Lineweaver Burk plot data analysis

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  • Опубликовано: 16 окт 2024
  • using a Lineweaver Burk plot to analyse enzyme data
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Комментарии • 62

  • @SuperZwiebertje
    @SuperZwiebertje 12 лет назад +21

    Finally a professor that takes time in teaching you the whole thing instead of expecting that you already know 80% of it.

  • @jameshigdon100
    @jameshigdon100 8 лет назад +8

    This went from "we can find these numbers" to a solved problem.

  • @amandar3299
    @amandar3299 3 года назад +3

    I was SO confused about this - you are a life saver! :)

  • @CLEANDrumCovers
    @CLEANDrumCovers 8 лет назад +8

    Thank you, David Attenborough.

  • @makhzania
    @makhzania 12 лет назад +2

    Thank you very much for the explanation, I have found it extremely helpful in my biochemistry lab work. You explain things clearly and in simple terms which many lecturers can't see to manage. Either that or as others have noted here, they seem to assume you know most of it already!

  • @issie339
    @issie339 12 лет назад +2

    Thank you so much! I wish you were my professor! So very helpful and easy to understand. Please keep making these videos, I learn more from you, than from my lectures and books.

  • @magictrees101
    @magictrees101 2 года назад +1

    Use a computer software. Alot easier these days to get the values 😁 I love how you explained this fully! 💯

  • @Wandelaarke
    @Wandelaarke 12 лет назад +1

    Finally a professor that manages to explain this perfectly!

    • @jonahtristian5729
      @jonahtristian5729 3 года назад

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    • @owenduncan760
      @owenduncan760 3 года назад

      @Jonah Tristian instablaster =)

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      @jonahtristian5729 3 года назад

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      Looks like it's gonna take quite some time so I will get back to you later when my account password hopefully is recovered.

    • @jonahtristian5729
      @jonahtristian5729 3 года назад

      @Owen Duncan It worked and I now got access to my account again. I'm so happy!
      Thank you so much you really help me out :D

    • @owenduncan760
      @owenduncan760 3 года назад

      @Jonah Tristian happy to help =)

  • @rarasrizkialviani8379
    @rarasrizkialviani8379 4 года назад

    Hello, I'm from Indonesia, i'm biology collage student, i want to say thank you so much because this video helpfully.

  • @davidrenteria9585
    @davidrenteria9585 Год назад

    This is gold. Thank GOD 🙏
    And thank you teacher.

  • @srikantadash4603
    @srikantadash4603 3 года назад +1

    U r just a life saver for me.... Thank you ❤😘

  • @premiumedjr
    @premiumedjr 13 лет назад +1

    Very helpful tutorial! What say about Dixon's plot in determination of inhibition constants? It will be better appropriate than lineweaver-burk, once it take in to consideration just the inverse of velocity, while lineweaver utilizes both the inverse of velocity as the inverse of substrate concentration? Thank you!

  • @xanaanak55
    @xanaanak55 11 лет назад +1

    That was so unbelievably helpful. Thankyou so much. Please keep making more videos!

  • @ABDULGTV
    @ABDULGTV Год назад +1

    Thank you.

  • @Merhawit10
    @Merhawit10 10 лет назад +6

    I'm still a bit confused on how Km and Vmax were calculated?

    • @lukechess7477
      @lukechess7477 9 лет назад +14

      Reading off the Graph 1/Vmax= 0.016. This is the reciprocal, to reverse it you 1/ 1/Vmax (1/ 0.016 = 62.5) So Vmax= 62.5. You do the same for 1/ Km (1/ 0.07 = 14.3). Once you have Vmax and Km you can work out the Gradient (14.3/ 62.5 = 0.23). Again you Reverse this giving (1/ 0.23 = 4.3)

  • @Boomerangpie
    @Boomerangpie 12 лет назад +1

    THANK YOU!! this was SO helpful - a topic that has confused me for weeks :-)

  • @EdisonvsTesla
    @EdisonvsTesla 4 месяца назад

    Thanks so much, may God protect 🙏 long live

  • @chocolatepoodle6028
    @chocolatepoodle6028 6 лет назад +1

    Thank you! This really helped me in writing my biochem report. :-)

  • @robynbryant2800
    @robynbryant2800 9 лет назад +2

    Love it, thank you so much for being so helpful!!

