It is important to note that oxaloacetate cannot cross outside the mitochondrion. It has to be converted to Malate, which will be transported to the cytosol via Malate-Aspartate Shuttle. Malate (now in cytosol) is then converted back to oxaloacetate (because of high amount of NAD+ in the cytosol) allowing phosphoenolpyruvate kinase to catalyze it into phosphoenol pyruvate. 😊
4:38 The enzyme used to convert Phosphonenolpyruvate to Pyruvate is Pyruvate Kinase not PFK1. It's written in biochem textbooks and can also be found online when you search for step 10 of glycolysis
very helpful and simplifying, but please avoid mistakes such as the pfk mistake in the step 10 of glycolysis. this could be misleading for students who are having an exam. at least put a correction in the comments or the discription.
Sir I am watching your channel from last 1year I really love the way you share the topics to us,becouse you have been teaching us sir I have started my channel on RUclips just getting inspiring from you i started my channel last weak only,I really thank you a lot for making me inspire sir.
I think glucokinase which converts Glucose to Glucose 6 phosphate, only can be found in the Liver. Usually, Glycolysis can occur in all cells, so Hexokinase is the one that would replace glucokinase.
You should also mention that Glucose to G6P is converted by Glucokinse/Hexokinase as Glucokinase is only present in hepatocytes and B-cells of pancreas.
Glucose is converted to G6P by enzyme Kinase and it's called the phosphorylation step(1st step) and ADP is converted to ATP as it is energy investment phase with the cofactor Mg2+
You should redo this video with the corrections because it really is very good on how your layout things so simply. It is excellent so I suggest you re- do the video with corrections.
I have a doubt. When glycerol is used as a substrate, gycerol-3-phosphate is formed and it gets converted to dihydroxy acetone phosphate for reverse reaction. Can't the glycerol-3-phosphate directly participate in the reverse reaction?
I was under the impression that gluconeogenesis could be classified as the creation of new glucose from non-carbohydrate sources - i.e., shuttling fructose into gluconeogenesis at the liver so it can be released to the rest of the peripheral organs as glucose.
How often and in what amounts does this process actually happen during fasting? I've hear of gluconeogenesis happening with amino acids, but I think fatty acids actually convert to ketone bodies, not glucose, during fasting that lasts more than 24-48 hours. I'm willing to be that this reversal of pyruvate back to glucose happens on a very small scale, or maybe a temporary emergency situation when glyocogen stores are extremely low. I'm also willing to bet that muscle tissue used for energy is low as well during a fast. It only makes sense that during fasting, the body is smart enough to utilize the stored fuel in adipose tissue as it's primary source.
Also, when it comes to fatty acid metabolism; two glycerol backbones of triglycerides can form one glucose molecule. Perhaps this is what you mean't by fatty acids being converted into glucose. This form of gluconeogenesis happens on a small scale as well, but it does portent that dietary carbohydrate intake may be completely unnecessary.
When phosphoenol pyruvate will convert to pyruvate enzyme should pyruvate kinase......you are saying phosphofructokinase So can you plzz guide what it should be sir...
Oxaloacetate is impermeable to mitochondrial membrane it is converted to malate and then transported to cytosol and oxaloacetate is regenerated by malate dehydrogenase enzyme
Do we only do Gluconeogenesis from Amino Acids when we fast? I’m currently trying to figure out how we react with excess amino acids taken into body And some people said it will convert to fat and some said don’t Any idea?
It is important to note that oxaloacetate cannot cross outside the mitochondrion. It has to be converted to Malate, which will be transported to the cytosol via Malate-Aspartate Shuttle. Malate (now in cytosol) is then converted back to oxaloacetate (because of high amount of NAD+ in the cytosol) allowing phosphoenolpyruvate kinase to catalyze it into phosphoenol pyruvate. 😊
Thnxx for the information ❤
Thank you very much
Ok I'm here to learn a bit about a low carb high fat diet and I have absolutely no idea what you're talking about 🤣
Very clear description. Thank you
True Aiza....thumbs up 😌 for being keen
4:38 The enzyme used to convert Phosphonenolpyruvate to Pyruvate is Pyruvate Kinase not PFK1. It's written in biochem textbooks and can also be found online when you search for step 10 of glycolysis
100%
Yeah..fuck this video.
Yes bro you are correct actually small mistakes by him during editing,and copy and paste mechanism
Yes
you're right.
Hexokinase also converts glucose to glucose 6 phosphate. Glucokinase does the same job but specifically in the liver. Thanks for the video 👍
Glucokinase is 4th form of hexokinase. So you can also say it hexokinase.
