Hello! Just wanted to leave a quick comment thanking you for your excellent video. It is a godsend! I couldn't wrap my head around the topic until you explained it in such a simple and concise manner. Kudos to you!
when S is very low, V = 0 right? Or V = (Vmax/Km) x S, but S is very small so V must be very small as well, where the graph is almost linear when S is small, but gradually curved into plateau when S became big enough.
This is the greatest explanation on Michaelis-Menten Equations ever! Thanks and be blessed for this simple plausible explanation it's just straight forward if you know what you're doing🎉❤
🇺🇸 As an electronic technician this looks very similar to an electron bias on a semiconductor silicon or a geranium substrate junction I pause to see if there's any similarities between conduction by electron transport Although I do realize it's a molecular system
Great video... not many other youtube channels for biochem out there, so keep it up! ***Heads up for American viewers: when "into" is said in the video I believe it means "multiply". In the U.S. we say the opposite, "into" is for division, so that might be confusing for some people
5:53 What is the constant Km referencing as a mathmatical construct? A process from reaction to and from equilibrium states ? Denoted as a Constant called Km? Is this Km constant the reaction times (lay person terms to understand the process) mathmatically created to allow a formula to prove itself mathematically called the mechaelis menden equation? Ty
Thank you for your question. Km is the ratio of the rate constant related to dissociation of ES complex (KR and Kcat) to the rate constant that forms the ES complex (KF). Practically it is calculated as the substrate concentration at which velocity becomes half of Vmax.
OK that's all fine but what do you use it for practically? What can you use it for? It's just math for the purpose of math. I imagine Km is interesting because it represents the concentration at which 1. order kinetics is relevant, and thus a linear regression is achieved.. Which I guess can be used to calculate backwards if you're trying to figure out the concentration of a an unknown, ie. a blood sample or other.
Thinking a bit longer what is the propagation? @ the electron proton particle level transfer? Perhaps I'm looking at it all wrong at the enzyme level? If someone could explain or point me in the direction where I might be able to fill in that Gap.... I would appreciate it Thank you, a fascinating subject
I think at 2:45 it is important to understand the Kf[E][S]=Kr[ES] is referring to the assumption at equilibrium the RATE of formation of ES and dissociation of ES is equal. Kf[E][S] is the association constant (Kf) * Enzyme concentration at equilibrium [E]eq * Substrate concentration at equilibrium [S]eq. Same logic for Kr[ES]eq. That is how that equation came up.
The break down of Kcat[ES] is due to the fact that the enzyme substrate complex will break down and form just enzyme and just product. so you wouldn't have EP because there is no enzyme product complex. the only thing that is experiencing "Breakdown" is the enzyme substrate complex, so [ES] will be the only part of that equation.
Thank you for the channel. Can you please make a video about single displacement, double displacement and ping pong enzymatic reactions. If not, can you please direct me to a good source. Thank you in advance
the rate constant for forward and reverse reaction of enzyme and substrate. It's more of a metaphorical value in this particular video, but it's used to measure rate constants throughout interaction and all go into calculating Km
Hello!
Just wanted to leave a quick comment thanking you for your excellent video. It is a godsend! I couldn't wrap my head around the topic until you explained it in such a simple and concise manner. Kudos to you!
On the other hand, if the concentration of substrate is very low ([S]
when S is very low, V = 0 right? Or V = (Vmax/Km) x S, but S is very small so V must be very small as well, where the graph is almost linear when S is small, but gradually curved into plateau when S became big enough.
Really really easy explanation.. I was so lost before hearing this. Now I can understand very well!!
Hi
Maybe i'm just tired, but this explanation established no clarity for me. Were you just deriving various equations from the M.M. Equation??
Replay the video , it will give clarity . And think about the stuffs more and more , like how things are going on inside.
Yes you are right
And my 1 like made it 100
You were just tired. Cause I'm also very tired right now, and it does seem like he's just deriving equations.😂
@@lekanganambela5183 😂
This is the greatest explanation on Michaelis-Menten Equations ever! Thanks and be blessed for this simple plausible explanation it's just straight forward if you know what you're doing🎉❤
Thank you so much, I never understand this after taking biochem for a semester
🇺🇸
As an electronic technician this looks very similar to an electron bias on a semiconductor silicon or a geranium substrate junction
I pause to see if there's any similarities between conduction by electron transport
Although I do realize it's a molecular system
a lot of molecular reactions occur through electron exchange so i’d imagine the equations reflect the similarities
Crystal clear explanation but you need a calm mind to get it at one go 🔥
hats off you sir making such a conceptual , easy video for us
Great video... not many other youtube channels for biochem out there, so keep it up!
***Heads up for American viewers: when "into" is said in the video I believe it means "multiply". In the U.S. we say the opposite, "into" is for division, so that might be confusing for some people
Nearly all the English-speaking world would use 'into' to mean divide
@@eileenhenryselby-smith9762😮 into ka mtlb tu multiply hota h xd
@@eileenhenryselby-smith9762 really ? From childhood we use into as multiplication
The best explanation for this equation
i AGREE
That is a very nice description, thank you so much!
Thank you so much for teaching it in a really easy way. May God bless you! ...
This video is extremely helpful & so well explained ....😊Thank you sir
Thank you so much sir. It all makes sense to me now
Wow..this is super helpful and you explained very well..❤
Thanks so much for your contribution to my progress 🙏🙏🙏
Thank you so much I wish you could be my professor one day ohhhhhhh I really appreciate this work. God bless you 🙏🙏🙏🙏
you destroyed it!
thanks a lot my guru
Very nice, everything about these are in the textbook but sometimes we need someone to read it for us in order to understand.
