Basically passed all my exams from organic chem to molecular biology related subjects in uni by refreshing the contents in my notes through ur lectures. Keep it up sir! Well done!
We do watch your videos all the time and they are so much helpful for us in biotechnology sir. We do almost all the exams in the university by watching your videos. You are capable of teaching even the hardest parts, in simple ways. We like to watch videos which you yourself teach in the white board, very much. Thank you sir...bohoth bohoth shukriya!!
I wanted this a few times- thank you for breaking down the process of PCR. You don't waste time on unnecessary words or pauses like many "youtube teachers" do,
i am getting hope to clear exam only because of your videos. after 8 yrs gap, i started to persue my carrier, its such a long break , ur videos given me much confidence . i hats off u. u r my role model thanks shomu sir.
I am very blessed found your tutorial lecture on Molecular Biology in the you tube, you gave a simple way how to understand the application and knowledge about molecular biology, you are very kind to share your knowledge to everybody in the world.....hope you get present from the God....salam from Indonesia....
thanks for perfect videos you share they're both informative and productive. It's brilliant that we have such awesome lecturers like you in RUclips who are trying their best to inform others
Very interesting sir as you said .thank you very much for the lecture.you have provided us with very minute details which are Essential for attending the long questions ans . ❤️❤️
Hello sir, your videos are very helpful to all of us ( me and my batchmates )sir we all just watch your videos and we all have passed our exams with good Marks thank u soo much sir for making every topic so simple to understand. sir I am facing problem in plateau effect in PCR ,I am not able to understand it, so sir can you please help me out
Thank you sir for the good work .... I have understand the concept of PCR very easily through ur lecture, it's very helpful. . Please upload more videos related to this . Your explanation is very good ....
hi shomu i have a question reagarding pcr reaction.. why initial pcr product produce long and varying size product in reaction, and fina reaction produced shorter and uniform size product?
Got one question! when a gene is encoded on a single strand of DNA called template strand then why do we perform PCR on both strands of DNA using 2 primers?
hello tysm for this video.u r genius.u helped me in molecular biology n genetic engineering so much..can u plz provide me yr written material or books...???
A primer may bind anywhere on the DNA, obviously to ours complementary sequences but it's not always necessary that these sequences will be present at the very start. So the sequences where primer doens't bind how are those sequences amplified?
sir, i am confusing the order of the videos, recombinate DNA Technology 61 tutorials are there , but which one is 1st , 2nd..........61 videos, this is my confusing , i hope you pls clearify , thank u.
It binds first at the 3' end of the primer. That's why the 5 base pairs on the 3' end of the primer are very important In order to promote a proper annealing to the DNA complementary strand. Therefore, better to design a primer with G or C nucleotide at the very end of the 3' end of the primer because it has a strong binging efficiency
Hi I have a very basic Question regarding PCR. PCR is used to amplify content of DNA. Say for ex. My sample contains 38ng/microL concentration of DNA, using PCR Am I making my solution more concentrated? (from 38 ng to 38 micro/mg?) Please clarify..
we need to amplify only one strand of the DNA,as,the sense strand contains the required fragment of DNA,then why do we need the anti-sense strand of DNA for amplification??? why do we add two primers in that case,,,,when we need to amplify only the sense DNA strand?
Sagnik Biswas Because anti sense strand is complementary to the sense strand. We actually get sense strand by amplifying an anti sense strand. This is my thinking. I maybe wrong.
Does anyone have any experience with trying to PCR with loading dye present? Or any ideas on how to get rid of it so that it doesn't inhibit the enzyme?
Basically passed all my exams from organic chem to molecular biology related subjects in uni by refreshing the contents in my notes through ur lectures. Keep it up sir! Well done!
We do watch your videos all the time and they are so much helpful for us in biotechnology sir. We do almost all the exams in the university by watching your videos. You are capable of teaching even the hardest parts, in simple ways. We like to watch videos which you yourself teach in the white board, very much. Thank you sir...bohoth bohoth shukriya!!
Thank you so much for appreciating my efforts
I wanted this a few times- thank you for breaking down the process of PCR. You don't waste time on unnecessary words or pauses like many "youtube teachers" do,
The Best and Complete PCR Explanation Video Ever...👏👏
There are 100s of Videos on PCR, But this the best...!
