Estimation of Serum Creatinine By Jaffe's Method on Colorimeter || Biochemistry Practical
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- Опубликовано: 18 окт 2024
- Estimation of Serum Creatinine - This is the video on estimation of serum creatinine by jaffe's method along with the demonstration and discussion of viva question which will be helpful to MBBS as well as DMLT students.
Video contains -
Introduction to Creatinine - 01:09 minutes
Synthesis of Creatine and Creatinine - 02:03 minutes
Principle of Jaffe's Method - 05:25 minutes
Reagents for Jaffe's Method - 06:02 minutes
Preparation of Protein Free Filterate (PFF) - 06:35 minutes
Operational Procedure - 07:15 minutes
Limitations of Jaffe's Test - 09:00 minutes
Enzymatic Method for Creatinine - 10:39 minutes
Interpretations of Creatinine Value - 12:14 minutes
Creatinine Clearance - 14:21 minutes
Creatinine Coefficient - 15:46 minutes
Discussions of Viva Questions on Estimation of Serum Creatinine- 16:11 minutes
Demonstration on Estimation of Serum Creatinine By Jaffe's Method - 18:02 minutes
#Creatinine #renalfunctiontest #Creatinineestimation #jaffe'smethod #biochemistrybasicsbyDrAmit #biochemistrypractical #biochemistryvideos #dmltpractical
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that was awesome explanation. i personally got alot. thanks for making such nice video lessons
Thanks Hanif. Keep loving biochemistry and do spread the word about this channel. Tk Cr
@@Biochemistrybasics sir is there any another method. And which method gives accurate result??
@@rahimtamboli1044 Enzymatic method. It gives accurate results
@@Biochemistrybasics thanyou so much sir
@@rahimtamboli1044 most welcome dear
Best biochemistry lecture on youtube ❤❤
Glad to hear that
Sir , u r the best on whole tube
Please upload more and more videos of biochemistry for theory and practical of mbbs
Thanks a lot dear 😇👍🙏.
Thank you for this excellent video. As a point of clarification for the slide around time 16:55, creatinine is filtered by the glomerulus and also secreted by the renal tubules. This is why creatinine is not the gold standard for measuring/estimating kidney function. That would be inulin, which is filtered by the glomerulus, but neither secreted nor reabsorbed by the renal tubules.
Yes you are correct. But as inulin is not endogenous product creatinine is more preferred as compared to inulin
Love u sir❤. You are my practical teacher during this lockdown.
Thanks dear. Please spare a few minutes and do spread the word about the channel among your batchmates and friends
Incubation period is of 10 minutes.
TQ sir I'm a Tutor in Biochemistry dept nice presentation
Hello Mam. You are most welcome. Hope my videos are helping you in some or other way
@@Biochemistrybasics definitely sir as lm teaching for MBB s students sometimes I don't know how to start and what is to be told especially viva questions I started watching recently ur video s r very specific and helps me alot lm just Mbbs graduate
Dr Jyothi/ Jyothi lakka
@@jyothilakka9434 That's great. Almost 80% of syllabus is covered. You can find videos in respective playlist. Please do recommend youtube channel to your mbbs students too
@@Biochemistrybasics sure sir TQ so much
I am a subscriber of you sir.. please tell me what app you are used for making the video. (Especially for slide preparation)
I am using Microsoft power point only. There is inbuilt option of slide recording in it and dn I convert it into video
Sir please 🙏upload a series of lecture on Semi-automated biochemistry analyser.....both theory & practical.....so it can very very helpful for us......sir please consider my request as soon as possible 🙏❤️
Sure I will try my best 😊
@@Biochemistrybasics thanks a lot sir ❤️🙏
@@deepakcreation1497 please spread the word about the channel
very informative.. Thank you sir for making vd 🙏
Most welcome dear
Thank you so much sir for your valuable video.keep it up
🙏😇
Why is the vol of serum taken as 0.5 ml
Sir can you provide a private online class for biochemistry bmlt
Yes
Nice explanation sir! Tq.
Thanks dear
Sir sreum Creatine normal blood test aur jaffe method may kya difference hai plz explain????
Really very informative n useful...👌
Sir what will be the source of NADH and ATP during the enzymatic method..either its from in vivo or it will be added artificially..
It will be from the sample only.
We did this test but without using blank solution, only test and sample and they gave us homework on why we don’t use blank in some tests ?? Can you plz answer that 🙏🏻
Sar calculation mein blank samajh mein nahin aaya hai balance to jyada a raha hai to calculation kaise karenge blank to zero aana chahie
Thank you so much the video was very helpful
Most welcome dear
Thank you sir 🥰
Pls make video on estimation of urinary creatinine
Sure. Will do
Hello Sir, can you recommend me some literature about this old methods? It is very interesting, and this are very important basics. Today they use automates that do many thing behind the scenes
Sure will find out and let you know
www.google.com/url?sa=t&source=web&rct=j&url=gmcsurat.edu.in/lib/exe/fetch.php%3Fmedia%3Dbiochemistry:g-_group_various_method_of_glucose_estimation_gtt_and_carbohydrate_chemistry.pdf&ved=2ahUKEwi4452Ak5TyAhVk7HMBHck1B-cQFjAKegQIJhAC&usg=AOvVaw2Kd-WT975szcO-AgdAcTP3&cshid=1627969528659 Follow the link.