5:53
What is the constant Km referencing as a mathmatical construct? A process from reaction to and from equilibrium states ? Denoted as a Constant called Km?
Is this Km constant the reaction times (lay person terms to understand the process) mathmatically created to allow a formula to prove itself mathematically called the mechaelis menden equation?
Ty
Thank you for your question.
Km is the ratio of the rate constant related to dissociation of ES complex (KR and Kcat) to the rate constant that forms the ES complex (KF).
Practically it is calculated as the substrate concentration at which velocity becomes half of Vmax.
thank you so much, for such a clear cut explanation
why the M-M equation in the enzymatic reaction focuses on V0 (initial velocity)?
Thnku sir it's so useful and helpful video I ever seen about this topic
amazing, thank you! God bless you sir.
Too good explained ..Thankyou sir!
Hi
Bagus penjelasannya. Terima kasih kawan
Great video, thank alot. Please what does Kcat stand for?
Nice explanation
Thank you for explaining so easily
Excellent. Thanks Sir.
Thank you so much,your video was extremely helpful bro.
great video, thank you so much
Great explanation sir..tq
will a large or a small value of Vo have a greater impact on the rate of the reaction?
I have a question?
How you can relate reaction rate with the substrate
concentration from michaelis-menten equation?
Please respond 🥺 urgent
im so failing this semester. How do I create a MM curve from absorption that I found using a spectrophotometer
Using Excel solver and model fitting.
Could answer that, but it is too late now I guess
@@nwoadrenochromdealer6667 only 2 years🤣
this man is a hero
OK that's all fine but what do you use it for practically? What can you use it for? It's just math for the purpose of math. I imagine Km is interesting because it represents the concentration at which 1. order kinetics is relevant, and thus a linear regression is achieved.. Which I guess can be used to calculate backwards if you're trying to figure out the concentration of a an unknown, ie. a blood sample or other.
For the velocity what is “d”?
Thanks for ur teaching skill....❤️❤️
Made it so easy to understand 👍
Really great buddy ....
Pseudo steady state means??
Excellent 👌🏼
thanks so much for the video
really helped me a lot because now it makes alot of sense
Thanks, this was really helpful
Your voice sounds exactly like my dad’s lol. Not sure if that’s a good thing or not
He is your dad
@@ribble6381 yes once you should check
Call him DADDY
@@Dr_108 fumny
Why minus sign is kept in this equation ?? -du/dt ?? Why minus sign ??
because we spend the reactants thats why we use -
. we produce the products thats why we use +
because the substrate is decreasing and the product is increasing.
Thinking a bit longer what is the propagation? @ the electron proton particle level transfer?
Perhaps I'm looking at it all wrong at the enzyme level?
If someone could explain or point me in the direction where I might be able to fill in that Gap....
I would appreciate it
Thank you, a fascinating subject
what about second order and third order kinetics?
I think at 2:45 it is important to understand the Kf[E][S]=Kr[ES] is referring to the assumption at equilibrium the RATE of formation of ES and dissociation of ES is equal. Kf[E][S] is the association constant (Kf) * Enzyme concentration at equilibrium [E]eq * Substrate concentration at equilibrium [S]eq. Same logic for Kr[ES]eq. That is how that equation came up.
i love learning things from kermit the frog (jk this was very helpful thank you)
Very good explanation
This video can increase your confusion of this topic
what is Kf Kr and Kcat?
I don't get it no matter how hard I try
Best way to explain
Amazing explanation, thanks.
Thank you sir it's very useful to me
The ratio of Kr/Kf should be [ES]/[E] [S] and not [E] [S]/[ES].
Amazing video
Excellent 👍👍
Explained wonderfully 👍
Can you please tell me why ES breakdown =Kr(ES) +Kcat(ES)????? Instead of Kcat(EP)??
because it is breaking down by forming products and reforming the reactants
The break down of Kcat[ES] is due to the fact that the enzyme substrate complex will break down and form just enzyme and just product. so you wouldn't have EP because there is no enzyme product complex. the only thing that is experiencing "Breakdown" is the enzyme substrate complex, so [ES] will be the only part of that equation.
What is Vo ?
Brilliant..🤩
You are my champion. My love even.
do medical students study this, its not in A level biology?
Thank you!
Thankyou for this... 👍
crazy, how they know that before 100 years ago
Thanks very much for this detailed explanation 😊
allah razı olsun abicim
EEEEEEEE ehhs EEEEE ehhhhs, love the video it helped so much but that muddled my brain so much hearing that repeatedly 🤣
Thanks❤️❤️
thank you so much
I understand nothing.
This look exactly like Langmuir isotherm
Thanks very much
good bro.... i mean very good thanks
Thank you for the channel. Can you please make a video about single displacement, double displacement and ping pong enzymatic reactions. If not, can you please direct me to a good source. Thank you in advance
Thank you
Guys what is the kf and kr there
the rate constant for forward and reverse reaction of enzyme and substrate. It's more of a metaphorical value in this particular video, but it's used to measure rate constants throughout interaction and all go into calculating Km
Maybe am tired too maybe it's the laziness in his voice
did you know that 9 grade are learning it
I got more confused after watching this
わかりやすいけどむずいよおおおおお
Yeeeasssss....
Iam confoced
i only hear re zero instead of v0
Make your video .
Why do you want to demoralize people .
Can you make your tutorial please
i still don't understand, fuck sake :(
This has no buisnes bejnb this complicsed. Exam in 6 days ready to off myself
Thanks... For nothing
Haa
I am enzymologii and chemist together. Terrible.
Wtf
You can not Say as you have dane. Please learn a bit of chemistry . Terrible to hear being chemist.
Good. But, your accent is quite annoying.
Very nice explanation