dude, great presentation! You know your stuff and you delivered the info in a very understandable way. Fuzzys 4 Life! =)
Excellent info, thank you for sharing
Love this presentation!
Great content, thank you very much for share it
Excellent Information/no BS
very good presentation
Fantastic presentation. Went straight in and didn't talk down to his audience.
Thank you.
Truly informative
Good to go commercial
The future
well informed :D
This Dude’s Awesome!
knows his stuff!and I’m related to him 👍🏼
Great Job Conner 👏🏼
Question for Mark: Concerning Endophytes in large growing plants, when growing out large plants in the garden is it possible to treat them with H2O2 at the levels you concentrations by water watering and spraying? Also, for the general population easily obtaining concentrated H2O2 is difficult for most as pharmacy grade concentrations tend to be 3% by volume. The levels you use tend to be 5-12%. Will 3% be strong enough and if so, how should the general gardener apply it? Taken literally, based on your information, I would assume if feeding a 5% concentration with nutrients that the H2O2 5% concentration is used to then make the nutrient feeding solution, and not that a separate application of H2O2 solution is used as a drench after, or before, feeding. Is that correct?
I'll try to answer this fully. Let me know if you have any other questions and I'm happy to help. I don't get notifications to comments on this video so feel free to message me directly or find me here www.linkedin.com/in/markgjordan/.
I tend to water with H2O2 because it prevented anaerobic bacteria from building up due to the oxygenated soil environment. In turn, that started the elimination of some endophytic bacteria. You can spray it (which shouldn't effect endophytes) but I found the initial step to get a plant into stage 1 by using bleach and tween 20 more effective for the removal of pathogens that a spray would take care of.
3% can definitely be strong enough, a lot of it just depends on the pathogen.
As a gardener with access to only 3%, I would take that and mix my nutrients into the 3% (if you have lots of things in your nutrients that react to H2O2 such as high iron I recommend starting without them to maximize your oxygen concentrations instead of making something like rust Fe2O3•32H2O).
To apply it, just water to the point where you have lots of runoff. For example, when you have a pot with 1 gallon of soil make sure to use at least 1-2 gallons of water containing the dilution of H2O2. I would do this daily for 2-3 days then let the plant dry out over the next 2-3 days. If the plant isn't drying out in 2-3 days you may need to adjust some variables such as pot size, soil drainage, light intensity, or room humidity.
On a side note, to save money it may be easier to root a cutting and use the H2O2 water on the fresh cutting until it pushes roots and then another week or two after you see roots. The H2O2 shouldn't hurt the cutting or rooting process if it is 3-5%.
@@Komatchi Can I use Hypochlorous Acid (I have a generator to make) and at what level ? I use this in my RDWC with an ORP meter , works great
I have been doing tissue culture for about two years now , I have learned a lot but am still a rookie.
I have been using 35 percent hydrogen peroxide at 10 ML a gallon. I’m not sure what percentages it mixes at with water. I do have pretty good luck getting the plant material clean to get it through the culture. Because of the prevalence of HLV I have also had to incorporate testing into my tissue culture process.
Many times HLV will not clean out of the plant material. There has been some success with meristem culture to clean HLV from the plant.
I have had almost no success just cutting tissue out of a field or garden and trying to culture it right away. The plant always has to be washed and cleaned and grown clean to get through the culture.
I use sulphur to clean with and spray the plant down with it frequently. I would like to hear your opinions on HLV. Also any suggestions on pre mixed/designed protocols to use for various different types of cannabis plants. The one I am using seems to be good for most plants but not all. Kush strains kill me but I do not have the time or resources to develop protocols for each strain. Thanks in advance. I enjoyed the video very much and my experience agrees with everything you are saying.
Nice present
30:08
Is it just me wanting more content from him or a is that Vader OG rust?
Thanks for the very interesting video.
I would be personaly very interested to know more about the potential of in vitro culture to help germinate difficult seeds (old seeds for example), as a genetic pool preservation measure for endangered or semi-lost cannabis species ( such as lost landraces).
Any thoughts on this particular topic?
Totally! To understand this process understanding what is a seed is is crucial. A seed is basically an embryo wrapped in a hard shell of everything it needs to grow in terms of nutrients.
(a little background here) Seeds only germinate when an enzyme activates (typically water activated).
