Lineweaver Burk plot

A typical curve of enzyme kinetics is a plot of a plot of velocity of reaction vs substrate concentration. As the substrate concentration increases the velocity of the reaction increases until it reaches a plateau, which is known as maximum reaction velocity or Vmax. The drawback of this graph is that the plateau region is not perfectly horizontal. Beacuse of this there is a error in the estimation of Vmax and Km. Hence the researchers started modifying the graphs to get better estimate of Vmax and Km. One of such graph is lineweaver burk plot.

[@rachael4775]

I remember watching this in university and I never forgot how helpful it was. Now 4 years later, I'm coming back to it in medical school

[@niatai9651]

Same!. Except for Pharmacy school

[@Lulu-uw1mj]

the video was posted 2 years ago...

[@rakshitasingh6574]

@@Lulu-uw1mj 😅

[@olaoluwaoyeniyi9948]

​@@Lulu-uw1mjsarcasm bro

[@blueflash3641]

hey what was your bsc in ?

[@theinkedaaila]

This is perfect to understand whatever the hell is this lineweaver burk plot...... just wow

[@speci4list326]

I was procrastinating because this seemed really hard to understand. Thank you!

[@Woogie9593]

*watching video after getting all lineweaver burke questions wrong*
"hence, lineweaver burke plot is one of the easiest graphs to understand enzyme inhibition"

[@alonzojonathan2553]

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I was stupid lost my account password. I would appreciate any assistance you can offer me!

[@dustinameer4343]

@Alonzo Jonathan instablaster =)

[@alonzojonathan2553]

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[@alonzojonathan2553]

@Dustin Ameer it did the trick and I finally got access to my account again. I'm so happy!
Thanks so much, you really help me out!

[@dustinameer4343]

@Alonzo Jonathan you are welcome xD

[@bobmogoboya1477]

Yoh!! im writting tomorrow and i thought i was gonna fail this burk plot and now i get it. Thank you!!!!

[@BioScienceClass]

More Videos on Lineweaver Burk plot: ruclips.net/video/wAWYIGTRXQE/видео.html

[@surbhisharma704]

Thank you so much for making it that simple for understanding in a better way

[@jyotsnaraut77]

I thought it's very hard
But seeing this video clear all the doubts thank you ❤️

[@gayatri5996]

Sometimes the easiest math seem difficult @ 2 o clock at night. Thanks for making it clear!

[@Drift1]

Thank you! the mathematics explanation helped explained how Vmax and Km value changes can be infered from the Line - weever. I guess thats the point of it, make it simple to see changes.

[@stellawanjiku4362]

Thanks, this made it so much easier to understand

[@nicolevillalon9737]

Quick and excellent explanation. Thank you!

[@katejbradshaw]

THIS video makes it all clear! THANK YOU!

[@MzPeregrine]

You are probably going save my exams today

[@Crystalrosety]

I thought this was hard u made it so easy thank u

[@AA-kr1ci]

Explained to perfection !

[@nyikomhlarhimusic]

Awesome explanation. Thank you veeeery much 🙏🏿

[@peerzadaabrar1800]

Very very Thankful to you. Jazakallahu Khair🤲.

[@heybriaannaa]

great video! thanks for sharing!

[@itzelhidalgo874]

Fantastic video!!
Thank you so much 😉

[@elijahperry3454]

Lifesaver...thanks a bunch!

[@mayryelll3822]

the only video that i understood thanks alot 😭🙏

[@malithaniman1730]

Helped a lot thanks

[@sanskritimishra7299]

This would help in my presentation ! ✌️

[@jokebotter-vanelburg8590]

where does -1/Km come from?

[@nancybarajas4249]

At the x-intercept, the y-intercept is equal to zero. Using the lineweaver burk equation, you set 1/Vo equal to zero and solve for 1/[S] (x-int). This will give you -1/Km

[@JANVI_743]

@@nancybarajas4249 thank you, it was useful

[@SilvanaLeonMeza]

so helpful! I’m an italian med student

[@kamariweaver1536]

What values on the plot are Vmax and what value is Km?

