Leishman Stain Procedure and Blood Smear Preparation for DLC Count
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- Опубликовано: 4 сен 2024
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Please don't squeeze the finger while drawing capillary blood as the tissue fluid oozes out and mixes with the blood thus diluting the blood sample giving false results.
Yaar lekin squeeze nahi karte to blood nikal ta hi nahi
@@_ABHISHEKSAHA_MBBSO- Uske liye needle 45 degrees angle par pakadkar 2 to 3 mm deep prick karo
@@tanmayeenalavade394 thanks, I will try it in my next hemat practical
Bilkul Correct bola apne
We have to do DLC not TLC or TEC
First i want to thank you a lot for this video, it helped me learn a lot!
And secondly I want to ask,
Why don't we put a cover slide on the specimen before putting it under the microscope?!
Hope you answer me soon!
On doing DLC there is no need of using cover slide. Hence its oil immersion 100x lens is used. The lens should touch the oil
After counting we have ti rub the tip of lens using spirited cotton
Good Job sir 👏 ( Morning me mera practical exam Hai 😅)
Sir we never take first drop of blood ....we have to wipe it
Yes true we have wipe the first drop
First drop of blood is discarded right ✅
Thank you sir
Thank you so much for your effort
Lesmain dalne k 2 Min bad buffer solution 2-3 drop dalte h . Fir 5-10 min k bad use tap water se dhona h Uske bad tissue se sukha k use microscope k liye ready krte h
Kaha se ho
Boht acche se bataya shukriya 🖤😃👏🏽
Thanks sir from Bihar🙏
My pleasure
@@MLTLabManual a
Thanks sir... This help me alot🙏
Thanks a lot 💓
Very Helpful video 🙋🙋
No need of methanol fixation for Leshmin stain, its alcoholic stain
Yes
Right
Right
Thank you so much sir🙏
Sir.. can I take a short part from this video to make a lecture for kerala higher secondary examination?
Nice ayit 🤣
Just found your video... The best video.. Sir can you please explain how to use oil immersion.... Please😫🙏🙏
high power me dekhne ke liye...
normal lens se 100 times extra dikhta h oil me
Just take a drop of imersion oil on smear and watch it under a microscope 🔬 simple
This method is very egy 😊
You can mix it by blowing also
Thank you so much sir
thank you so much for this video
Thank you very much sir ji
My pleasure dear
Great explain//.👍🏻
Thank you
Thank you for explaining
Hai yarrrr krta to haii😂😂
Maanta h ki nhi
Sir washing method galat hai Direct flow of water pe nhi finger pe water girne dete hai phir slide pe
Thank you sir ❤
What is the color of Leucocyte and malarea and trypanosmoma
When we use the lieshmin stain?
We dont have to rinse it completely with distilled water na otherwise the slide will be washed up completely and we will get negative results so its better to put the thumb on the slide so that it shouldn't rinse completely otherwise it will show wrong results
Thanks for your feedback
Why you are not adding cedarwood oil
No need
Nicely explain
Nyc explanation 😊
Good experience
Thanks.
Good explanation bro
Very well explained
Thank You & Please share it in your WhatsApp contact
M. P. Stain reagent A and b hota hai sir red or blue konsa use pehle hota hai btaye
Very very Thanks Sir
Tomorrow my exam thank god 😅
Best Wishes
Nice presentation 🤩
Super explain sir
How to prevent rouleaux formation ??
Make thin smear to avoid Rouleuxe formation
Thanku so much sir🤗
Nice sir
Thank you sir 😇🙏🏻
Patna में कोई Lab या teacher बताइये जो manual काम सिखाते हैं
No Idea
You forget to give Cedarwood oil on glass slide.. After that you can view under microscope..