@@Natasha_Mirza yeah i was about to reply with that
Hexokinase is an enzyme that help glucose convert into fructose 6 phosphate
wait the enzyme which converts PEP to pyruvate is pyruvate kinase not phophorfructokinase
u ar correct..its not PFK
yes you are right!!
terrible error!
Saria Sultan good eye, good eye
I saw it even me right now
I saw it and I rolled down to see an explanation and here you are lol
Found it on the last day of preparation. Helps a lot :)
Can you make me understand....in more simplified way..
very helpful and simplifying, but please avoid mistakes such as the pfk mistake in the step 10 of glycolysis. this could be misleading for students who are having an exam. at least put a correction in the comments or the discription.
no donations no corrections,correct ion can be done at glucokinase/hexokinase,phosphofructokinase/pyruvate kinase, malate/oxaloacetate shunt. huh.
Your channel makes my concepts always clear. This is really superb way to understand anyone.
Sir I am watching your channel from last 1year I really love the way you share the topics to us,becouse you have been teaching us sir I have started my channel on RUclips just getting inspiring from you i started my channel last weak only,I really thank you a lot for making me inspire sir.
Channel name ?
Very helpful. Explain the topic in a very crisp and profound manner.
Thank you.
I think glucokinase which converts Glucose to Glucose 6 phosphate, only can be found in the Liver. Usually, Glycolysis can occur in all cells, so Hexokinase is the one that would replace glucokinase.
I like your videos. They are short and easy but please don't make mistakes in enzymes or steps.
ههههههههههههههههههههه صح
4: 38 it is Pyruvate kinase not phosphofructokinase. BTW good 👍video.
4:38
Thank you very much sir. ❤ from Sri Lanka
Most welcome!
Best explanation in short time every found in internet. Thanks for explaining once more about Glycolysis
You should also mention that Glucose to G6P is converted by Glucokinse/Hexokinase as Glucokinase is only present in hepatocytes and B-cells of pancreas.
And also...the oxaloacetate inside the mitochondria is first converted to mate which is transferred to cytoplasm where it again become oxaloacetate
thanku so much. this video helped me to understand gluconeogenesis.
From somalia thnks , for u lecture, it is very nice
Pyruvate-->Acetyl coA-->OAA-->Malate(crosses mitochondria) ---> PEP ( in the cytosol)
Verithanam 🔥🌟
Thank you so much sir from Maharashtra
Itz. Amazing Lecture __100% understanding thnks to MedSimplified
best channel ever
This is the best I have ever seen
4:55 pyruvate is directly converted to oxaloacetate or is first converted to acytyl coA then oxaloacetate as in krebs cycle
Glucose is converted to G6P by enzyme Kinase and it's called the phosphorylation step(1st step) and ADP is converted to ATP as it is energy investment phase with the cofactor Mg2+
Such a great videos...splendid..
May u get success in every field.. Plz do it on anothers topics of biochemitry.
Great outline of this pathway sir👏👏
Thankyou ! Great revision for my metabolism exam 😄
+TheMGPforlife most welcome
sir this video is helpful for mbbs ?
Thankyou soo much !!!!! U really help in understanding biochemistry easily 💛
You should redo this video with the corrections because it really is very good on how your layout things so simply. It is excellent so I suggest you re- do the video with corrections.
I took your advise to the heart brother. New videos on carbohydrate metabolism coming in a few weeks ok. 😉😉😉😉😉😉
MEDSimplified thank you for teaching
Great work broda.👏👏😍
HELPED ME A LOT THANKS MAN!
man this stuff is like better than khan academy, very nice work
Thanks a lot sir...Its really very helpful.. 💖 JazakAllah JazakAllah 🙏
cute you are muslim
The best explanation 👍🏻👍🏻👍🏻👍🏻👍🏻👍🏻👍🏻
Thaaank you very much
I like your videos so much
Best wishes
thank you for this!! saving me in my macro metabolism class!
Dear Sir, your videos are excellent. Please make a video on glycogenolysis also.
I have a doubt. When glycerol is used as a substrate, gycerol-3-phosphate is formed and it gets converted to dihydroxy acetone phosphate for reverse reaction. Can't the glycerol-3-phosphate directly participate in the reverse reaction?
Thanks it's very sufficient and helpful
Wah!!! What explanation it easy to understand and write by own thanks alot sir ur explanation is superb 🙏
Best teaching method 👍
After from mitochondria where the oxalo go?
Made it clear , voice was good too ☺
Your lec are best ... 👍👍
Can you upload glycogenesis, glycolysis, etc and hmp shunt?
