Thank you so much 🤗 sirr, tomorrow is my practical exam is there and I am watching your videos for performing this tomorrow 🙏🙏🙏 great work. Keep it up.
شكرا لحضرتك يا دكتور بارك الله فيك وزادك الله علما أنا مكنتش فاهمه السكشن كويس فى الكليه ولكن دلوقتي افضل بكتير Thanks you sirr❤ I understood this class well. and I have practical exam next week. 😊
thank you for your amazing videos i'm a diploma pass out in laboratory technician and right now pursuing bachelors in the same and your videos are helping me understand much better. So keep up the good work👍👍
at 1:00 you said to draw the blood sample using your mouth or a syringe. Isnt mouth pipetting outdated and really dangerous? we were taught in the lab to never use our mouths to aspirate samples and use an aspiration bulb instead
Thank you so much for the video. It really helped me. I hope there is a video for Reticulocyte Count as well to complete the 3 main Blood counting Procedures.
Thanks that means a lot. Please check the links in the video description to watch my other hematology videos on RBC count, DLC , Hb estimation and more.!
Thank you for the great explanation when both sides of chamber counted to we use total square of 8? or there is no need to count both sides of the chamber ? Thank you
If you count WBCs on the central square and there is no error in your counting, would your final result tally with that obtained from large comer squares?
The pipette is designed in such a way that the liquid in the long tube of the pipette does not take part in the dilution and mixing inside the bulb. So to get the most accurate result, the liquid within this long tube (first few drops) is discarded. Also the liquid in the long tube contains only the diluting fluid. All the cells have been sucked into the bulb. So the diluting fluid in the long tube cannot not take part in the counting and so has to be discarded. Hope that answered ur query.
This is due to the fact that there is little or no WHITE BLOOD CELLS in the thin capillary tube as all the cells are drawn into the bulb by the rising diluent. Using the first few drops can give very misleading results as it will further dilute any sample which comes out of the bulb onto the counter. Sorry for not seeing this question a year ago
Extremely sorry for late reply. Dilution factor is simply the number of times the blood sample is diluted , in this case 20 times. So dilution factor is 20. Dilution factor = total volume of sample (blood + diluting fluid) ÷ volume of blood sample taken. So its 100÷0.5 Depth factor is 10, in this case . Depth factor is 10 times of 0.1mm (which is the depth of the space between the coverslip and the counting chamber surface. In order to arrive at the number of cells per cu.mm, we need to make the depth 10times because the area of a counting square is already 1x1mm, so we have to make the volume of a counting square as 1x1x1mm
@@TheSingtangpaScienceGuy nice explanation. Thanks so much. However, I think that "100÷0.5" you wrote as formula for getting dilution factor is for RBC count. In this case, (for WBC count) it is approximately 10÷0.5. Plz, correct me if I'm wrong.
The formula is : total no of cells× diluting factor(20) /area of squares ( 4×1= 4) × depth (0.1) .... Which on solving becomes no of cells × 50 I hope it helps
Thank you so much 🤗 sirr, tomorrow is my practical exam is there and I am watching your videos for performing this tomorrow 🙏🙏🙏 great work. Keep it up.
Tomorrow my practical exam 😂
Tommorrow is mine😁
@@harshadasabale682 all the best bro
Tomorrow will be mine exam too 😝
@@mehvishyousuf1850 in which clg u study of bmlt
1hour later is my physiology practical....thanks for last moment revive
شكرا لحضرتك يا دكتور
بارك الله فيك وزادك الله علما
أنا مكنتش فاهمه السكشن كويس فى الكليه ولكن دلوقتي افضل بكتير
Thanks you sirr❤
I understood this class well.
and I have practical exam next week.
😊
Fun fact :-More than 90% people watching this vedio before there practical exam
100 percent
😂😂😂
😂😂
Tomorrow 😂😂😂
😂😂😂
thank you for your amazing videos i'm a diploma pass out in laboratory technician and right now pursuing bachelors in the same and your videos are helping me understand much better. So keep up the good work👍👍
Thank you very much. This was a very important vedio for me to understand the correct technique. All the necessary steps is here.
Thanks a lot sir! Your videos are so aesthetically made and easily understandable❤️ tomorrow is my practical exam and now I’m confident 😇
Tommorow is My biotech practical paper
And here I am watching the procedure 😂🤣🤣🤣
Wish me luck
Thank you so much sir
You made it look simpler and very interesting
I really enjoyed the video
Thank you for this video, I’m using it to prepare for my presentation tomorrow
Tomorrow is my last exam so I hve to watch this as my preparation... Thanks you Sir
You're my life's saver 🏅
Great Effort..👍🏻
Bless you🌸
Thank you so mush today I have bio medical lab
❤❤❤
Thank you so much for your excellent presentation ❤❤❤
Tomorrow um gonna to have practical exam and watching your vidios as a preparation for it.
I'm DVM
Thanks🙏
You just help a brother!!
Thank you so much
at 1:00 you said to draw the blood sample using your mouth or a syringe. Isnt mouth pipetting outdated and really dangerous? we were taught in the lab to never use our mouths to aspirate samples and use an aspiration bulb instead
Thankyou sir what a explanation I understand everything thing my University college teacher taught mai wrong way to do this ❤❤❤ love you sir
Great explanations 🥰🤍
شكرا جزيلا ساعدتني بفهم التجربة جيدا لأداء امتحاني بشكل ممتاز
Really good explanation
Thank you so much for the video. It really helped me. I hope there is a video for Reticulocyte Count as well to complete the 3 main Blood counting Procedures.
