Hey thanks a lot for this brilliant video but I have a doubt here.... we take 2 components of the transcription factor AD and DBD and bind the 2 proteins one to each.......how can we say the function of the 2 components does not get altered when one of the proteins is attached to it? Pl help me understand
Good question! So the main criteria is that you have to use a strong transcrption factor which initiates transcription of your reporter gene. In that case here they used Gal4p transcription factor: link.springer.com/protocol/10.1385/1-59259-762-9:247
wow that's a very elegant system. Does it mean then that the proteins need to be taken up into the nucleus in order to be tested, is there limitations in terms of size and charge?
Every other source i found doesn't mention anything about Gal4AD recruiting the polymerase, they just mention that transcription happens. Do you happen to have any sources that show that GAL4AD does indeed recruit the polymerase?
OK so i have 2 questions i hope im not to late 1. how can you bind a protein to DBD or AD 2. what are the usses of this also i very much like your channel
Matevz Kosutnik thanks for your question 1. you synthesize a DNA fragment where both sequences are connected and encode a protein with is ´chimericˋ meaning a novel protein out of two individual proteins which are linked via peptide bonds. 2. you can test whether protein A is interacting with protein B (if so, you generate a reporter gene expression)
Y2H is so much easier to comprehend now. Thank you so much for covering this topic!
Thank you so much for your clear and unambiguous explanations.
This is the best Y2H video I've come across that actually makes sense!
Easy to understand explanation. Thanks Henrik's lab!
Very well explained in a super simple way. Really liked it!
Brief but clear enough to understand!
This is one of the best videos on Y2H. Thank you for making this.
Great intro into Y2H, clear and concise! Thanks!
This video is a national treasure.
Thank you very much Henrik! You are doing a wonderful job with your videos.
Thanks!
Clear, concise, helpful! Thanks!!
This was so helpful as I am doing a project right now. Thank you!!
Helped in my understanding thank you
This is the best Y2H video on youtube - so easy now to understand, thanks a lot!
Vielen Dank, das war richtig gut erklärt!!
So clearly explained! Thank you!!
Very clear explaination! Big thanks
Very well explained!!!thank you
You are a life saver. Thank you
Awesome, thank you for the clear explanation
Very helpful, friend. Thank you muchly!
Best explanation ever!
Very nicely explained.
Thank you. Wonderful explanation.
It’s very helpful, thank you so much.
Hey thanks a lot for this brilliant video but I have a doubt here.... we take 2 components of the transcription factor AD and DBD and bind the 2 proteins one to each.......how can we say the function of the 2 components does not get altered when one of the proteins is attached to it? Pl help me understand
Thanks so much for this helpful video!
danke! endlich mal ein verständliches video :D
Nicely explained. Thanks a lot! Is there a particular transcription factor used for this? Or a criterion for choosing the transcription factor?
Good question! So the main criteria is that you have to use a strong transcrption factor which initiates transcription of your reporter gene. In that case here they used Gal4p transcription factor: link.springer.com/protocol/10.1385/1-59259-762-9:247
thank you so much, so clear and concise!
finally i got it .
thank uuuu .
Allah bless you ❤
Thank you so much, ths video helps me a lot!
Excellent explanation ❤
This is a great explanation, thank you so much!
very good explanation, thanks
Great video thanks!
wow that's a very elegant system. Does it mean then that the proteins need to be taken up into the nucleus in order to be tested, is there limitations in terms of size and charge?
Richtig gutes Video, vielen Dank!! 🙏🏼
Freut mich wenns geholfen hat!
Just amazing👍👍👍👍
Nice presentation, thank you, make more videos on rdt
Well explained thanks ❤❤❤
Every other source i found doesn't mention anything about Gal4AD recruiting the polymerase, they just mention that transcription happens. Do you happen to have any sources that show that GAL4AD does indeed recruit the polymerase?
Thanks alot, it was very useful
clear very much
Thank you so much.
Thank you
Thnx a lot sir...it helped me a lot in undrstanding this concpt💖💖💖
Perfect!
Thank you it's really clear !
clear explanation
Easy to understand good job
Very clear thank you so much
thank you very much bro
OK so i have 2 questions i hope im not to late
1. how can you bind a protein to DBD or AD
2. what are the usses of this
also i very much like your channel
Matevz Kosutnik thanks for your question
1. you synthesize a DNA fragment where both sequences are connected and encode a protein with is ´chimericˋ meaning a novel protein out of two individual proteins which are linked via peptide bonds.
2. you can test whether protein A is interacting with protein B (if so, you generate a reporter gene expression)
Thank you ❤
amazing
Does the bacterial adenylate cyclase two-hybrid (BACTH) system also utilise this concept?
Hi nice video!
Hab eine Frage
Also dient dieses System lediglich für die Erkenntnis ob Protein a und Protein b interagieren?
Ist im Normalfall wirklich ein Verfahren um Interaktionen von zwei Proteinen zu checken, ja!
RNA Polymerase binds to the promoter and transcription factors bind upstream of the promoter.
Good explanation
Thank you so much!
Perfect❤
Thanks a lot
Thank you!!!
danke Bruder ich küss dein Auge fürs video
U r great
GJ!
love u
Thank you so much! I liked your video and it was at 666 likes, so sorry if i ruined it!
You did not! The goal is definitely a 1000 likes :D
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i love you
You can discuss this with my girlfriend :D
Thank you so much, it helped me so much!
Thank you
Thank you