Yeast 2 hybrid system | Yeast two hybrid system for protein-protein interaction | Animated biology
HTML-код
- Опубликовано: 19 авг 2024
- Yeast 2 hybrid system is one of the important methods to study protein-protein interaction invitro and this video explants the concept behind the technique in details.
Also follow me on other social media -
Facebook: / arpan.parich. .
Instagram: / arpanparichha
Linked in: / arpan-pari. .
Twitter : ar....
Instagram page : / animatedbiologywitharpan
Facebook page : www.facebook.c...
Link to my another channel - Lets crack bio exams / @biologyin20min
For Physics part of bio exams - Physics for IIT JAM biotechnology
/ @physicsforiitjambiote...
#animated_biology #animated_biology_with_arpan #biology #bio_facts #CSIR_NET #IIT_JAM #IIT_JAM_BT #biotechnology #bioscience #biological_sciences #microbiology #genetics #zoology #molecular_biology #cell_biology #botany #immunology #ecology #yeasttwohybridsystem
Now this was the best explanation I have had .
After watching multiple videos still I reached here to get more detailed information
Thanks for this !!
Hassa Iftikhar please share among your friends and don’t forget to check out all my playlists
Thank you for make this topic easy. 😊 waiting for many more like this.
Follow me on instagram and facebook
My social media handle is @animatedbiologywitharpan
your explanation is good, though I have got a doubt. Y2H is a in-vivo method or in-vitro method? you mentioned this technique as in-vitro. please clarify which is correct
What is invivo vs in vitro is highly contextual. In convenientional sense it should be in vitro
@@animatedbiologywitharpan as it occurs in yeast hence invivo
very nicely done. explained it in the easiest way possible
Please share my channel link with your friends
The video is great! Thank you very much! I assume it is in fact an in vivo method not in vitro. Otherwise, you wouldn't have needed all this yeast cell machinery to perform the assay.
I really don't like long videos, and you just explained in 5 mins thank you sir... My exam is a day after tomorrow 😂 and I'm here try to understand ...
Please check out other playlist for more such videos....share with your friends as well
You are my life saver 😊😊
please share my channel link with your friends
Is there a promoter expressing gal4ad? What initiates the gal4ad to bind to the dna?
Yes yes a minimal promoter is there.
Thnku so much sir fr explaining this cncpt in easy way🥰🙏🙏
Please share my channel link with your friends and help me to reach big audience
@@animatedbiologywitharpan sure sir😊
You are really awesome I really admire you😊..thnks for making each topic so easy to understand. 🎉
Please help me by sharing my contents with your friends
By the way revise your concepts using our mcq prep course only at 350 rs one time pay with life time subscription. animatedbiologywitharpan.graphy.com/courses/CSIR-NET-Life-sciences-MCQ-practice-course-645881d4e4b0c11d957360ef
Great explanation! Many thanks!
Please support my channel via super thanks. Your small contribution like 1$ means a lot for us. The super thanks option is present at the lower right corner of any video, a heart sign with $ in it.
Please share my channel link with your friends and help me to reach big audiance
Very well explained.👏👌
Please share my channel link with your friends and help me to reach big audiance
Succinct and well-explained. Thank you for your service.
~a masters student
In order to make high quality content consistently, we need support from you. Please support us by using super thanks option. Super thanks icon is present below the video ( a heart sign with $ in it ) . You can support using paytm/ phone pe/ gPay / paypal. Your small contribution means a lot for us.
Please dont forget to share with friends
Informative...thanku
Yeast two-hybrid (Y2H) screening is an in vivo method not vitro
Nice explanation using animation
Could you please help me by sharing my contents with your friends group/ college group. I put huge efforts in making these videos but unfortunately not a lot of people are watching this.
veryyy clearrr thank youu
Please please share my channel link with your friends and help me to reach big audience
Great video. Could you try making a video on translation and transcriptional inhibitors. A summary of mechanism with a few examples of toxins and antibiotics
ya ya it is requested by many people ,so I would be doing that soon
Very good one. Sir how to check the interactions through multiple dropout media say histidine, uracil trp and so
Thanks susmita for your suggestion, I will try to make a video on that. It just that I get very less time to make videos
Very good video ! Thank you !
Please share my channel link with your friends and help me to reach big audience
Great video, thank you so much!
