This is the same question i received from someone in the comments on my Video on Total WBC Count. Answer is the same. So i've copy pasted the answer i gave here. Hope it helps. "The pipette is designed in such a way that the liquid in the long tube of the pipette does not take part in the dilution and mixing inside the bulb. So to get the most accurate result, the liquid within this long tube (first few drops) is discarded. Also the liquid in the long tube contains only the diluting fluid. All the cells have been sucked into the bulb. So the diluting fluid in the long tube cannot not take part in the counting and so has to be discarded. Hope that answered ur query.
since i'm not a clinical lab technician by profession, i googled your question and mostly found that a separate special reticulocyte count is done only if the usual RBC count is abnormally high. Otherwise, RBC count generally do not discriminate between the two and are both considered in the RBC count.
Basically to avoid double counting. Heres a good link www.researchgate.net/post/During_cell_counting_in_a_hemocytometer_why_should_we_not_have_the_cells_touching_the_middle_line_at_bottom_and_right#:~:text=The%20point%20of%20this%20%22rule,between%20those%202%20squares%20twice.
To avoid double counting. Check this out. www.researchgate.net/post/During_cell_counting_in_a_hemocytometer_why_should_we_not_have_the_cells_touching_the_middle_line_at_bottom_and_right
Sorry for late reply. Dilution factor = Total volume taken (blood + diluting fluid) ÷ volume of blood sample taken. In this case , it is 100÷0.5. Dilution factor is simply the number of times the blood sample is diluted (20 times)
Look on the smallest squares They are 16 on each square So,there are 5 biggest squares in which Rbcs should be counted having a total of 80 smallest squares inside(16×5=80) and there are also a total of 25 squares,the biggest ones so if you count smallest squares inside those 25 squares you get 800 smallest squares(25×16) so take; 80/400, you get 1/5 on simplification as the area counted.
Tomorrow is my physiology practical
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Thanks for the nice presentation.
May I know, why I have to discard 1st 2/3 drops from Pipette, before CHARGING the chamber?
This is the same question i received from someone in the comments on my Video on Total WBC Count. Answer is the same. So i've copy pasted the answer i gave here. Hope it helps.
"The pipette is designed in such a way that the liquid in the long tube of the pipette does not take part in the dilution and mixing inside the bulb. So to get the most accurate result, the liquid within this long tube (first few drops) is discarded. Also the liquid in the long tube contains only the diluting fluid. All the cells have been sucked into the bulb. So the diluting fluid in the long tube cannot not take part in the counting and so has to be discarded. Hope that answered ur query.
@@TheSingtangpaScienceGuy a lot many thanks to you sir.
Apologies, because I missed that, "my question and it's answer" was already done!
Thanks again
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Humerus anatomy
Thank you sirr 🤩i like your acsent 🙏🙏
It’s so clear thank you
Sir best 👌 👍 explanations really appreciable 😇😇
2 hours Remaining from my Ospe/Viva And I am Watching this Video😂
Very useful vdo👍🏻
My RBC count is 7.4 should i worry ?
The Blood test is all within normal rage except the RBC.
Any medical lab scientist students here?
G
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Any nutrition and dietatics students 🥳🥳🥳
Ur explanation is too good and very helpful ,tq and keep it up
Wonderful.
How accurate is this approach to obtaining RBC counts as compared to automated methods.
Does this approach have any superiorities?
Human error is not involved in chemistry analyzers. Both are equally accurate as long as the manual method is performed meticulously
nice explanation .well done
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Helpfull video 🙏🙏🙏
Why we need to discard before putting the sample in the chamber?
Does RBC count shown in lab reports also includes reticulocytes?
since i'm not a clinical lab technician by profession, i googled your question and mostly found that a separate special reticulocyte count is done only if the usual RBC count is abnormally high. Otherwise, RBC count generally do not discriminate between the two and are both considered in the RBC count.
I can't seem to suck the blood. Do we have to hold our breath for a long time?
Thank you, the video was really informative
Why should we count RBC in smaller squares based on L rule?
Basically to avoid double counting. Heres a good link www.researchgate.net/post/During_cell_counting_in_a_hemocytometer_why_should_we_not_have_the_cells_touching_the_middle_line_at_bottom_and_right#:~:text=The%20point%20of%20this%20%22rule,between%20those%202%20squares%20twice.
What did you dilute the blood with?
pls why just borders of the lower and left side are used for counting pls answer🌸
To avoid double counting. Check this out.
www.researchgate.net/post/During_cell_counting_in_a_hemocytometer_why_should_we_not_have_the_cells_touching_the_middle_line_at_bottom_and_right
@@TheSingtangpaScienceGuy thank youuuu🥰🌸🌸
@@TheSingtangpaScienceGuy pls can i have ur account to ask some quistion that realy i dont how to get la answer
How can we avoid blood clotting while take the blood sample in pipette
Use anticoagulated blood
Thank you for the video
Any student here FROM ELIXIR INSTITUTE OF PHARMACY , PUNE
Thank you 😊
Sir my RBC level is 5.91 female is their any serious problem joints pain am facing 😢
Thank you sm!! 💗
Which course is better in allied health sience
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we count the cell on upper line and on the left hand line. But u tell L rule wt is crtt sir
Any MBBS student here?
Thanks sir
Thankyou so much sir
Iam vet. Also lam learning the english pronounce from U
What do we benefit from this test to find out what
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sir why are we diluting the cells ?
To prevent cell clotting
And facilitied to count ، without adhesion
how we can find the value of "N"???
Hi, Please re-watch the calculation and formula part of the video. Its very clearly explained. N=total cell count in 5 grids.
ماذا نستفيد من هذا الاختبار لمعرفة ماذا ؟
Awesome vedio...got more clarity .sir pls can I know how to find dilution factor...pls sir pls....I have exam sir
Sorry for late reply. Dilution factor = Total volume taken (blood + diluting fluid) ÷ volume of blood sample taken.
In this case , it is 100÷0.5. Dilution factor is simply the number of times the blood sample is diluted (20 times)
@@TheSingtangpaScienceGuy thank u very much sir👍🙏🤗🤗🤗
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Who watch this one day before the exam😂❤
how did u calculate the depth factor?
1/0.1mm
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amazing.. 😄😄
Can u tell me how u got area counted =80/400
Look on the smallest squares
They are 16 on each square
So,there are 5 biggest squares in which Rbcs should be counted having a total of 80 smallest squares inside(16×5=80) and there are also a total of 25 squares,the biggest ones so if you count smallest squares inside those 25 squares you get 800 smallest squares(25×16) so take; 80/400, you get 1/5 on simplification as the area counted.
Thank you sir
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