Thank You...!🙏🙏
Thank you so much for appreciating my efforts. Glad to hear that you're getting benefit from my lectures
i am getting hope to clear exam only because of your videos. after 8 yrs gap, i started to persue my carrier, its such a long break , ur videos given me much confidence . i hats off u. u r my role model thanks shomu sir.
Hi r u working now... I am also started studying after 8 yrs of gap hoping to get a job soon
your videos are good for brushing up concepts rather than reading all the books again when time is short. thanks.
BEST PCR LECTURE EVER🙏thanks a lot sir
You're welcome
I am very blessed found your tutorial lecture on Molecular Biology in the you tube, you gave a simple way how to understand the application and knowledge about molecular biology, you are very kind to share your knowledge to everybody in the world.....hope you get present from the God....salam from Indonesia....
thanks for perfect videos you share they're both informative and productive. It's brilliant that we have such awesome lecturers like you in RUclips who are trying their best to inform others
Thank you so much for appreciating my efforts
God bless u sir . Ur doing a grt help to thousands of people with ur beautiful videos..high quality teaching ♥️
You're welcome. Glad to hear that you're getting benefit from my lectures
IT IS SO HELPFUL!!! your explanation is so much better than my tutor's. thank you so much
Very interesting sir as you said .thank you very much for the lecture.you have provided us with very minute details which are Essential for attending the long questions ans . ❤️❤️
I didn't understand this process even though my teacher took 3 hrs to explain it.. Thanks sir for the explanation
Nice slide and explained every step in authentic way...
This is very helpful, my doctor ask us to watch your video and it best decisions ever,
Thank you so much for appreciating my efforts
Thank you so much DR RORPOPOR HERBAL on RUclips you saved my life from this deadly PCR virus, I got cured within 14daysly herpesly herpes..........
a really complete and informative video. good job and thank you :)
আপনার video গুলো অনেক সুন্দর। অনেক concept clear হয়েছে। আপনাকে অনেক ধন্যবাদ।
Thank you for appreciating my efforts
U ARE THE BEST.... HELPING ME ALOT DURING MY STUDIES. THANKS
Thank you so much DR RORPOPOR HERBAL on RUclips you saved my life from this deadly PCR virus, I got cured within 14daysly herpesly herpes..........
Thanks alot for making this subject easier may God bless you
It is so much helpful, very informative and descriptive video. Thank you sir😇
Glad to hear that you're getting benefit from my lectures
Hello sir, your videos are very helpful to all of us ( me and my batchmates )sir we all just watch your videos and we all have passed our exams with good Marks thank u soo much sir for making every topic so simple to understand. sir I am facing problem in plateau effect in PCR ,I am not able to understand it, so sir can you please help me out
Thank you. Glad you liked my lectures. Please subscribe and share
i'm starting to have hope, Thank you sir
You're welcome
Thank you sir for the good work .... I have understand the concept of PCR very easily through ur lecture, it's very helpful. . Please upload more videos related to this . Your explanation is very good ....
very helpful . The content is very detailed. Great job sir.
Glad to hear that you are getting benefit from the videos
thank you Dr Shomu
Glad to hear that you're getting benefit from my lectures
Tq so much for better understandingsir......I like ur teaching skills sir.....
Glad to hear that you're getting benefit from my lectures
it is very helpful my lecturer recommend me to watch this
Glad it helped
very very lucid explanation.
Thank you sir.
Your all videos are really helpful. Thank you so much.
Thanks...you just made everything so clear!!
hi shomu i have a question reagarding pcr reaction.. why initial pcr product produce long and varying size product in reaction, and fina reaction produced shorter and uniform size product?
Heat labile means heat sensitive. So Taq polymerase is not heat sensitive and neither heat labile!!!!
Thank you for your video.
You're welcome
Thank you very much sir...oshonkhyo dhonnobad
Thank you. Glad you liked my lectures
Got one question! when a gene is encoded on a single strand of DNA called template strand then why do we perform PCR on both strands of DNA using 2 primers?
very good technique
Very helpful thank you so much🙏🙏🙏
You're welcome
Thank you very much, very informative and helpful video
Great lecturer, thank you
Thank u very much sir. This really help me to understand more about PCR.
inspite of being such a good method of amplification why cloning is not completely replaced by pcr?