+Aishwarya Birje U can find the video on glycolysis but j our channel. Glycogenesis and hmp shunt will also be uploaded soon mam
MEDSimplified Glycogenolysis* 👅 my bad!
MEDSimplified and thanks!
MEDSimplified hmp shunt pathway ?
very easy to understand the concepts and steps...pls add more topics
This video help me in my project 😊
You explain in the simplest way possible..just one request please try to cover the portion in details
Why did you not discuss Regulation of gluconeogenesis?
انت مبدع شكرا جزيلا لك from yemen thanks
I like your videos sir. These are helpful
Please make a video on pentose phosphate pathway
I was under the impression that gluconeogenesis could be classified as the creation of new glucose from non-carbohydrate sources - i.e., shuttling fructose into gluconeogenesis at the liver so it can be released to the rest of the peripheral organs as glucose.
How often and in what amounts does this process actually happen during fasting? I've hear of gluconeogenesis happening with amino acids, but I think fatty acids actually convert to ketone bodies, not glucose, during fasting that lasts more than 24-48 hours. I'm willing to be that this reversal of pyruvate back to glucose happens on a very small scale, or maybe a temporary emergency situation when glyocogen stores are extremely low. I'm also willing to bet that muscle tissue used for energy is low as well during a fast. It only makes sense that during fasting, the body is smart enough to utilize the stored fuel in adipose tissue as it's primary source.
Also, when it comes to fatty acid metabolism; two glycerol backbones of triglycerides can form one glucose molecule. Perhaps this is what you mean't by fatty acids being converted into glucose. This form of gluconeogenesis happens on a small scale as well, but it does portent that dietary carbohydrate intake may be completely unnecessary.
very useful video at last I got it thank u so much medsimplified...
When phosphoenol pyruvate will convert to pyruvate enzyme should pyruvate kinase......you are saying phosphofructokinase
So can you plzz guide what it should be sir...
Does the body perform gluconeogenesis before fat metabolism??
Oxaloacetate is impermeable to mitochondrial membrane it is converted to malate and then transported to cytosol and oxaloacetate is regenerated by malate dehydrogenase enzyme
Thank you❤ so much 😊
7 minutes video worth more than my lecturer slides lmaooo
very nice. also glycerol part of fat and not fat is converted to glucose
Great explanation
Thnx dude got the notes for gluconeogenisis😉
which app are you using to make videos ?
Thank u very much.
It is a nice video .
Try to make hmp shunt also.
TQ short and relavent for mbbs
NIce Explanation Thanks
i was confused but after 7 minutes I now understand this process
thamisanqa deputy lmao what about the 04
Don't we use hexokinase to convert glucose to glucose 6 phosphate?
Isn't the enzyme converting phosphoenolpyruvate to pyruvate called pyruvate kinase?
Thank you soo much sir 🥰✌️
Isn't is supposed to be pyruvate kinase enzyme that converts Phosphoenol pyruvate to Pyruvate? :))
Do we only do Gluconeogenesis from Amino Acids when we fast?
I’m currently trying to figure out how we react with excess amino acids taken into body
And some people said it will convert to fat and some said don’t
Any idea?
Very Good 🙂
Very helpful😊
this channel helped me ALOT , much thx for u
It really helped me
4:15 hexose kinase change glucose to glucose 6 phosphate ❤
Thank u so much sir....all ur videos are really awesome and i can understand well....can u please make a video on HMP shunt pathway ??
Sir how many amino acid can be used to make glucose...?25%?33%?50%?
Superb sir thanks very much
Thank you sir 🙏
Your videos are super 👌nice.
Put videos for Amino acid metabolism and lipid metabolism. Fast As soon as possible.
Please make the video on HMP shunt pathway!
4:37 The enzyme should be pyruvate kinase. Please heed this.
Please make a video on HMP shunt, your videos are just easy and good
Phosphoenopyruvate is conveted to pyruvate by the enzyme Enolase not phosphofructokinase
Please upload protein metabolism..nucleic acid metabolism video...
Thanks a lot sir....🙏
thank you
Nyc sir
N ur voice is osmmm 👌👌👌👌
Thank you.
How many ATPs are consumed in gluconeogenesis?
qn excellent work
can u upload mechanism step involved in glycolysis wth some tricks so that we remember structure involve in reaction steps
it,s very simplicity thank you
Magnificent share I like your videos
Hey: Phosphoenol Pyruvate to Pyruvate by Pyruvate kinase Enzyme. Not phoshofructokinase as u mentioned ___"plz update soonly