You just used MS and sugar to make the media + Gellan Gum. I was using Gellan gum with MS + Sugar, and it wouldn't gel post-autoclave because there weren't enough divalent cations (Ca2+) in the solution. When I added 3 mM CaCl2 to the media, it gelled as expected post-autoclave. What type of MS media are you using in this video? Does it contain vitamins or some source of cations?
Can't wait for the next video , that would save so much time.
You just used MS and sugar to make the media + Gellan Gum. I was using Gellan gum with MS + Sugar, and it wouldn't gel post-autoclave because there weren't enough divalent cations (Ca2+) in the solution. When I added 3 mM CaCl2 to the media, it gelled as expected post-autoclave.
What type of MS media are you using in this video? Does it contain vitamins or some source of cations?
Question ..dose one philodendron start produce 1 plant via tissue culture?
Have you multipled cannabis and if so would you have a protocol
Hi, is it my english or you said UV-B ?! Could you try it with UV-C light ?
Hi. In the video, we just mentioned UV light. Also, in labs UVC light is the most widely used one for sterilization procedures.
so still waiting
Hey Gillvy - Im sorry, whats going on? What are you waiting for?
@@PlantCellTechnology i wait Part 2 ( result )
Que bien!
Is it ok to put the media bottles before culture into uv for over night
Are you talking about the sterilized ones? Though generally, 30-60 minutes of UV exposure is enough for sterilization.