  • @sofieStormen
    @sofieStormen 12 лет назад +1

    you saved my life :) thanks for so many details and the good explanetions :)

  • @KimHjalmarsson
    @KimHjalmarsson 11 лет назад

    This was helpful - I don't understand some of the basics still though.. Wasn't Km supposed to be half of Vmax simply? How can it be a totally different number than Vmax in the Lineweaver-Burk-plot?

  • @tsmanchester
    @tsmanchester 6 лет назад

    How did the gradient all of a sudden come up how does one work out where the gradient goes

  • @Brisbane09
    @Brisbane09 10 лет назад

    there's one thing I don't get. Our teacher told us that Km has a unit mmol/l. But the reciproke of mmol/l would be 1/mmol/l = l*mmol^-1 wouldn't it?

  • @frannnnn
    @frannnnn 12 лет назад

    GRANDE VIEJOO SOS UN GROSO ...

  • @SUNNI3DAY
    @SUNNI3DAY 12 лет назад

    Very helpful! Saved my lab write up.

  • @GHOST3940136
    @GHOST3940136 6 лет назад

    nice videos Peter, thx, that cleared some things up.

  • @1705ika
    @1705ika 11 лет назад +1

    thaaankk youuu so much Peter Kappa, you really help me, you safe my life :')

  • @kathxlin
    @kathxlin 10 лет назад

    Thanks so much!!! This really helps heaps, Dr Klappa. Cheers! =)

  • @apvert
    @apvert 12 лет назад +1

    Extremely helpful, thanks!

  • @dunyalaftamohammedkufa6908
    @dunyalaftamohammedkufa6908 2 года назад

    What about ki of non competitive inhibitors

  • @sweetestseed
    @sweetestseed 11 лет назад

    Thank youuuuuu you're a life saver! God bless you

  • @Nadasistschoen
    @Nadasistschoen 11 лет назад

    Thank you very much, this was incredibly useful!!!!

  • @asumangulsumkaya2887
    @asumangulsumkaya2887 6 лет назад

    still I couldn't understand where Vmax come from? He said that it is 0,016 from graph but later he says it is 62,5??? Btw thank you for this enlightening video

  • @alvaro811994
    @alvaro811994 13 лет назад

    thanks a lot...it really helped me..keep it up!!!

  • @ssoblasted
    @ssoblasted 12 лет назад

    thank you, this really helped my lap report.

  • @maevecahalan1938
    @maevecahalan1938 12 лет назад

    Excellent!!!!!!!

  • @jmse7en77
    @jmse7en77 10 лет назад

    Thank you, it was very helpful!

  • @getfiasco
    @getfiasco 12 лет назад

    i get how you work vmax but how do you work out km :s

  • @dbwun
    @dbwun 11 лет назад

    Great video, thanks!

  • @حوراءحيدر-م2ذ
    @حوراءحيدر-م2ذ 4 года назад

    Thank you so much

  • @MrFilu13
    @MrFilu13 12 лет назад

    thank u , it is very helpful

  • @88abuk
    @88abuk 12 лет назад

    thnx very much
    really helpfull

  • @tajikamman
    @tajikamman 11 лет назад

    very helpful thanks so much! :-)

  • @039stephen
    @039stephen 5 лет назад

    40:41 jack, its called sleet

  • @BioScienceClass
    @BioScienceClass 4 года назад

    For more videos on Enzyme Kinetics:ruclips.net/video/wAWYIGTRXQE/видео.html

  • @gamzeyazgeldi6925
    @gamzeyazgeldi6925 6 лет назад

    Thanks!!

  • @getfiasco
    @getfiasco 12 лет назад

    oh i get it now. very helpful :)

  • @andienlovehanif
    @andienlovehanif 12 лет назад

    LIKE THIS BANGETLAH CC: NIDA MAHDA ANIDA AHLI PERHITUNGAN DI SETIAP LAPORAN

  • @misschocolate383
    @misschocolate383 8 лет назад

    thank you sir

  • @rajkishore5334
    @rajkishore5334 10 лет назад

    nice talk..

  • @EddyKackboon
    @EddyKackboon 7 лет назад

    very cool =)

  • @adrianculasing1279
    @adrianculasing1279 6 лет назад

    Bruh thank you.

  • @poppyflower333
    @poppyflower333 11 лет назад

    Thanks,

  • @ryanj1304
    @ryanj1304 12 лет назад

    @9Dan22
    62= vmax
    14= km
    calculated from 1/0.016 and 1/0.07 :)