Lineweaver-Burk Equation

[@kyledebruyn703]

Please show how this is done manually without excel

[@letumilechocho231]

Does this mean that the approximation method is very wrong? Because the answers for Km and V max are very different

[@dr.g5043]

Approximation is inherently flawed if your goal is to get a correct answer. However, it can be a very good guide in many instances. The dataset I show here intentionally creates a situation where the approximation is misleading because the dataset is not complete - the measured velocity does not approach Vmax. Consequently it is not possible to accurately estimate Vmax, which means the Km estimate is also flawed. Conversely, the Lineweaver-Burk approach also has some inherent issues that I don't discuss in this video which can result in incorrect Km and Vmax values.

[@hardikparmar2600]

How did you calculate Vo ?

[@dr.g5043]

These would come from individual experiments where you are monitoring the rate of the reaction. For example, if you set up an experiment that monitors the absorbance of a product and plot it vs. time, the slope of that line would be related to the rate - if this is done under controlled conditions, that would be your V0

[@tsiporacohen-taieb2195]

with using a Bio Rad plate we obtain a number that is absorbance how we can convert the Vo in concentration/time ?

[@azhietheresetangang6576]

In this video I can't really figure out the calculation of the y intercept from the graph.

[@wiwinpebriani8139]

Thankyouu,

[@degaridro]

Thank you, it helped so much now haha

[@G.p.p.G]

Perché b=0.004?