Measuring Mushroom Spores with a Microscope
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- Опубликовано: 15 окт 2024
- This video runs through the procedure for measuring a spore using an Amscope compound microscope with a digital camera. Enjoy and MUSHLOVE!
Amscope link
AmScope - 40X-2500X LED Digital Binocular Compound Microscope
amzn.to/3j93sLw
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I got a shipment of those wine caps you made. Got 300ml of amazing looking LC from it and 2 amazing looking 600ml jars of popcorn that I'm gonna spread out in a month or two. That's only half the syringe!! Thanks Gary!!! Mush Love
Gary is one of my favorite mycologists. Gary and 90sm are great people and the other people out there should take notes
“we need to follow the science” In “Fraudci’s” voice.. lol Thanks for following along! MUSHLOVE
Checkmate people who said we will never use geometry again, after school, in our daily lives 😜
I think the Pythagorean theorem and the percentage formula are probably the two I've used the most throughout my life since leaving school. They're so incredibly useful. Great for determining the height of a tree too.
this is a great use for my latest OpenFlexure microscope!
Alan Rockefeller has a video on measuring spores & it had a very simple measuring tool in it like a ruler.
his video may be on the "crime pays but botany doesn't" YT page
that seems like a lot of work you have to do to get a simple measurement.
that ruler would need to be calibrated before hand, so yes it is easier but this microscope did not come with a micrometer in the objective which is why I showed this method. There are three different ways to measure spore for the most part. Wet prep (this way) dry slide which is probably what he did and with an EMS
Hi. Great video. I'm a bit lost on the maths though. Could you explain where the 27.5 came from? Thanks
Really cool stuff gonna try that thanks!
Although it's been many years since I studied algebra, it came back very quickly.
I have to ask Gary, do spore sizes alter that much between strains!!!
yes they can - spore sizes, shapes, cell wall thickness etc.
Very interesting, thanks for the great work and sharing it !
great work.
Brilliant
Hey Gary! What software are you using for live viewing?
Ever thought about the concave drop, thanks
Can the camera be used with the 25x oculars that the scope comes with for greater mag? Or is that only possible for direct viewing
@@BigfootPrinting It doesn’t fit mine but maybe there is an adapter Im not using that came with it
Can you test your spore dilutes under the microscope to experimentally test the spore concentrations?? It would make a great video
yes! I will do this!
Hey there. I never really make comments but I have a very important observation.
I ended up with an okay microscope with one of those usb eye piece cameras. I think the camera is similar to the one you have, but not identical. I have wondered how people took measurements and have heard some ways to guess, but this video was the first time I heard of a method like yours.
My eyepiece field of view is much larger than it is with the camera. My camera does not have the exact same field of view as my eye pieces.
I think its inaccurate to attribute the numbers on the objectives and eyepieces to the field of view that is present in the camera. I think your method holds up as a decent estimate, but like you said its "pretty subjective at that point". I like this method of guessing, but I do not think it is "much more accurate" than other methods. I think maybe some improvements could be made if we had more information about the camera, or maybe just the difference in FOV between eyepiece and camera.
Maybe I'm wrong or have the wrong camera, either way, lets discuss this. Thank you for your work with mycology and microscopy, I enjoy your videos.
I will test this
How do you transfer a spore print to a dish? The only option i can find is spore print to foil. As always thank you
I make them in used or broken dishes and store them in stacks 👍 you can do the same procedure off of foil though it’s all relatively the same 🍄❤️
Gary what is your background? Do you have an education in mycology? Or was it just a hobby that took off?
I am formally a certified medical lab tech but started as a hobby about 7 years ago
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I'd really look elsewhere for information on microscopic measurements! As a biologist, I find his explanation really simplistic and not necessarily realistic. There are a lot of other variables with computer screens and camera hookups that are not integrated. Pythagorean theorem for a rectangle? Some folks should be reading rather then writing!
why measure a spore.. when you never ever will find out how intelligence behind does the magic.. by the way you never grown a shroom.. but the ego is telling you that
to identify an unknown mushroom or compare species- I am an observer 🙏🏻🍄❤️
@@FreshfromtheFarmFungido you really believe you grown a single mushroom ?