How to calculate Km and Vmax values - Lineweaver Burk plot in Excel

This video explains about How to calculate Km and Vmax values - Lineweaver Burk plot in Excel.
Km and Vmax value calculation in excel from the enzyme kinetic data. Michealis menton constant (Km) can be accurately calculated from the lineweaver burk plot in excel.
Enzyme Kinetics: Lineweaver Burk Plot Explanation:
• Enzyme Kinetics : Line...
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[@mr.legend368]

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[@alphazenturbo]

Very straight forward. I needed to a L-B for Pchem and this was extremely helpful for making the graph in Excel.

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[@beehyve]

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THank you for making this video! IT was so helpful!!

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[@blabiblabiblabi]

Thank you so much. I did an experiment to determine Km. Now i know how do calculate it

[@aishwaryaramkumar1221]

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what a life saver, explained it very well

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The explanation is really very helpful

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[@rakshamukhia7970]

Sir, I do not understand but Iam gettingthe equation plotted in the graph with a ngative sign. where iam I flawed? y=10.192x-0.3494

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[@rspeks9461]

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[@rspeks9461]

Got me through my chemistry assignment!

[@zaysiz]

Sometimes, I get Lineweaver-Burk equation as y=ax-b. While 1/Km is possitive, 1/Vmax is negative. Is it problem? Why is it negative?

[@mariebrassart9868]

Very clear and helpful, thank you ! 😊😊😊

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[@aelafalzabede6064]

4.3002 how i can result this?? i dont know 😢

[@valenciajoanna3965]

Same question

[@justflow.77]

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[@songulates7833]

Thank you so much but i didn't understand , why is backward=0.5 periods for forecast?

[@Not-Nur]

perfect video, thank you

[@DeStIiNix99]

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[@berkayadem7197]

thank you very much

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[@zaidmaniar2899]

how did you get the initial velocities/product formed ?

[@brianbridges8124]

hello, why do you bring the line to - 0.5, im wondering how u know what vmax is?

[@nikitarashinkar1897]

Thank you 😊

[@samanthac323]

This was so helpful. Thank you!

[@BioResource]

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[@misbahhusolli]

I found difficult when use this plot for bacteria fermentation kinetic, where the curve of 1/s and 1/u are not the same as the literature. the line of the curve is downward, Y = -ax + b, but the R square = 0,9 kind of. what if the result of this plot is downward curve, is it still can be use as a data to calculate Km?

[@ximena5063]

No

[@malleshgampa1956]

Substrate concentration we have to take in mM?

[@maxw5900]

Thank you, Good Sir!

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Very nice!

[@michellemandelstam8633]

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[@zzziii-qr5io]

Why choose backward 0.5?????, can someone explain

[@danielhuelgas5355]

Thanks a lot!

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[@khadijahahmed1970]

Thanks mate

[@deepaksshanawad488]

How to calculate product formed values?

[@foreverninteen8580]

What is the unit of substrate concentration [S] here?

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[@solomonabioye1516]

Thanks for the video but I still need help pls

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Nice

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Ak numerical solve krvna ta

[@handlelector]

2:15

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