How do we load in this type of chamber? There seems to be no groove unlike some other versions where there is a U shaped groove to place diluted sample near the counting chamber.
Mainly because of 1. difference in abundance of RBCs and WBCs. 2. size difference between the two. 3. Counting time The central squares are subdivided into tinier squares. Even the tiniest squares contain a significant number of RBCs. So it will be a waste of time counting RBCs in the larger squares. WBCs on the other hand, are comparatively much much fewer in number than rbcs. So if u count them in the central smallest squares, there is high chance that you might even end up counting Zero wbcs even after counting , say, 5 squares. The chances of observing and counting wbcs in the largest squares is much higher and is also gives more accurate result.
Therefore as a general rule of thumb, the central 5 smaller squares are allotted for rbc count while the peripheral Largest squares are devoted for wbc count. The same goes for sperm count. They are very abundant per drop of semen sample therefore central small squares are used for sperm count. Semen analysis bytheway is my future video. Do stay tuned.
You are the reason i passed my cell biology practicals ❤️
How do we load in this type of chamber? There seems to be no groove unlike some other versions where there is a U shaped groove to place diluted sample near the counting chamber.
It’s so informative, thanks man❤❤❤
how do you clean the pipette (rinse)and the hemocytometer after use? with what?
with alcohol 70% followed by a final rinse with 100% alcohol or Acetone. If stubborn blood stain inside, H2O2 is an option, before the alcohol rinse.
Why ? The central square only is used to count RBCs and the peripheral 4 squares for counting WBCs ???
Mainly because of
1. difference in abundance of RBCs and WBCs.
2. size difference between the two.
3. Counting time
The central squares are subdivided into tinier squares. Even the tiniest squares contain a significant number of RBCs. So it will be a waste of time counting RBCs in the larger squares.
WBCs on the other hand, are comparatively much much fewer in number than rbcs. So if u count them in the central smallest squares, there is high chance that you might even end up counting Zero wbcs even after counting , say, 5 squares. The chances of observing and counting wbcs in the largest squares is much higher and is also gives more accurate result.
Therefore as a general rule of thumb, the central 5 smaller squares are allotted for rbc count while the peripheral Largest squares are devoted for wbc count.
The same goes for sperm count. They are very abundant per drop of semen sample therefore central small squares are used for sperm count. Semen analysis bytheway is my future video. Do stay tuned.
@@TheSingtangpaScienceGuy Ohkay . Thank you so much for the explanation ❤️😊💫.
Very nice explanation 👍
Thank u so much.🤗🤗
Thanks for the amazing explanation
Tq sooooooo much 🙏❤️
thankyou ❤️
Thank you so much
sickle cell anaemia ka pur prtical
sickle cell anaemia of screening ka video sand karo abb
Thanks lale
hello sir
Your voice is so low