@@Biochemistrybasics Interesting, but i thought name of some old book that has many methods. This is great, but for glucose. What about, for example some hormones etc.?
Can you tell positive and negative interference [separately] that affect jaffes alkaline picrate method?
Dopamine, furosemide and bilirubin etc causes negative interferences
Cephalosporin, hemoglobin, glucose, lipemia and acetohexamide are positive interferences
@@Biochemistrybasics Thank u
@@jegatheeskriiynkaran4641 anytime
Sir, is normal secrtion of creatinine affect the GFR [increase/ Normal]?
And What happen to the GFR value if bacterial proliferation occur after urine collection?
Was very helpful....tq
Glad that you found it helpful. Please spare a few minutes and do spread the word about the channel among your batchmates and friends
sir one question, why u have written presence of protein in jaffe's limitation, aren't we using protein free filtrate????
Dts about presence of other protein in sample which interfere during estimation
@@Biochemistrybasics thnk u sir :)
@@shreyashjhunjhunwala3021 my pleasure dear. Please spare a few minutes and do spread the word about the channel among your batchmates and friends
What other laboratory method can be used to determine plasma creatinine concentration? Plz inform
Enzymatic method
@@Biochemistrybasics can you please tell me about the procedure and calculation of enzymatic method
@@sumaiyahaque1505 it will be written in kit insert
Sir pls say how to calculate Absorbance for blank, standard and test.
Take blank standard and test in three different test tubes....Set the filter at particular wavelength require for that particular test... Set the OD zero in the colorimeter with distill water and dn take the OD of blank standard and test.
Can you suggest an end point method for creatinine without deproteinisation?
You can use Enzymatic method but it's not an endpoint
what is the molar concentration of H2SO4? If we need to make PFF, what is the rmp of centrifuge and how long we should centrifuge?
1. H2SO4 - 2/3 M
2. In a centrifuge tube take 3 ml of DW, 1ml of serum 1 ml of sodium tugstate and 1 ml of H2SO4. Mix after each addition and centrifuge. Use the supernant as PFF. Speed should be optimum.
That's gold
Yes. Thanks
Sir a problem
When we make pff total amount of solution 4ml but in chart 5ml
How is this please help
My viva is tomorrow
Sorry can you please elaborate more
Sir, when you add 1ml DW + 1ml serum + 1ml sodium tungastate + 1ml H2so4 to make PFF, only 4ml is available but 5ml is used in the test chart. Please explain
@@indianvibes1329 It might contain one ml of working solution dts why. Please check your journal carefully. It might have written in it. In my setup we use ready to use reagents.
@@Biochemistrybasics thanks a lot sir
@@indianvibes1329 my pleasure. Keep loving biochemistry and do spread the word about the channel
U are the best 😭❤️
Thank you
What is the use of sod tungstate?
The storage form of creatine is creatine phosphate. True/False
It's stored in muscle as free creatine or phospo Creatine
@@Biochemistrybasics Ok Thanks
@@kritikavarshney7822 please spare a few minutes and do spread the word about the channel among your batchmates and friends
@@Biochemistrybasics oh I already have told them this. Thanks 😊
Can we prepare synthetic standard and serum of creatinine in lab.
Yes
Can you share with me
Sir,
What values should we get for test,blank and std normally
It depends on the concentration and method
why we use 2/3N h2so4 and 10%sodium tungstate in this method ?
To prepare protein free filtrate (PFF)
suggest me book for biochemistry practica
Read your biochemisry practical journal
How did u prepare a standard creatinine sol
Gooodddd great continueeeeee doctor how i ask some question personelly to sir doctor
Please
Superb
🙌😊
Thank u sir 😊
My pleasure shruti 😊
Sir what is the concentration of creatinine standard
It depends on the company
Thank you sir
😊🙏
@@Biochemistrybasics sir can u give carbohydrates and proteins same way with practical demo
Definitely. Some of the small videos like molish test, Benedict's test rothera's test hay sulfur powder test is already uploaded. You can check them in playlist (Practical)
@@Biochemistrybasics OK sir
@@Biochemistrybasics sir I got it (protein) but I also need carbohydrates and suger too
pls provide this pdf..pls sir
Please visit nextsus app
@@Biochemistrybasics sir i'm from Bangladesh.. i can't sign due to this reason.. pls provide this pdf link here
Sir what is the formula to calculate?
Dear it's mentioned in the video in the calculations
6:54
Hello sir
Hi dear