When discovering macro and micronutrients in plants, researchers took seeds and removed the seed coat. This was because the seed coat contained 1 part per billion (not 100% sure on that number, a very small amount anyway) or less of certain things. By removing the seed coat it removed the little amount of nutrient it provided. Over time, by depriving the media of that specific nutrient, the scientist could find what nutrients were critical to plants. This is why silica is known to be useful but not needed in fertilizer.
Back to the point; By removing the seed coat, you essentially remove everything the plant needs to grow. You also eliminate defective enzymes that inhibit germination. As long as the embryo is alive (meaning the seed hasn't cracked and dried out), the tissue culture media will provide everything the embryo needs to grow in theory. It requires cutting open the seed coat and germinating the embryo without it.
@@Komatchi a-man
How do you involve yourself professionally if some one decides to go commercial
What would be investment in Lab land to grow one million plantlets a month and ROI
What does he mean there are no antibiotics? Even the most basic media protocol has PPM (Plant Preservative Mixture) in it.
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Nice
Does anyone know if meristem tissuculture is needed to cure root rot (since some people say it's systemic) or can it be done through auxillary node culture? Or even normal cloning? Thanks
Been trying to heal my plants taking cutting and can't seem to be able to cure them. I keep getting the infection come back...
@@jhallak94 probably the mold or fungi is within your plant... pendong on wich country youre in, yhere are products (usually in powder) that kills the fungi, if you really want to preserve this plant, treat it with a antigungicidal powder then do a tc
Cannabis cannabis 🙌 woo woo 🎵🎶🎵🎶
Would TC work on a polypoid.
Yeah it can, it's actually how some polyploids are made.
Does he sell tissue culture kits
Nope I don't.
Phytotech sells cannabis tc kits
how would I find the test strip for viruses
Just look for the ImmunoStrip® tests
@@Komatchi does immunistrip test for hop latent viroid?
who is this guy and whats his company?
btw google some shit and his name is Mark Jordan Chief Executive Officer at Minnibis
@Peter Lustig You obviously have no idea what you're talking about.
"axillary" is not pronounced like "auxiliary", but rather "axe-ill-airy".
a 20 years old clone get old? i thought it was forever
Think of it in terms of a human. When you were a kid your DNA was fresh, you had no stress in your life. Nothing to beat you back. Over time, your genes start to change slightly.
Plants do it in slightly different ways. 20 years of being cut over and over along with other environmental stresses such as drought stress will change how the plant yields and performs.
Y
chemotype or chemovar man!
P
apecall meristems WTF yess spelled wrong no doubt, can anyone translate this videdo to english,,,, shit i should have stayed in school
when he was in school did he learn biochemisrty or something. my school never taught us anything at all to do with crazy sience stuff like this. we blew up a couple tesr tunes with fire and stuff, maby burned a magnesium strip and oooowwooaaaaaa'd at it but no actual real stuff. WHAT COURSE IN A COLLAGE WOULD YOU TAKE TO UNDERSTAND OR START TO LEARN ABOUT THIS STUFF????? he certainly didnt start cloning plant tissue straight after P,E
can someone please tell me what course and what area of education. is it biology chemestry ???????
@@adamlol3955cell or genetic biology or botany may put you in the field you’re looking for, but it will be two years of general ed in bio and chem before you actually have some of the pre reqs to do this work, but normally then you need an upper division bio course, then you might be able take the course. I was on a different pathway, but mammalian cell culture was available to take if it had fit in my schedule. We didn’t have a plant cell culture program in undergrad, but I know of some professors who may have been open to it in the masters program to study plant genetics.
Honestly with all that said, you really don’t need to understand much of the science unless you’re doing a lot of troubleshooting. There will a procedure explaining the equipment, and materials needed. Following that as closely as possible under the suggested condition should get you pretty far in any line of science. The biggest risks are losing your samples and wasting some more through some trial and error. Second is sometimes you may develop something unintended, such like mixing two ingredients you’re not supposed to like bleach and vinegar, or grow pathogens like mold or bacteria. In that case I recommend being willing to toss out any dangerous or suspicious samples, and again, follow the procedure closely, and verify using a safety data sheet (SDS) what is in the product you’re using and any risks involved.
To answer your question, plants have nodes and meristems. These are areas of branching and growth. Apical meristem is the primary area of new growth.