Haemoglobin Estimation- Sahli's Method (हीमोग्लोबिन टेस्ट) By Solution Pharmacy
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- Опубликовано: 10 апр 2017
- This video is also available in ENGLISH. Estimation of Haemoglobin by Sahli's Method (ENGLISH) By Solution Pharmacy • Estimation of Haemoglo...
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Haemoglobin is the major component of blood which carries oxygen to the tissue for various function. when that goes down below the normal level one feels lethargy and fatigue. it's always better to estimate the haemoglobin level in blood.
After watching this video you will be able to understand- what is haemoglobin test, haemoglobin experiment, haemoglobin in Hindi, Sahli's hemoglobinometer, how to check haemoglobin in blood, haemoglobin estimation test, find your haemoglobin count, haemoglobin level in man, haemoglobin level in the woman,
Haemoglobin can be estimated by two methods-
Sahli's Haemoglobinometer and haemoglobin colour scale.
Step by steps procedure to estimate haemoglobin in blood by Sahli's method.
1- Take out the Haemoglobinometer and arrange its component in a stable place.
2.Prepare 0.1N HCl
3.Fill the 0.1 N into Sahli Haemometer tube up to 2% mark
4. Now place the pipette in Sahli Haemometer at a given place (in between two windows)
5. Now sterilize the fingertip and prick it with the help of sterilized lancet and fill the blood into Sahli Haemometer pipette up to 20 microliter marks.
6.Now transfer that blood sample into Sahli Haemometer tube in which we have already taken 0.1 N HCl.
7.Wait for about 10 minutes to let the reaction occur.
8.Now slowly-slowly dropwise add distilled water to its side and shake well with the help of given glass rod.
9.Keep adding distilled water till the colour of blood match the side window
10. Observe and note down the reading, that’s the percentage of haemoglobin
हेमोग्लोबिन रक्त का प्रमुख घटक है जो विभिन्न कार्यों के लिए ऊतक को ऑक्सीजन लेता है। जब यह सामान्य स्तर से नीचे चला जाता है तो कोई सुस्त और थकान महसूस करता है। रक्त में हीमोग्लोबिन स्तर का अनुमान लगाने के लिए हमेशा बेहतर होता है।
इस वीडियो को देखने के बाद आप समझ पाएंगे- हेमोग्लोबिन परीक्षण, हेमोग्लोबिन प्रयोग, हिंदी में हीमोग्लोबिन, सहली के हीमोग्लोबिनोमीटर, रक्त में हीमोग्लोबिन की जांच कैसे करें, हीमोग्लोबिन अनुमान परीक्षण, अपने हीमोग्लोबिन गिनती, हेमोग्लोबिन स्तर में आदमी, हीमोग्लोबिन स्तर औरत,
हेमोग्लोबिन का अनुमान दो तरीकों से किया जा सकता है-
सहली के हीमोग्लोबिनोमीटर और हीमोग्लोबिन रंग पैमाने।
साहली की विधि द्वारा रक्त में हीमोग्लोबिन का अनुमान लगाने के लिए कदम प्रक्रियाओं के चरण।
1- हेमोग्लोबिनोमीटर निकालें और इसके घटक को स्थिर स्थान पर व्यवस्थित करें।
2. 0.1 एन एचसीएल तैयार करें
3. 0.1 एन को सहली हैमोमीटर ट्यूब में 2% तक भरें
4. अब दिए गए स्थान पर सहली हैमोमीटर में पिपेट रखें (दो खिड़कियों के बीच में)
5. अब उंगलियों को निर्जलित करें और नसबंदी वाले लेंस की मदद से इसे छेड़छाड़ करें और रक्त को 20 माइक्रोलिटर अंकों तक सहली हैमोमीटर पिपेट में भरें।
6. अब हस्तांतरण करें कि सहली हैमोमीटर ट्यूब में रक्त नमूना जिसमें हम पहले ही 0.1 एन एचसीएल ले चुके हैं।
7. प्रतिक्रिया होने के लिए लगभग 10 मिनट के लिए प्रतीक्षा करें।
8. धीरे धीरे धीरे-धीरे ड्रॉपवाइड अपनी तरफ आसुत पानी जोड़ें और दी गई ग्लास रॉड की मदद से अच्छी तरह से हिलाएं।
9. खून के रंग से तरफ खिड़की से मेल खाने तक आसुत पानी डालना रखें
10. पढ़ना और पढ़ना नोट करें, यह हीमोग्लोबिन का प्रतिशत है
Kon kon.meri trh practical se ak din phle ye video dekhne aaye h 😂😂
Mera to aaj hi he 🤣
@@chandnivishwakarma5889 all the best
Yes
Mein 😅
Yup 😅🥵
Just a note: The stirrer should be lifted when comparing the colors, but not completely taken out of the tube. It's so as to avoid any potential loss of fluid which would lead to a lower Hb estimate.