Very helpful
Thanks sir
Thank u sir ☺️
Thank you sir
Very nice
Thanks 🙏🏻
वीडियो countinue डालिये
Sure! Dear
Mstt
Wrong air bubbles nehi hona chahiye
Hamko rbc or wbc Dikhta nahi hai mere pass Lishman Aaur Distil water hai Kya kami hai Bahut try kiye hai
Thanks 🥰
Nice video
Regular video daliye sir ji
Ok dear
But you did not add the buffer!
Metanol sei fix bhi karni hoti hai slide
Leishman me nhi krte hai, wo giemsa stain me krte hai
Ek bar ye bataiye ki slide ko kaise dekhenge... Kitne me observe karna hai..... Please tell..
video me dikhaya hu...pehle 40X phir 100X
@@MLTLabManual alright...
Aapke pas itna knowledge hai , thats great..
Mujhe bhi sikhado😌
Me interviews de rahi hu aaj kal..
Where r u working?
100x pe
Good
STAINING ROD TO KHARID LE ESR ROD PE LAGA RHA HE STAIN
Smear dry ayyinaka direct ga stain chesestharu kadha mari fixation enti process lo adhi ledhu kadha
Distilled water kitne drop daalna hai wo bhi bataaea
Leishman stain ke double volume
Great explained thanks sirji🙏
Index finger he ku use krte h prick ke liye hmesha ...!!!
Methanol kitna percent use karte hai sir
Nice one***
Direct water nahi dalna hota hai stain slide par
Right
Sir aap finger name galat bol rahe ho ek middle finger hai ek ring finger
Sir what is difference between distill water and normal saline
Distilled water is the pure form of water which is made heating , boiling, cooling vapours, that is a hypotonic solution but saline made my adding sodium chloride in water which is isotonic solution use for IV fluids.
@@MLTLabManual thnx sir
Eosin me to dip kiya hi nhi .... TLC ke liye Leishman & Eosin dono me dip karna hota hai.
not necessary
@@MLTLabManual where it is mentioned that it is not necessary to dip in eosin.
Oo bhai leishman stain contains eosin dye and methylene blue
Nice sir g
Plz determine platelets DLC 🙏🙏🙏🙏🙏 Sir
Hme edta blood lena hota aa na ki finger prick
Bhai methanol me kyun fix karte hai ? Bina uske direct stain kar sakte Hain?
Han kr skte hai ..lekin smear ko fix krne ke liye methanol me deep krte hai
Thanku sir
Distilled water se ku tap water use kr sakte hai
Yes
Yes it can be with tap water
Sir hm smear 100x pe dekhte hai na 🙄
Tlc count batyega bistsr se
Don't squeeze the finger tip
Sir ek request hai . Meri slide may black deposit bahut Jyada aate hai.. .agar dobara stain dal kr wash krti hu to platelets nahi dikhte... Plzzz help me Mai kya kru.... Hmari doctor roz bahut Jyada dantti hai Muje... Plz help me 😥😥
Black deposit aane ka matlab stain puri tarah spred nhi ho rha ..dilute proper kro ...ek baar se doctor se hi puch lo ki sir kaise Krna h ek baat bta do
Put the tissue paper on slide then pour the Leishman stain
Your are wrong don't wash the slide directly in tap water because the stains will washed out and your slide also like that
Smear pe direct aani kon dalta
Don't waste the slide directly with distil water ❌
Tq sir🙏
TLC count kese kare
How to count the dlc smear
But i can not do perfect smear. I also trying 😓😥. I am bmlt 3rd year student
Keep trying
Sir methenol se fix kiu karte hai smear ko
Acetone free methenol
Blood smear are correct.
Ek se 2 mint tak daal ke rakhte hai hum stain
Sir , ya video SRM hospital ka h Na
Nhi ..mere lab ka h
@@MLTLabManual hlo sir dlc ki + kasie krna hai
👍👍👍
Aap kahan se ho
Blood squeeze kar ke nahi nikala jaata h
Mithenal me Dena jaruri hai sir
Mandatary nhi h
GRA kay hai Sir
Sir आप पटना से है क्या
Madhubani