Gary, I’m pretty sure you are the only dude on RUclips doing this kind of in depth content. Believe me, I’ve searched. We appreciate it. I am wanting to understand the process of breeding more and there just isnt a lot of content out there.
Great video Gary! This makes me wish someone would make an iso printer that could print single spores perfectly spaced out on agar plates. Would love to see a video about strengthening genetics and principles of inheritance.
I watched your video where you divide a spore solution multiple times until you get a low enough spore concentration to easily isolate single spores. I thought a bit and you might be able to do the same thing with sticky tape. Make a spore print on the sticky side of sticky tape, then line up a bunch of sticky tapes in a row and touch the spore print side to a fresh piece of tape sticky side to sticky side and pull it apart which probably removes half of the spores then grab a fresh piece of sticky tape and touch the last piece of tape together with the new piece. Eventually you get down to single spores. Not sure if a single spore on a piece of sticky tape is useful but was an interesting thought exercise.
Such highly valued info that very few people ever show or even know how. You are a master Gary and your expertise and knowledge really is endless. Thank you for taking the time to teach others.
@FreshfromtheFarmFungi I'm working on the organizational part myself. Do you happen to know is there is such a thing as a screw on lid for petri dishes? I hate having to tape everything up haha.
After isolating a haploid, and growing it out on agar, and combining it with another haploid, will the entire monokaryon become a dikaryon? Or only where they fuse together? Can you make a transfer from the farthest side and it still be monokariotic or does information travel through and know it has mated?
What is the advantage of isolating single spores over allowing spores to randomly mate on a media plate or other nutritious matter? Is there a way to determine which spores will have better genetics?
the advantage is that you can get more variance - with random selection the stronger genetic expression will prevail and you end up with similar genetics (not necessarily a bad thing)
Not really, I am making a better process and filming currently- it has been a couple years in the works I don’t enjoy tedious microscopy but I do enjoy making videos 🙏🏻🍄❤️
First time i hear about this is from you. Imagining a cross between cordycep militaris and lions mane that grow very well in wood and eat pests. Also it would look like a cordycep stalk with lions mane cap or reverse :)) This have crazy potential imo.
Gary, I’m pretty sure you are the only dude on RUclips doing this kind of in depth content. Believe me, I’ve searched. We appreciate it. I am wanting to understand the process of breeding more and there just isnt a lot of content out there.
thanks for watching and following along! I am building up to a new method of breeding can’t wait to share! 🙏🏻🍄❤️
@@FreshfromtheFarmFungi great videos!!!!
Is the new method out yet?
This is the one I've been waiting for! Cx
It's because Gary is playing with fire, isn't it? Lol
Them is some skills, my hands just aren't steady enough.
Now I gotta figure how to justify buying a microscope to my wife 🤣 she'll understand 🫡
Shit man if it's business purchase she doesn't really deserve a say 😂😂
thanks for sharing Gary!
Thank you for another great informative video Gary.
Gary is finally showing his gangster stripes. Digging the metamorphosis, also I knew he wasn’t just some gourmet cultivator.
This is why you're becoming my favorite to learn from
thanks for the kind words! MUSHLOVE
Super cool technique!
Thank you so much for showing how to narrow a Pipette.
I didn't know that this was possible till now.
learned it in 6th grade chemistry class I
am thankful to have had such a trusting instructor looking back on it! 🙏🏻🍄❤️
Great video Gary! This makes me wish someone would make an iso printer that could print single spores perfectly spaced out on agar plates. Would love to see a video about strengthening genetics and principles of inheritance.
hah glad someone figured it out 😎 this is my exact project! Kind of… stay tuned once I get the funds I will work on this 👍
I watched your video where you divide a spore solution multiple times until you get a low enough spore concentration to easily isolate single spores. I thought a bit and you might be able to do the same thing with sticky tape. Make a spore print on the sticky side of sticky tape, then line up a bunch of sticky tapes in a row and touch the spore print side to a fresh piece of tape sticky side to sticky side and pull it apart which probably removes half of the spores then grab a fresh piece of sticky tape and touch the last piece of tape together with the new piece. Eventually you get down to single spores. Not sure if a single spore on a piece of sticky tape is useful but was an interesting thought exercise.
Such highly valued info that very few people ever show or even know how. You are a master Gary and your expertise and knowledge really is endless. Thank you for taking the time to teach others.
Hey Gary, what's a good reference for identification based on size and other spore attributes?
Bro before the video even started I saw the spore print in the petri dish and I woke up my daughter saying "that's effing genius" lol.
thanks! I have a large library and making them in dishes also helps organize 🙏🏻🍄❤️
@FreshfromtheFarmFungi I'm working on the organizational part myself. Do you happen to know is there is such a thing as a screw on lid for petri dishes? I hate having to tape everything up haha.
Great video😁
After isolating a haploid, and growing it out on agar, and combining it with another haploid, will the entire monokaryon become a dikaryon? Or only where they fuse together? Can you make a transfer from the farthest side and it still be monokariotic or does information travel through and know it has mated?
Awesome video
Omg when you think Einstein figured it all out , somebody comes and says hold my beer
What is the advantage of isolating single spores over allowing spores to randomly mate on a media plate or other nutritious matter? Is there a way to determine which spores will have better genetics?
the advantage is that you can get more variance - with random selection the stronger genetic expression will prevail and you end up with similar genetics (not necessarily a bad thing)
MAP gas is sold in yellow, propane tanks are blue
Awsome
Can this be achieved with a hypodermic needle?
yes I think with a steady hand
I was wondering if anybody went through this kind of trouble to get haploid spores. Do you make a practice of doing this?
Not really, I am making a better process and filming currently- it has been a couple years in the works I don’t enjoy tedious microscopy but I do enjoy making videos 🙏🏻🍄❤️
Is an isolated spore syringe liquid culture?
no liquid culture is different- watch this video it may help ruclips.net/video/x1b1hEX8ABM/видео.htmlsi=5iM4QRnXMocRt_GM
Bro!!!
It was valentines day...did you let it meet is honey? Or is it still lonely?
Bro, it's a MICROscope, you only need one drop. Not 5.
First time i hear about this is from you.
Imagining a cross between cordycep militaris and lions mane that grow very well in wood and eat pests.
Also it would look like a cordycep stalk with lions mane cap or reverse :))
This have crazy potential imo.
hah yes but would be very difficult to cross ascospore with a basidiospore - genetic modification required 🧏♂️
was hoping for mor than 100x
that would require an incredibly steady hand haha but challenge accepted!
true lol@@FreshfromtheFarmFungi