Yes you are right. We did a slight mistake here. Sorry for that
The explanation is good... But we need to wait for atleast 10min after adding blood to n/10hcl .. For formation of acid haematin
Yes you are right. I forgot to tell this point . Thank you
Yeah 😊
Exactly
Thank you so much. I have done this practical in my class but completely forgotten the process your video helped me to recall the process.
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Hello mam please help me mai Bmlt course me admission Lene ha rha huu
Aur mere ko Thora convence kijiye
I understand Very easy how you forgot
@@krishmohankumar5225 karo bohot Accha course hain
Exam ke ek din phle wale bachee sbse achee 😂😂😂
Doing this experiment before 30 min of viva... That's called backbenchers...
And getting knowledge on top level by @solution pharmacy..
@solution pharmacy s padhne ka ghamand hai hamko😎... Sir sirf Pharmaceutics ya Pharmaceutical chemistry ki liye bhi lecture karwa do.
I really really can't thank you enough for making this video, Prayers from core of my heart Sir.
Seham Baloch Thank you for your comment. We are happy that we could help you. Keep supporting solution
For God's and your own sake, learn the correct method from somewhere else.
Thank you so much. It was so helpful Ans very easy!!! Tmrw is my practical and you literally saved my time!!
Thank you for your kind feedback
After mixing blood with acid ,,wait for 10min and then add distilled water drop by drop
Yes
Yes
Nice pic
Right bro
nice ans.
The finger should not be squeezed to take out the blood, otherwise tissue fluid might come out,thus giving the lower estimation of haemoglobin
Yeah but most of the cases we have to squeeze the finger to get enough blood at 1 time otherwise bubble will produce which will largely effect it and the blood in capillary tube may clot in no time.
When we performed in lab most of students had to squeeze it or have to prick at different place on finger to get enough blood. With a tiny prick
Not squeezing but can gently press
right sir
This man doesn't know this simple fact and he doesn't care.
Ye sab kitabo me sahi hai , practically nahi😅
Thank you so much sir... this video is really helpful for me..... specially the details given in the description box..... 👍👍..... inshallah I will do my best in this experiment...
Thank you so much for your support and feedback. Wish you all the best dear
Kal exam hai aur aj ye video dekh rahe hai …. Internal exam hai Shayad pass ho jaye 😂😂
Thanku so much sir ur video giving me a confidence that I'll do my best in my practical class
My pleasure. Thank you for feedback. All the best
Kon kon practical ke time hi dekh rha hai
Dr,sahab aap ka bahut bahut bahut dhanyawaad
Mona here from pakistan.it is so usefull to understand experiment without confusion.
Tq sir.....kal mera phyology ka prectical he....aapka video dekhne ke Baad ab full confidence aa gya ....tq once again sir
Sanjna Rathva most welcome madam. Wish you all the best.
Tq..sir
Sanjna Rathva pleasure is always mine. Please stay supportive to solution. We are also abailable in facebook as a page.
Marks kitne aaye aapke 😅
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I enjoyed your good work I pray God to lift you higher
Thank you so much sir for giving us so much information details about this practical.
