@@PlantCellTechnology is the traps falling off? from other articles they usually trim off the traps as they don't sprout new growth right? btw, from some articles, after the h2o2 / vinegar process, some ppl usually cleanse with sterile water, is that advisable? I mean h2o2 / vinegar on the ms media not good right? Thanks for all your awesome Videos!
@@momomeshtv7318 Yes, you are right! It's recommended to clean your explant, after treatment, with sterile distilled water to remove any chemical residue.
@@PlantCellTechnology it seems I had to fail, to learn, threw the plant tissues into the media without cleaning off h2o2 the first time following your videos, and the tissue just melted away into oblivion... HAHA!
What is the composition of the medium to grow Venus flytrap? Also, I see every plant you serialize with 1% bleach for an hr and then use 3% hydrogen peroxide ending with vinegar for 5 min. But for me every time I used 3% H2O2, the plant turned dark brown after few hours. How can this be prevented? Am I doing something wrong?
Oh! we haven't made an update however plants are thriving well in our labs. Will make a video on them soon in near future. So amazed to see your interest!!
@@maho9369 Hey Ma Ho, currently, we are working on many other different topics of tissue culture, as requested by many other viewers as well. So, didn't get the chance to make an update video on the plants. However, I will convey your words to Francisco for the update on the plant. Thanks!
Does anyone have advice on how to cut the venus flytrap explants( or not cut)? So far I did get some clean explants that atleast stayed green for the past 4 weeks, but they dont seem to form any new shoots or even callus. i used traps cut on both sides and pieces of petiol.
We do teach the skill in our master class. If you're interested, you can join the next one: plantcelltechnology.com/pages/master-classes The great thing about the class is that it's online, so you can ask as many questions as you like about tissue culture plants.
From building your lab (in case you don't have any) and equipping it with various equipment and tools, you need at least $500-$700 to get started in tissue culture.
@@PlantCellTechnology gotnthis from Internet save the search your end. Preparative Methods: prepared in the laboratory by reacting Acetic Acid with hydrogen peroxide in the presence of catalytic quantities (1% by weight) of Sulfuric Acid ; when 30% H2O2 is used the concentration of the peracid reagent obtained is less than 10%.
Hi Luis, the plants are thriving well in our lab and we have even subcultured and multiplied them into more. However, with his other tasks at hand Francisco couldn't make an "update video" on it.
Hey Lyons, I have been really busy teaching about using the new temporary immersion bioreactors and setting up my lab. But, I'll soon provide the update on my cultured plants. Thanks!
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Any update on this experiment.
Any update on these?
any updates?
We'll let you know as soon as we'll have our video on the same!
@@PlantCellTechnology is the traps falling off? from other articles they usually trim off the traps as they don't sprout new growth right? btw, from some articles, after the h2o2 / vinegar process, some ppl usually cleanse with sterile water, is that advisable? I mean h2o2 / vinegar on the ms media not good right? Thanks for all your awesome Videos!
@@momomeshtv7318 Yes, you are right! It's recommended to clean your explant, after treatment, with sterile distilled water to remove any chemical residue.
Thanks for watching our videos! Stay tuned!!
@@PlantCellTechnology it seems I had to fail, to learn, threw the plant tissues into the media without cleaning off h2o2 the first time following your videos, and the tissue just melted away into oblivion... HAHA!
What is the composition of the medium to grow Venus flytrap?
Also, I see every plant you serialize with 1% bleach for an hr and then use 3% hydrogen peroxide ending with vinegar for 5 min. But for me every time I used 3% H2O2, the plant turned dark brown after few hours. How can this be prevented? Am I doing something wrong?
lookup: oxidisation
wow I am really curious about the results of this experiment. Please make a video to show us the update
Sure! We'll notify you as soon as we make the update video.
@@PlantCellTechnology so no results yet after a year?
@@hammad3180 The plant grew well, and we even used it to multiply more plants from it. However, we missed creating an update video.
@@PlantCellTechnology wow thats awesome. Please do make an update video once you are free.
@@hammad3180 Sure! We'll ask Francisco to give a tour of his tissue-cultured carnivorous plants.
update when????
Please give an update on this and thank you for the videos !
Oh! we haven't made an update however plants are thriving well in our labs. Will make a video on them soon in near future. So amazed to see your interest!!
@@PlantCellTechnology Have you updated yet?
@@maho9369 Hey Ma Ho, currently, we are working on many other different topics of tissue culture, as requested by many other viewers as well. So, didn't get the chance to make an update video on the plants. However, I will convey your words to Francisco for the update on the plant.
Thanks!
@@PlantCellTechnology need update
@@PlantCellTechnology update pls
Does anyone have advice on how to cut the venus flytrap explants( or not cut)? So far I did get some clean explants that atleast stayed green for the past 4 weeks, but they dont seem to form any new shoots or even callus. i used traps cut on both sides and pieces of petiol.
We do teach the skill in our master class. If you're interested, you can join the next one: plantcelltechnology.com/pages/master-classes
The great thing about the class is that it's online, so you can ask as many questions as you like about tissue culture plants.
Update when
update when
its been a year how is it?
Whats the medium you used? I don't think you said anything anout it when you placed the plants in
It's 1/3 MS media without hormones.
What’s the cost of this project?
From building your lab (in case you don't have any) and equipping it with various equipment and tools, you need at least $500-$700 to get started in tissue culture.
Is the 3% h202 at 200ml with the white distilled viniger at 1ml called Parasitic acid?
Need to confirm this with Francisco. Will get back to you as soon as he responds.
@@PlantCellTechnology many thanks my friend
@@PlantCellTechnology gotnthis from Internet save the search your end.
Preparative Methods: prepared in the laboratory by reacting Acetic Acid with hydrogen peroxide in the presence of catalytic quantities (1% by weight) of Sulfuric Acid ; when 30% H2O2 is used the concentration of the peracid reagent obtained is less than 10%.
@@lyonscultivars This is for?
@Plant Cell Technology it isn't bud. I was asking if you was basicly making parasitic acid by mixing the 2 compounds to make the sterilising liquid.
Any update?
Hi Luis,
the plants are thriving well in our lab and we have even subcultured and multiplied them into more. However, with his other tasks at hand Francisco couldn't make an "update video" on it.
Aby update after 8 months pal
Hey Lyons,
I have been really busy teaching about using the new temporary immersion bioreactors and setting up my lab. But, I'll soon provide the update on my cultured plants. Thanks!
@@PlantCellTechnology thank you for the reply. Look forward to it
@@lyonscultivars Sure! Let us know if you have any other concerns. Happy Culturing!!