Sir plz tell me how to make dilution of urine For urine creatinine test 1:51 ratio is given in literature....1 for Urine and 51 for distilled water..now how much volume of urine I take for making dilution 10 ul, 50 ul, 100 ul or what???
No sir. Blanking should be done with reagent only as it is clearly mentioned in literature. However, if some other manufacturer mentions distilled water for blanking, you may have to follow that procedure. Generally in creatinine all kits have coloured working solution. Hence, we need to reduce this absorbance from the final absorbance so that we only get the absorbance from reaction of working solution with creatinine in sample. If we do not do it, then the absorbance of working solution will interfere with the absorbance of reaction of working solution with creatinine and it may give false high result.
Dear Madam, Creatinine ka working solution jab hum banate hai to uski shelf life bahut he kam hoti hai - karib 48 hours agar hum usse 2-8 degree celsius pe store karenge to, jiski information hume us kit ke pack insert me bhi mil jaygi. Isliye jab hum working solution morning me bana ke room temperature pe rakhte hai to uski stability compromise ho jati hai jiske wajah se results galat ho jate hai. Isliye hume afternoon me ya evening me fresh working solution banana padta hai. Isliye jab hum fresh working solution banate hai to dobara Reagent Blank aur Standard run karna padta hai aur fresh factor ko establish karna padta hai. To, creatinine me din me 2/3 baar Reagent Blank aur Standard run karna padta hai. Anamol ke kit me working reagent ki stability 6 months ki hoti hai agar hum usse 2-8 degree celsius pe rakhenge to isliye hamare kit me apko din me 2/3 baar Reagent Blank aur Standard run karne ki zarurat nahi hai.
Yes you can use. Kindly make sure to reduce the sample volume by half also to maintain reagent to sample ratio. In that case, you can set aspiration volume to 350 microlitre.
Thank you madam. You can use any standard semi automated biochemistry analyzer available in the market and it can be procured either by contacting the company directly or their authorized dealer.
No sir. Blanking should be done with reagent only as it is clearly mentioned in literature. However, if some other manufacturer mentions distilled water for blanking, you may have to follow that procedure. Generally in creatinine all kits have coloured working solution. Hence, we need to reduce this absorbance from the final absorbance so that we only get the absorbance from reaction of working solution with creatinine in sample. If we do not do it, then the absorbance of working solution will interfere with the absorbance of reaction of working solution with creatinine and it may give false high result.
Sir, the method is rate kinetic or fixed-time kinetic. If you wish to know more about the method and how it works, you can check our another video in which we have explained how end point, kinetic and fixed-time kinetic reaction works. We have shared the link to that video below: ruclips.net/video/niO5U0AXOw0/видео.html The principle of this kit has been explained in the literature which can be downloaded from our website. We have shared the link for the same: anamollabs.com/creatinine.html Please help us to know if we have answered your request.
Sir, SGPT me kit me factor diya jata hai. Apko standard run karne ki zarurat nahi hai. Lekin agar aap multical run karna chahte hai to exactly sample ka he procedure follow karna hota hai. Aap hamare video ko dekh sakte hai jisme humne SGPT ka procedure explain kiya hai. ruclips.net/video/dbDMgKyTeNM/видео.html
Dear sir, The logic of performing the test will remain the same as shown in this video. However, if you wish to discuss exact details for a specific brand, we request you to get in touch with our subject matter expert via our website - www.anamollabs.com
Sir, humne dusre video me explain kiya hai standard ko kaisa run karna hai. Apse request hai us video ko dekh lein: ruclips.net/video/wTdioT76cq8/видео.html
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Nice explanation but I have a question what are the volume used for the standard and working solution in the test tube
Thank you this video was very useful 💜✨
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Why does different laboratories have different results?
Good information 👍. And useful for knowledge.
Glad you liked it
sir working regent बनाने के बाद तुरंत हम रीडिंग ले लेते हैं कि कुछ समय के लिए wait करते हैं
Bhai.. ye j Factor wagera hita h.. us ko kesy banae Machine py
ek videi Uplo kri
Bhai ye hb biochemistry per kasa kary zara bataye vedio ma
Sir plz tell me how to make dilution of urine For urine creatinine test 1:51 ratio is given in literature....1 for Urine and 51 for distilled water..now how much volume of urine I take for making dilution 10 ul, 50 ul, 100 ul or what???
Thanks for the good explanation 😊
Thank you very much sir. Please do subscribe to our channel and share this video with your friends and colleagues!
Why we are waiting for 10 secs ? I can't understand that..
Thank you sir. I want to more stady this test this method.
How to run lypemic sample plz make video
Kya test ko diluent kar ke test kar sakte hai agar high ho to
we need to know how to use the calculations manually
Any difference between jeffos method and modified jeffos method,my mothers creatinine shows low level when the same lab uses modified method......
Sir agr saare test Ka agar Hindi me bataye to Jada a chhe se samjh me aata
Yes sir hum saare videos hindi me bhi upload karenge
What's the name of the machine and how much
water bath ma nhi rkhna tha???
No sir
What is five hundred microliters?
Excellent
THANK YOU VERY MUCH 🙏🙏🙏
1000miclit working solution add 50miclit sample wix and aspitate the sample read value
Yes sir. Please follow the procedure as mentioned in this video for any manufacturer kit.
Instead of blank reagent can we use distilled water
No sir. Blanking should be done with reagent only as it is clearly mentioned in literature. However, if some other manufacturer mentions distilled water for blanking, you may have to follow that procedure. Generally in creatinine all kits have coloured working solution. Hence, we need to reduce this absorbance from the final absorbance so that we only get the absorbance from reaction of working solution with creatinine in sample. If we do not do it, then the absorbance of working solution will interfere with the absorbance of reaction of working solution with creatinine and it may give false high result.