My pleasure
Thank u sir.... tomorrow's my physio practical exam
SREERAG NAIR Pleasure is mine. All the best for your exam.
🐩🐱🐱
17 gram aa Sakta he
Same here
😁path practicle here
you are doing a great job . i have also performed this practical in our college svcp indore.
thankyou and i hope you will continue the great work...
The solution is really grateful for your kind motivational comment. We are doing this for the betterment of Pharmacy students, As I was also a student and I have faced these practical problems. Thanks for your support.
Dr Pandey ने गलत सिखाया था? अब यहां से सही सीख लोगे?
Sir on the haemoglobin tube what does percentage (not Gram percentage ) denote? Today I performed this test. Gram percentage value was 9.6 and percentage value was 65%
Mashallah thats good explanation Thank you
Thank u sir for giving perfect explanation tommarrow I have practical exam I think will right well
Jack hacker All the best
Thanks a lot
Practical ke din hi dekh rahe hu😁
than you so much U and your team is great
What is the position of stirrer when you measuring the hb concentration ?
Thank u so much sir aapki vjah se meri itni bdi tension duur ho gai actually kl mera zoology practical ka exam h or mein yeh test krna bhul gaii thi ab mere ko bhi ache se smjh aa gya or mein isko kl easily lab mein Mr skti hu ty again
Nasreen rizvi My pleasure.
Me ne to sary tyari hi youtube se ki ha😅
Haematology ki zoology😅
Kon kon meri tarah practical ke hee din ye video dekhne aya h😂😉
🙋♂️🙋♂️🙋♂️🙋♂️
Thanks sir it was very helpful for pharmacy students
aapne meri bahut help ki mujhe sare process samjh aa gye thanks a lot sir🥰♥️
My pleasure
bhai thank you very very much..kal physio ka exam hai and u saved me love u
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2.3 hours baad practical h😢
mera 20 min baad
Thanks for explaining good explanation
icann't i understand Hb test before
but now i know very well
thanku sir
Asma Hassan You are most welcome sir. Happy to help you. Stay supportive. Thanks
Humlog practical lab me byathkar ye vdo dekh rahe he
Very good video....so much useful for first year MBBS students
10k subscribers without any video thank you
Sir microscope ke bare me bataiye im dmlt 2year students run..
Very helpful, thank you sir 🙏
Hello sir,
Some books also recommended filling N/10 HCL up to a Mark of 20, please explain it.
AweSome video ❤ thank u so much u clear my all confusionx
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Nice
Gudiya Kushwaha thank you
This video was very good. Thank you sir.
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Rely thax
Devanshi Pandey pleasure is mine
Thnxx sir ur video will be supported to all pharmacy student
Most wecome.
Got to know another way to fill the capillary tube thank you
It helped me in my practical exam ,(mbbs),thanks
My pleasure. Tc
@@solutionpharmacy is it b pharmacy practical or m pharmacy
Kon kon meri tarah practical ke 5min pehle video dekhne aye😂😂
Very good explanation, Nice
This is the most viewed video of the channel ..
thanku...so much...now i can easily attend my practical xam
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Pratical wale din video dekhna
Just before practical 👁️👁️ thanks
Tq uu sir very useful for our examination
Some laboratories multiply the meter reading with 8 and 9 to get the result in decimals
i,e:-
Meter reading.....13
13×8= 10.4 gms%
I still have a doubt
Hum practical wale din dekh rahe h🤣🤣
Sab wahi kar rahe 😂😂
Nice kal mera physio ka pratical he bahut madat hongi tysm
RUSHABH Tayade Thanks for your valuable response and comment. It will help us alot. All the best
thank you so much sir kl mera particle hai problem.solve kr diya......
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Dude, who's gonna wait for 5 mins after adding 20 cu mm blood in dilution tube filled 0.1 with hcl for the formation of acid hematin.
Don't mind sir, remember he is a pharmacy wala.
Very helpful information.