[@TempleofYHWH]

fr 😭

[@easyochem]

This is a certified hood classic

[@logitfau252]

thank u alot :) really helped me for my assignment :)

[@harshithjagadeeswaran527]

Was really helpful.thanks 😊

[@محمدرحيم-ف5ز]

Plz
-1/km
From where coming???

[@abhishekrana5933]

Thank you from 🇮🇳

[@siddheshpathak9181]

But on X axis why do we take - 1/Km ?

[@krutarthshukla3067]

keeps bugging the hell out of me

[@paritiyasingh5342]

Since y=0, MX=-c. Then x= -c/m. Substitute values to get. X=-1/Km. Because Vmax get cancelled

[@Obamnaz]

@@paritiyasingh5342 Tanksss

[@abhi9973]

@@paritiyasingh5342 nice.thx

[@prismaticseal2553]

I understand the how, not the why or what are any values

[@alyssatipler6980]

I dont understand how you get the -1/km as the x intercept

[@gfkenya9926]

see
below from her post. Best to you.
"Nancy Barajas
1 month ago (edited)
At the x-intercept, the y-intercept is equal to zero. Using the lineweaver burk equation, you set 1/Vo equal to zero and solve for 1/[S] (x-int). This will give you -1/Km"

[@mckenseymiddleton9166]

that helped me a lot, thank you!

[@Moye_moye03]

Thanks🙏

[@AmberU]

Just maybe I’ll make it through biochemistry

[@rosekendi8590]

Thankyou easy to understand

[@jen-hungfang5437]

awesome channel!

[@tarunkumarpalli7580]

How -1/km is come on negative side of x axis

[@gatwiringera7567]

thank you very much. Understood!!!!!

[@mayryelll3822]

god this was soo easy thank you sm

[@ayaahmedmahersayedabdelaal242]

This is so good. thank you!

[@preethimurugesh6722]

Simply superb

[@oumaimaboughazala5706]

Thank you so much ,I understand it

[@linam4848]

Great stuff

[@tylerdurden7903]

Great video

[@tukurummulkhairi9794]

Thanks a million times

[@richardshane456]

Reverse hardware engineering use similar mythology to reverse engineer to find the answer incrementally by best guest trial and error feedback logic
a natural humanistic approach to solving a problem with the Mathematical mythology of trial and substitute to understand the trajectory spin of any reaction mathematically incrementally with finer granularity of the understanding of any process...
A common mistake with observation by maintaining a relative frame of data points that in reality are trajectory spin...
Although the graph inferring to the deduc enzyme reaction metric, belaboring the point of graphical representation for a finite metric that does not disclose the granular processes of the line depicted as the rate of change of velocity.
It would be interesting to observe the elementary particle processes of a enzymatic reaction...
Ty...very interesting
Good day

[@prakritipurushottam8427]

You got 1 subscriber today

[@ahmedshah6277]

You are not telling about what is meant by these values and how it changes with substrate concentration so its very hard for me to understsnd

[@asiaaaa]

awesome, thank you!!

[@munivet6685]

Excellent

[@abdullahshah7644]

outstanding

[@confucheese]

Weemax

[@myra9250]

ThankYou.

[@lovekenmaforlife3406]

Thank youuuuuu

[@emilyi771]

Ty

[@vaishuu04]

Thank you!! It really helped!!!

[@MuhammadAli-fj8qh]

1:51 I hate y=mx+c

[@sebastianlerette9450]

🙏

[@alhaeri1]

Legend

[@arnepetric7333]

sef razjasnu

[@anchalyadav3061]

Thanku

[@justicedauda6117]

❤😊😂

[@Blaskhartor]

Are you a man or a woman??

[@skellingtonmeteoryballoon]

IF we invert the equation.
Can vmax and km should be inverted to plot in the graph. Graph calculator is more than a convenience 🧮