Share among your friends and help them as well
@@animatedbiologywitharpan I'll definitely do it! keep up the good work
GREAT VIDEO! YOU SIMPLIFIED IT. PLEASE IS THIS RELATED TO YEAST TRANSFORMATION . PLEASE EXPLAIN
Transformation means delivering gene into a cell .....it is possible to do it on bacteria ,yeast or mammalian cell culture. So generally for yeast electroporation is the mode of gene delevery
Any way if you like my video please share my channel link with your friends and help me to reach big audience
I saw the video well. But I have a question.
1:41 Isn't it correct to combine AD with Fish protein and DBD with Bait protein?
Please understand what you say with a translator because you don't know English.
Sir can you please make a video on
Nanobodies as a tool for protein structure and function studies
Waiting for this 🙏
Honestly i don't have expertise on that
@@animatedbiologywitharpan ok sir thanks for your rply❤️
Excellent 😍😍😍😍
Please share among friends and help me to reach big audience
Thank u somuch for the video👏😍.. I have a doubt regarding transcription factor binding to protein , I think bait protein binds to DNA binding domain of transcription factor and fish/prey protein with that of activation domain..can u plz give a clarification?
Anek dhonyobad Bandhu….Bangladeshi Khaoar amar khub valo lage….Poddar ilish er kono tulona nai….
I am new in the molecular field
good video, I do have a question though. Why do GAL AD and GAL BD bind, when two protein interact? Coulnt the active sites interacting with eath other from the bait and the fish protein be on the opposite sides of those of the GAL proteins, which would mean that the protein interact but the GAL proteins are to far from each other to interact and activate the reporter gene? Or on the other hand isnt it possible as well to have two proteins which dont interact but the the GAL proteins still are able to bind and produce the reporter genes? Shoundt there be a large number of false postive and false negatives with this method?
It’s a very interesting question.... honestly I don’t know a definite answer... I would check the structural basis of interaction and get back to you
Sr kya aap kisi onlyn platform pe teach krte ho?? Like unaccdmy ???
Yes I have courses in unacademy. For more info email me at arpanparichha1994@gmail.com
@@animatedbiologywitharpan ok sr
seriously, all the reactions are happening inside the cells under physiological condition. how is this method in vitro instead of in vivo? doesn't make any sense.
Isn’t it an in vivo technique?
Depends what you call in vivo. Some people belive in vivo is tissue level stuff. For Some it is cell level stuff.
use pointer to point "this", otherwise very easy to understand :)
Tha js for the suggestion.....I will incorporate it in my subsequent videos
Chrome Says It's An in Vivo Technique
Some say its done in vivo
Ya depends on what you consider as in vivo
Library based screening large scale of binary interaction shows in vitro which is phage display and in vivo is Y2H
I think it's in vivo not in vitro
Its a controversy ! One school of thought suggests its in vivo, because the interaction is tested inside an organism . But current view conflicts that. Any way for undergrad textbook level, lets consider it in vivo.
I have heard that this is an in vivo method as this happens within the yeast. Isn't it so?
Just because it is happening in an yeast it's not in vivo...the terms are used in pretty loose manner . ( for example cell culture is also considered as in vitro models )
@@animatedbiologywitharpan Thank you for the clarification!
Please subscribe to my other channel ruclips.net/channel/UC4IpyopsGWSjaPACNTZLuqg
@@anaghamenon7857 Literally, what he has explained to you is not correct. Cell culture is considered to be in vitro because such method is only used to produce biological compounds such as antibodies, instead of to detect a reaction or interaction inside the cells. The majority of the experiment is performed outside of the cells. Yeast 2 hybrid system is an in vivo technique because not only all the proteins are expressed and matured inside the cells, but also the interaction. So, what you have heard before is correct!
Video is good but your voice is speed. I am not able to follow. Please speak slow.
Thanks for the feedback... but you can always change the playback speed to 1.5 x for making it slower.... may be you can try it
Please share among your friends and help me to reach big audience
This is wrong, it is an in vivo method of studying PPIs.
Great video! Could I use it for a university web project? I cannot see any details about copyrights, that's why I'm asking :)
Use it with acknowledgement
@@animatedbiologywitharpan Thank you! :)
Please share my channel link with your friends and help me to reach big audience
But this is an in-vivo technique. Also, I guess you explained the Bait & Fish concept incorrectly.
Here is the reference: - www.ncbi.nlm.nih.gov/pmc/articles/PMC2705515/