But u have not mentioned after placing blood in N/10 HCl in sahlis tube wait for 10 minutes
And also
In no case we don't have to take the stirrer out, add distilled water but don't take the stirrer out as it will contaminate the solution.
Yes you are correct. Sorry for these
Thnku so much, it's very helpful video..
What is the purpose of addition distilled water during estimation?? Kindly, answer..
Take 5 to 10 minats observe time than add drop by drop D. Water
swdesh sharma Exactly. We kept that for abt 8 mints but forgot to inform. Thanks
I'm pakistan practetional boy. I like your vidoes
Tahir Musarat thank you so much
Thanku sir so much ☺️ very helpful tomorrow is my exam
Sir thank you apka to samjya Hua pura samaj me agaya
Bahut achhi baat hai ye. Thank you for feedback
Thank you Sir.
Farheen Qureshi my pleasure
Please made such more videos on the other Pharmaceutical Practical topic....
Please visit channel. There are so many practical videos
@@solutionpharmacyThank you Sir Ji....
sir what should be the normal range of HB? which level to consider severe?
Bahot acha kiya hindi video bana kar......
Indian vines thank you. Socha ki isse jyada logo ko advantage milega.
Thanks! You saved me for tomorrow's exam
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Hii EXOL
@@captionjacksparrowexol7354 hi
Tq so much...
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Thank you sir it really helped for my exam 🙏
Great. Thank you so much for your feedback
Maine bhi test kiya hi sir thank you sir 🙏🙏❤️❤️
Kon Kon practical hone K 15 min phle Dekh rha h mere trh🙋🤭
Hume ye experiment na to btaya na he krwaya fir bhi dekhna pad rha h
While mixing never take the glass rod out 😐😐....
Very nice, aj me v ki hu lab me, sir ne same aisa hi btaya
Thank you. All the best.
thanku sir next week mera human phusiology ka practicle exam aa it is help full...
Sonpreet Singh Pleasure is our. All the best.
tyx sir
DNYANESHWAR KADAM welcome sir
shouldn't we wait for 8-10 minutes after adding blood in hcl ?
Yes we should wait for few mints.
yes correct...!!!!
Yes
Write
Something 10 minutes
Thank you so much sir🙏
very nice video. really very helpful.
ANUARUL MAZUMDER thank you sir
I obviously dnt knw better than u sir
But it’s not advisable to remove the stirrer everytime
Coz the blood loss occurs with the stirrer..!
P.S pls dnt get offended sir
Bt i just shared wt i knew :)
Zeel Kodia Your point is right. But we have already mixed the blood with DW so the blood volume is sufficiently maked up and it will affect the final result. Although your suggestion is highly acceptable and we are thankful for this.
Solution- Pharmacy Okay sir
Got that :)👍🏻
Waching this video just before 1hour of viva 😅😅and never done this practical in lab.
#backbencher 😂😂
This video was very good
Please can you tell me the dimensions of thin stirrer rod
DMLT wale like karo
Thanks sir tomorrow is my hap practical exam
Mai mbbs student hun, aur class ke bad bahut confused tha. Ye lecture dekhne ke bad sab clear ho gaya
Thank you for your feedback. Please watch our pharmacology videos. It will also helpfull for you.
I will definitely watch it.
Thank you sir very helpful video 👍
My pleasure
It means 20micolitre blood sample contains 13 g of hemoglobin? This micropipette is the part of sahilis meter or we need to purchase it separately
You just gained a new subscriber. Thanks a bunch sir
Thanks for the video
0.1n HCl &N/10 HCl is same? Where i can buy
Done well. About to do today 🤗
Thank you. All the best
Aaj exam hai humara 🙏🫂 aur yeh video ne apni jaan bacha di.
I am understand very well
Nice video bhot helpfull hai
Thank you
Thank you so much Sir.
Chandan Mahata My pleasure
Sir hemoglobin pipet me job blood cloting ho jata he wash kis methods me karogi
Well explained sir... Tq..
Ratna sure Thank you so much
Thank you sir...