Din mein 2/3 creatinine test karne ke liye bar bar blank and standard karna sahiye kiya pehle? Ya ek bar karne se ho jata hai reagent khatam hone tak
Dear Madam,
Creatinine ka working solution jab hum banate hai to uski shelf life bahut he kam hoti hai - karib 48 hours agar hum usse 2-8 degree celsius pe store karenge to, jiski information hume us kit ke pack insert me bhi mil jaygi. Isliye jab hum working solution morning me bana ke room temperature pe rakhte hai to uski stability compromise ho jati hai jiske wajah se results galat ho jate hai. Isliye hume afternoon me ya evening me fresh working solution banana padta hai. Isliye jab hum fresh working solution banate hai to dobara Reagent Blank aur Standard run karna padta hai aur fresh factor ko establish karna padta hai.
To, creatinine me din me 2/3 baar Reagent Blank aur Standard run karna padta hai.
Anamol ke kit me working reagent ki stability 6 months ki hoti hai agar hum usse 2-8 degree celsius pe rakhenge to isliye hamare kit me apko din me 2/3 baar Reagent Blank aur Standard run karne ki zarurat nahi hai.
Hafte Me Ek Baar Or Mahine me 4 baar sabhi Test Kit Ke Standard Lgane Chahiye
Which instrument they used
sir cant we use 500 micolitre working reagent for analysis.if we set the aspiration volume of analyser 400
Yes you can use. Kindly make sure to reduce the sample volume by half also to maintain reagent to sample ratio. In that case, you can set aspiration volume to 350 microlitre.
Very helpful
Thank you very much sir. Please do subscribe to our channel and share this video with your friends and colleagues!
is we always use blank and standard before patient serum test????
Dear Sir, you do not need to run blank and standard for every sample. You can run them before running the first sample.
Sir creatinine factor Kitna hona chahiye company name- coral clinical system?
Madam, ap company ke application specialist se details le sakte he.
Really useful information.
Will you tell the name of apparatus with cost and how it can be procured.
Thanks.
Thank you madam. You can use any standard semi automated biochemistry analyzer available in the market and it can be procured either by contacting the company directly or their authorized dealer.
Sir in blank why you don't take 50 micritre of distilled water
No sir. Blanking should be done with reagent only as it is clearly mentioned in literature. However, if some other manufacturer mentions distilled water for blanking, you may have to follow that procedure. Generally in creatinine all kits have coloured working solution. Hence, we need to reduce this absorbance from the final absorbance so that we only get the absorbance from reaction of working solution with creatinine in sample. If we do not do it, then the absorbance of working solution will interfere with the absorbance of reaction of working solution with creatinine and it may give false high result.
Sir pdf ka link bi share kr do
Sir you can visit our website www.anamollabs.com vaha par mil jayga.
Sir what is factor in this ??
Sir, you are requested to introduce the method(reacting components of reagents and serum).
Please
Sir, the method is rate kinetic or fixed-time kinetic. If you wish to know more about the method and how it works, you can check our another video in which we have explained how end point, kinetic and fixed-time kinetic reaction works. We have shared the link to that video below:
ruclips.net/video/niO5U0AXOw0/видео.html
The principle of this kit has been explained in the literature which can be downloaded from our website. We have shared the link for the same:
anamollabs.com/creatinine.html
Please help us to know if we have answered your request.
Sir, Thank you for your request. We will make a separate video or the same.
Thank you very muchh
Thank you very much sir. Please do subscribe to our channel and share this video with your friends and colleagues!
Can you please add subtitles telugu please
Sir ji good
Sir SGPT ka standard kaise kiya jata h
Sir,
SGPT me kit me factor diya jata hai. Apko standard run karne ki zarurat nahi hai. Lekin agar aap multical run karna chahte hai to exactly sample ka he procedure follow karna hota hai. Aap hamare video ko dekh sakte hai jisme humne SGPT ka procedure explain kiya hai.
ruclips.net/video/dbDMgKyTeNM/видео.html
Nice video ☺️
Thanks 😊
Please show Coral kit method
Dear sir,
The logic of performing the test will remain the same as shown in this video. However, if you wish to discuss exact details for a specific brand, we request you to get in touch with our subject matter expert via our website - www.anamollabs.com
ئەرێ ئەوە بۆ نازانی وڵک بەشەر قسەبکەی کەربووم بەدەستەوە بژی بڤو نازانم چی یەک کەیلیمە حاڵی نابم خۆم جەومممم نیەەە
Thank you sir...
Thank you. Pls share this video with your friends and colleagues and subscribe to our channel.
Nice video
Thank you for watching video.
This Lab is ALT ALP
Nice
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Where is the creatinine level?? I look the whole video to see some creatinine measure... But there was no blood to measure at all
Sir ji glucose ka standar kaise lagate h 9335993597 hamara number h esme ap bata dijiye
Sir, humne dusre video me explain kiya hai standard ko kaisa run karna hai. Apse request hai us video ko dekh lein:
ruclips.net/video/wTdioT76cq8/видео.html
Nizzzzzz
What is the normal creatinine level
Thank you very much sir. Please do subscribe to our channel and share this video with your friends and colleagues!
Sir, each laboratory has to establish its own normal values. If not, you can refer to the literature of the manufacturer of the kit you are using.
Sir creatinine k standard ka factor kitna hona chahiye
Sir,
Aap jis company ka kit use kar rahe hai us company se aap factor application sheet maang sakte hai jisme apko apki information mil jaygi.
Tanks sir ❤
Hii
Fantrastic
Thanks!😀
Super expensive
Indian 🙄
Thank you so much sir
Nice