Sir I one big request.. can you make one video about enterococcus feacalis full detail
Thank you very much sir.
You're welcome
Thank you so much DR RORPOPOR HERBAL on RUclips you saved my life from this deadly PCR virus, I got cured within 14daysly herpesly herpes..........
hello tysm for this video.u r genius.u helped me in molecular biology n genetic engineering so much..can u plz provide me yr written material or books...???
Thank you very much for your video lecture, much appreciated.
Why all videos on PCR r not available at u tube???plz make them easily access for all students those who can't afford coaching...
impressive command of English
Very informative video. hoping for t same for other techniques....thanks slot it really helped me
Really very helpful sir....plse sir can u explain suicide pcr in another tutorial video.
Thank you so much sir 🥰🥰🥰🥰
You're welcome
Thankyou very much for such an informative video.. keep it up.
+Savita Ct thank you. Glad you liked my lectures
good lecture
You're welcome
A primer may bind anywhere on the DNA, obviously to ours complementary sequences but it's not always necessary that these sequences will be present at the very start. So the sequences where primer doens't bind how are those sequences amplified?
Thank you very much just a question about the oligonucléotides they are of DNA or RNA????
How sir the dna denaturate at specific site, if you provide heat it should denaturante completely?
Work well done. thanks sir
thanks a lot...very nicely explained
Sir why primase is not used ?is that bcz of small size of DNA fragment that is to be copied
Thank you so much. It was very helpful.
sir plz explain chemical. synthesis of dna & its 3 types
it was an awesome video. 😊 very helpful
excellent video
Excellent video!
What are the different enzymes used for this process
I was wondering... What would happen if the two primers were on the same strands ?? Would there be any PCR's product or nothing ?
Mike Hunt thinking same ...if we suceeded to any how artificially ....dna manuplating by pahogens could be reduced...
great job God bless you 🙌🙋
Thank you bhai 😭
You're welcome
Thank you very much! This is really helpful!
very informative
sir, i am confusing the order of the videos, recombinate DNA Technology 61 tutorials are there , but which one is 1st , 2nd..........61 videos, this is my confusing , i hope you pls clearify , thank u.
In which base pair does the primer starts binding in annealing steps? It binds to complementary sequence but in which exact bp does it bind?
It binds first at the 3' end of the primer. That's why the 5 base pairs on the 3' end of the primer are very important In order to promote a proper annealing to the DNA complementary strand. Therefore, better to design a primer with G or C nucleotide at the very end of the 3' end of the primer because it has a strong binging efficiency
Hi I have a very basic Question regarding PCR. PCR is used to amplify content of DNA. Say for ex. My sample contains 38ng/microL concentration of DNA, using PCR Am I making my solution more concentrated? (from 38 ng to 38 micro/mg?) Please clarify..
Health Support yes
hello sir, help me to clear my doubt that initially two primers are used for 1st cycle but how many primers are used for 30
cycles
very informative video
Thank you. Glad you like my lectures. Stay tuned.
Helpful..
You're welcome
Wel Done sir !
we need to amplify only one strand of the DNA,as,the sense strand contains the required fragment of DNA,then why do we need the anti-sense strand of DNA for amplification??? why do we add two primers in that case,,,,when we need to amplify only the sense DNA strand?
Sagnik Biswas Because anti sense strand is complementary to the sense strand. We actually get sense strand by amplifying an anti sense strand. This is my thinking. I maybe wrong.
You're right ☺
Why both primers ?
great video. tnx
Very nice...
Thank You!!!
You're welcome
very good.
IT IS VERY MUCH HELPFULL
Sir iss topic ki note kaise download karein
Don't have note now
very good keep it up
Does anyone have any experience with trying to PCR with loading dye present? Or any ideas on how to get rid of it so that it doesn't inhibit the enzyme?
it sounds good thanks alot
Thanks
what is means by melting temprature
thank you
I am afraid that this video is 30 min. hope i will get it
ty very much
nice
Grt👍
Thank you
what taq polymerase is important
akriti nipu coz it can resist high degree of temperature
PCR made easier thanks
nice english accent!!!
👏👏
اكو عرب اهنا 😂😂
Plz speak a little faster