Thanks that means a lot. Please check the links in the video description to watch my other hematology videos on RBC count, DLC , Hb estimation and more.!
Ollllllkkki ppmm
Doesn't the " leave lower left " rule mean that we shouldn't take into account the wbcs lying at lower and left position?
Didn't you say the opposite?
Amazing great. This video clear my concept's thanks you
Amazing content🤗 🤩 thank u
Amazing explanation sir proud of you ♥️😊😊
Thanku so much sir , you have explained very well. Outstanding 👍
Thank you so much Sir ❤❤
Amazing video sir🔥🔥
Thank u soo much sir this video is very helpful to us.
Thank u so much sir ur good information we have exam tomorrow
Thanks for the lecture
So interesting and amazing
Thank u sooo much sir.... honestly it was really helpful for me
Thanks tomorrow is my practical exam i am waching her
Good day sir. Why do you multiply by 50?
Great video sir 👍👌👌 thank you
Thank you for the great explanation
when both sides of chamber counted to we use total square of 8? or there is no need to count both sides of the chamber ?
Thank you
We're did you buy the kit from
We appreciate your effort, thank you, it's great
Im from jordan
If you count WBCs on the central square and there is no error in your counting, would your final result tally with that obtained from large comer squares?
Plz, will you help me that , from where we have to start counting in "L" marking .how many boxes we have to count
Was of a great help... Thanks a lot
شكرا انا وصديقتي جنت اقرره وعدنه امتحان عملي غداا
Why few drop is discarded before charging ??
The pipette is designed in such a way that the liquid in the long tube of the pipette does not take part in the dilution and mixing inside the bulb. So to get the most accurate result, the liquid within this long tube (first few drops) is discarded. Also the liquid in the long tube contains only the diluting fluid. All the cells have been sucked into the bulb. So the diluting fluid in the long tube cannot not take part in the counting and so has to be discarded. Hope that answered ur query.
Can any of the door slides be used?
Do we have to put our fluid between coverslip and chamber.?
Thank you so much😍😍😍
what is the color of diluting fluid are you using??
thank you so much💘💘
Co aaamii
Thankyou so much .❤️❤️🙏
Nice sharing 👍
Preparing for evaluation from this
@@topclasshobbies981 goodluck
What is 0.05 in the leukocyte formula ,total count=N×0.05×10^9/L
Thank u for your good explanation
Allah bless you for this
Thanks 😊😊
Good explain 🥀🥀
My kind regards
Thank you very much ❤️
Tq sooooooo much 🙏❤️
Why the number of WBC Multiplied with 50 ?
How its has 20 times dilution I can't understand that ?
How we can calculate N ?
Why does the acetic acid destroy rbc but leave wbc intact ?
good job 🎉🎉🎉 but try sir to explain more slowly please 🙈🙈
Can the pipetting be done without the mouth
Use insulin syringe to aspirate.
Thank you sir👍
thanks for your efforts
Thank you so much sir
god bless you
Thanku sir 🙏🙏
Very helpful 🤗
Why first few drops are discarded ??
This is due to the fact that there is little or no WHITE BLOOD CELLS in the thin capillary tube as all the cells are drawn into the bulb by the rising diluent. Using the first few drops can give very misleading results as it will further dilute any sample which comes out of the bulb onto the counter. Sorry for not seeing this question a year ago
Plz explain a bit more about depth factor n dilution factor.🙏
Extremely sorry for late reply. Dilution factor is simply the number of times the blood sample is diluted , in this case 20 times. So dilution factor is 20. Dilution factor = total volume of sample (blood + diluting fluid) ÷ volume of blood sample taken. So its 100÷0.5
Depth factor is 10, in this case . Depth factor is 10 times of 0.1mm (which is the depth of the space between the coverslip and the counting chamber surface. In order to arrive at the number of cells per cu.mm, we need to make the depth 10times because the area of a counting square is already 1x1mm, so we have to make the volume of a counting square as 1x1x1mm
@@TheSingtangpaScienceGuy nice explanation. Thanks so much. However, I think that "100÷0.5" you wrote as formula for getting dilution factor is for RBC count. In this case, (for WBC count) it is approximately 10÷0.5.
Plz, correct me if I'm wrong.
Excellent sir
? Why we uousing diluting solution
Thank you …..but why we multiply in 50
What 50 consider to cross it with sum of No.of cells in all 4 squares
The formula is : total no of cells× diluting factor(20) /area of squares ( 4×1= 4) × depth (0.1) .... Which on solving becomes no of cells × 50
I hope it helps
Why multiply by 50?
thank you sir
Please can you send me in writing to report this practical work
😂😂😂 are you serious? write it yourself
Med student from Ohio
This is amazing
Why is depth factor 10
Low power microscope means 10x or 40x???????????
10x obj
5:23
This Video save me thanks
You will tent the pipette not shake bz destroying of wbc occures by shaking it
thank you so so much
Good content.thank u
Anatomy & physiology
Thank you
Man ur an angel
Thank you sir
الف رحمه على والديك
Jenkins Street
Thanks
Why i am here? Cause Only 3 hours left for my exam.
30 mins 😢
I have just 22 mins to my exam😅😅😅
Remington Lights
Schumm Turnpike
Informative
Everardo Tunnel
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Jacquelyn Square
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Thank u sir